Q9FB27 (Q9FB27_9ACTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Peptide synthetase NRPS9-8 EMBL AAG02355.1

Gene names

Name:

blmX EMBL AAG02355.1

Organism

Streptomyces verticillus EMBL AAG02355.1

Taxonomic identifier

29309 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	2162 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Gene Ontology (GO)
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9FB27 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D9F0CAB3C79A6D96	FASTA	2,162	232,404
	10 20 30 40 50 60 MPRCARGALS TAVHTTRQGS WNVMETANSG YRVSPQQRHL WAMLTRGRDG GRRAFTQSAV 70 80 90 100 110 120 VVDRSLDAAR LRAALASVVA AHEPLRTTFT GLAGRTAPVQ VVHDPDEQPL SVVDLPPSCA 130 140 150 160 170 180 DGSGPELDEL RLRERAALDP RGGPVFRAAL ARAGEDRAVL VLTAHALVAD RLSLRLLAGQ 190 200 210 220 230 240 ILAAYSGETV SPDGPPPLQY ADFAAWHHDL LTAEDAAPDR AHWAAHTATA GTGPLPGVVR 250 260 270 280 290 300 PGAAPGPWRA REWELPAELV AGIDGVAGKL STDPATVLHA AFRIAVWRLA GERNLPVALT 310 320 330 340 350 360 RDGRSHPELR TAIGAFEREL PLVHEIRHET AFAEYARALD ALVAEGEELL DHCDPELLGS 370 380 390 400 410 420 LDGTAEGPCF TFTHHQAETP VRRAGITFTT VHQDSGTPIP VRLTARRDGA RLRMELGYDE 430 440 450 460 470 480 GRIDETFPEN AAACLTRILE GVVSAPEGPV GDIRMLSDET ARLLREAGLG PRVELPGKAV 490 500 510 520 530 540 HELFAEQAAR TPGAVAVSAG EDALTYAELD ERSNRLAHHL TGLGVTPGRH VVVSVGRSAE 550 560 570 580 590 600 LLVGLLGVLK AGGAFVPVDV GFPRKRLEFV LRETAAPVLL CTADVRDRIG TRTLDDAGVT 610 620 630 640 650 660 PVALDADRRR IAAHPAGPTG IATTPDAPAY VVYTSGTTGK PNGVRVPHRG LTNYLTWCTG 670 680 690 700 710 720 AYGLDGGTGT LVHTSISFDL TLTTLFGPLL AGGQVVMLSE TAGVTGLIAA LRSRRDLTLV 730 740 750 760 770 780 KLTPTHLDVV NQLLTPDELR GAVRTLVVGG EAVRAESLEP FRASGTRVVN EYGPSETVVG 790 800 810 820 830 840 SVAHVVDAAT PRTGPVPIGR PIANTTVHLL DQRRRPVPDG VVGELWIGGA GVADGYLGRP 850 860 870 880 890 900 ELTGERFLPS DYPPDGGRVY RTGDLARRRA DGTLEYLGRT DAQVKIRGVR VEPAETEAVL 910 920 930 940 950 960 ASHPGVGQAV VVARLDEDPG RSSPLAGELT LTGYVVPARG AQAPPHEELI AYCRERLPEH 970 980 990 1000 1010 1020 FVPAVLVTLD ALPVTGHGKI DRGALPKPHA RARDGAAYVA PRTATEEILA ATVAKVLGVE 1030 1040 1050 1060 1070 1080 RVGIDDNYFV LGGDSIRSVM VASRAQARGV EVTVADLHRH PTVRACAAHL DAREDLPRTP 1090 1100 1110 1120 1130 1140 VTEPFALISA EDRALVPDDV EDAFPLNLLQ EGMIFHRDFA AKSAVYHAIA SVRLRAPFDL 1150 1160 1170 1180 1190 1200 AVLRMVVRQL VERHPMLRTS FDMSRFSRPL QLVHREFADP LHYEDLRGRS AEEQDARVEE 1210 1220 1230 1240 1250 1260 WIEREKERGF ELHEFPLIRF MAQRLEDDVF QFTYGFHHEI VDGWSEALMI TELFSHYFSV 1270 1280 1290 1300 1310 1320 IYDEPIAIKP PTAGMRDAVA LELEALADRR NYEFWDSYLA DATLMRLPRP GTGPRADKGD 1330 1340 1350 1360 1370 1380 RDITRIAVPV PTELSDGLKR VAATHAVPLK TVLLAAHMVV MSLYGGHEDT LTYTVTNGRP 1390 1400 1410 1420 1430 1440 ETADGSTAIG LFVNSLALRV RMTGGTWADL ITATLESERA SMPYRRLPMA ELKRHQGNEP 1450 1460 1470 1480 1490 1500 LAETLFFFTN YHVFHVLDRW IDRGVGHVAN ELYGESTFPF CGIFRLNRET GELEVRIEYD 1510 1520 1530 1540 1550 1560 SLQFSDALME SVRDSYARVL AALVADPDGR YDRHEFRSDR DRAALAVLTR GPEAPAADRC 1570 1580 1590 1600 1610 1620 LHDLVADRAA DRPDAPAVQL DTDVLSYGEL DRRANRLAHH LRSLGIGPES VVGVLAERSL 1630 1640 1650 1660 1670 1680 AQIIGLLAVL KAGAAYVPLD PAQPDERLAA VIAGSGAAAV LHRPGLEGRL PAGVRALPTD 1690 1700 1710 1720 1730 1740 AADGSTATHD PGPTATPRNA AYVMYTSGST GEPKGIVVEH RNVVASLAAR GAHYAAGPGR 1750 1760 1770 1780 1790 1800 FLLLSSFAFD SSVAGIFWTL TQGGTLVLPG EGQQLDPAAL VETIARQRPT HTLAIPSLLA 1810 1820 1830 1840 1850 1860 PVLDQAAPGD LASLRTVIAA GESCPAELAA ACRDLLPGST FHNEYGPTET TVWSTVWSQE 1870 1880 1890 1900 1910 1920 NEHDGPHLPI GRPVAGTWVH PRDHRGRTVP LGVAGELSIG GAGVARGYLG RPRDTAAAFR 1930 1940 1950 1960 1970 1980 PDPEATAPGG RAYATGDLGR YLPDGNLEFL GRADHQVKIR GFRVELGEIE AVLDTHPELQ 1990 2000 2010 2020 2030 2040 RTIVMARGDH PGDQVLVAYV LPAPGRRPEP ADIQGYVRDR LPRYMVPTAV IVLDAVPLTA 2050 2060 2070 2080 2090 2100 AGKVDRASLP APSHAQLTRD QEYVEPGTDT ERALAAIWAD VLKLDRIGAG DRFFDVGGES 2110 2120 2130 2140 2150 2160 LRAMQATAAA NKMFRTRVSV RRLFEAPSLR EFAHEIDKAR LAGGGTGLTG PAAAPATGGA AE

References

[1]	"An oxidation domain in the BlmIII non-ribosomal peptide synthetase probably catalyzing thiazole formation in the biosynthesis of the anti-tumor drug bleomycin in Streptomyces verticillus ATCC15003." Du L., Chen M., Sanchez C., Shen B. FEMS Microbiol. Lett. 189:171-175(2000) [PubMed: 10930733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15003 EMBL AAG02355.1.
[2]	"The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase." Du L., Sanchez C., Chen M., Edwards D.J., Shen B. Chem. Biol. 7:623-642(2000) [PubMed: 11048953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15003 EMBL AAG02355.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF210249 Genomic DNA. Translation: AAG02355.1.
3D structure databases
HSSP	HSSP built from PDB template 1AMU based on UniProtKB P14687.
ProteinModelPortal	Q9FB27.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR001242. Condensatn. IPR006163. Phsphopanteth-bd. IPR006162. PPantetheine_attach_site. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 2 hits.
Pfam	PF00501. AMP-binding. 2 hits. PF00668. Condensation. 2 hits. PF00550. PP-binding. 2 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 2 hits.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 2 hits. PS00455. AMP_BINDING. 1 hit. PS00012. PHOSPHOPANTETHEINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q9FB27_9ACTO

Accession

Primary (citable) accession number: Q9FB27

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2001
Last sequence update:	March 1, 2001
Last modified:	September 21, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)