Q9FB23 (Q9FB23_9ACTO) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Peptide synthetase NRPS5-4-3 EMBL AAG02359.1

Gene names

Name:

blmVI EMBL AAG02359.1

Organism

Streptomyces verticillus EMBL AAG02359.1

Taxonomic identifier

29309 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	2675 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Gene Ontology (GO)
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro ligase activity Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q9FB23 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 86B8D03DF511E7E7	FASTA	2,675	284,026
	10 20 30 40 50 60 MSRPAGIVDI ARRHAERTPA RPAYAFLPDG ETESVRFSFA DIDRRARAVA AVLQDRGLAG 70 80 90 100 110 120 ERVLVAYPSG PEYVQAFLGC LYAGVVAVPC DEPRSGPSAE RLAGIRADAR PALALTAGAP 130 140 150 160 170 180 EAGLAGLATL DVAGVPDSAA GAWTDPVAGP DALAFLQYTS GSTRRPRGVM VGHGNLLANE 190 200 210 220 230 240 RCIAAACGHD RDSTFVGWAP FFHDMGLVAN LLQPLYLGSL SVLMPPMAFL QRPARWLRAV 250 260 270 280 290 300 SRYRAHTSGG PNFAYDLCVD RVGEDERAGL DLSGWKVAYN GAEPVRADTL RRFTDRFAPH 310 320 330 340 350 360 GFTPGAHFPT YGLAEATLLV ATGPKGVPPR TLTADRAALR AGRLRPAGPG EAGLELVGNG 370 380 390 400 410 420 TAGLDTTLRI VDPATARECP PGEVGEVWVR GPGVARGYFG RPRESAPLLA ARLPGGEGPY 430 440 450 460 470 480 LRTGDLGALH DGELFLTGRH KDLIVIRGQN HHPHDLERTA EQAHPALRPT CAAAFAVPGD 490 500 510 520 530 540 GAERLVLVCE LTSYRAVDPA AVAEAVRAAL AARHGVAPHT LVVLRRGGIP KTTSGKVRRG 550 560 570 580 590 600 HCRTAYLDGT LPVHTAVRLP AGEEGTEALP LTTDPGRLAT ALRDLAAAHA GLAGPLPGTD 610 620 630 640 650 660 EPVSALGLDS LASLRLHHHV QSAYGVTLPV TALLGDTTYR RLAELTLAAP RPARAPEGQV 670 680 690 700 710 720 TGVWRPLTHG QRALWYEQAL APHAAAYHLV RALALRGPVD EEALAEAVRR VVRRHPALRT 730 740 750 760 770 780 RFALRDGEPA RRTEPYGPEL DVRDATGLPA DRLREHLAAA GDRPFDLAAG DRPVRLTLYR 790 800 810 820 830 840 TDGGHILLLV AHHLVADFWS LVVLLGDLAR AHAGEDLPPA PEGDPGDEAT DADRTYWRHR 850 860 870 880 890 900 LADAPPALDL PTDLPHPAER GFAGATHAFR LPPDLTARLT ALSRERHCTL FTTLLAAHQL 910 920 930 940 950 960 LLHRLTGQDD LVVGTLLARR DTAEAAGAVG YLVNPLPLRS VREPGETFTE LLRRTRRTVL 970 980 990 1000 1010 1020 DAVAHGRHPF GPLVSRLAPA RTPGRAPLLQ SLFVLQREYG DEADGYRALA LGVGGRLRVG 1030 1040 1050 1060 1070 1080 GLDLEALALP RRWSQLDLSL SMARLGDGLT GVWEYRTDLF TEATVAELSE AFVHLLRAAV 1090 1100 1110 1120 1130 1140 EDPGAPVETL PLTGGRETGP RRGPSAARPA LPLHRLVAAA ARRDPARTAV VALAPDGTAH 1150 1160 1170 1180 1190 1200 HISHGALHRA ATTLAARLRR EGAGPERPVA VLVERGPWLP VAYLGILHAG ATVLPLDPED 1210 1220 1230 1240 1250 1260 PPHRLARTIA NSGARLLLTE TGTASRAAEA AGPGVRALTV REGATGGERF SADVHPEQSA 1270 1280 1290 1300 1310 1320 YLLYTSGSTG DPKGVLVPHR AIVNRLLWMQ ETYRLRPGER VLHKTPVTFD VSMWELLWPL 1330 1340 1350 1360 1370 1380 TAGATVVMAR PGTHRDPARL VRRIAREAVT TVHFVPSMLT PFLTELARGT TRLPALRRVV 1390 1400 1410 1420 1430 1440 CSGEELPAAA VNRAAGLLDA RLYNLYGPTE AAVDVTAWPC RPPEPGPVPI GLPIANTTTE 1450 1460 1470 1480 1490 1500 VLDGRLRPLP RPVPGELYLG GACLAHGYHH DPALTAARFL PAPGGGRRYR TGDLVRQRAD 1510 1520 1530 1540 1550 1560 GALVFRGRTD DQVKIGGIRV EPGEVAEALR ALPGVADAAV VPHDGRLAAY AVADPVGPAP 1570 1580 1590 1600 1610 1620 AADALRDALR RRLPGHLVPA ALTLLDRLPL TPAGKLDRRA LPHPSAPPPD GGRPPTTGTE 1630 1640 1650 1660 1670 1680 RLVARVWAER LGREVVGVDR DFFSLGGDSV RALGVTAALR AAGLPVTVTD LLRLPTVAAL 1690 1700 1710 1720 1730 1740 ARHADERADR RPARQETPPG PFALCPEAAG VPGLEDAYPM SMAQRAVLFH RDHNPGYEVY 1750 1760 1770 1780 1790 1800 VTSVAVSTPL DRTRLAAAVD RLLDRHAYLR SSFDLVSHPE PTQLVWTHLP TPLEVVESSD 1810 1820 1830 1840 1850 1860 PAGFDAWLHA ERKRPLDVGT GPLARFTAHD AGAAGFRLTV SSFALDGWCV ATVLTELLRD 1870 1880 1890 1900 1910 1920 YWSALRGAPL SLPAPAASYR EFVALERAAQ HDPAHREFWR TELAGARPHP LPRRPVPPPG 1930 1940 1950 1960 1970 1980 PDGIRQHRHV VPVEDTVAKG LSALAGELGV GLKHVLLGVH LRVVRALSGD PDVITAVETH 1990 2000 2010 2020 2030 2040 GRLERHDGDR VLGVFNNILP LRQRVDGGSW ADLARAAHAA EARTGEYRRY PLAQAQRDHG 2050 2060 2070 2080 2090 2100 AAGLFDTLFV FTHFHLYRAL ADLDGMAVSD LRAPDQTYVP LTAHFNVDAT DGGGLRLLLE 2110 2120 2130 2140 2150 2160 SDPREFPDEQ VAEFAAYYRR ALRAAADAPH RPYRDTPLTD RPAGPAPHRA ERSVHALFAA 2170 2180 2190 2200 2210 2220 PARNHPDRIA LDGEDGPVSH GALARRAARL AGTLRAAGAG PDTVVGIWAP RRADAVVALL 2230 2240 2250 2260 2270 2280 AALHAGAAYL PLDPVHPPRR QRQVLTEAGA RLLVLPAGLD TPLRACGLPV VAPDDLGAPI 2290 2300 2310 2320 2330 2340 APVSVHPEQL AAVMATSGST GTPKTIGVPQ RALAGYLRWA IGHYRLDEET VSPVHSSLGF 2350 2360 2370 2380 2390 2400 DLTVTALLAP LAAGGQARLT DSGDPGALGA ALAAGHHTLL KITPAHLAAL AHQLGAPTAL 2410 2420 2430 2440 2450 2460 RTVVAGGEPL HAGHVRALRA FAPGARLVNE YGPTETTVGC CAHDVAPDPG EAPIPVGTPI 2470 2480 2490 2500 2510 2520 AGLSACVVDD ALPAPPGVRG ELYIGGTGVT RGYLGRPAAT AAAYVPDPAA PGARRYRTGD 2530 2540 2550 2560 2570 2580 LARRLPDGTL LLAGRADRQV KIRGHRVEPG EVEQVLGGHP GVREAAVVAH PAPGGGRRLV 2590 2600 2610 2620 2630 2640 AYWVPAEPAR PPSADALTAL LADRLPPYAV PAELVRLPAL PTTPNGKVDH TRLPAAGRDR 2650 2660 2670 RLAELLDRIE ALSDAEAASA LRDSRPAPGS GDDRA

References

[1]	"An oxidation domain in the BlmIII non-ribosomal peptide synthetase probably catalyzing thiazole formation in the biosynthesis of the anti-tumor drug bleomycin in Streptomyces verticillus ATCC15003." Du L., Chen M., Sanchez C., Shen B. FEMS Microbiol. Lett. 189:171-175(2000) [PubMed: 10930733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15003 EMBL AAG02359.1.
[2]	"The biosynthetic gene cluster for the antitumor drug bleomycin from Streptomyces verticillus ATCC15003 supporting functional interactions between nonribosomal peptide synthetases and a polyketide synthase." Du L., Sanchez C., Chen M., Edwards D.J., Shen B. Chem. Biol. 7:623-642(2000) [PubMed: 11048953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC15003 EMBL AAG02359.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF210249 Genomic DNA. Translation: AAG02359.1.
3D structure databases
HSSP	HSSP built from PDB template 1AMU based on UniProtKB P14687.
ProteinModelPortal	Q9FB23.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010071. AA_adenyl_domain. IPR009081. Acyl_carrier_prot-like. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR001242. Condensatn. IPR006163. Phsphopanteth-bd. IPR006162. PPantetheine_attach_site. [Graphical view]
Gene3D	G3DSA:1.10.1200.10. ACP_like. 2 hits.
Pfam	PF00501. AMP-binding. 3 hits. PF00668. Condensation. 2 hits. PF00550. PP-binding. 2 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 2 hits.
TIGRFAMs	TIGR01733. AA-adenyl-dom. 2 hits.
PROSITE	PS50075. ACP_DOMAIN. 2 hits. PS00455. AMP_BINDING. 2 hits. PS00012. PHOSPHOPANTETHEINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q9FB23_9ACTO

Accession

Primary (citable) accession number: Q9FB23

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2001
Last sequence update:	March 1, 2001
Last modified:	September 21, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)