Q9FAW5 (AZRB_BACOY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH azoreductase
EC=1.7.1.6

Gene names

Name:

azr

Organism

Bacillus sp. (strain OY1-2)

Taxonomic identifier

104667 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH as an electron donor for its activity. Compounds with paired naphthalene groups coupled with the azo group are good substrates, with the following preference order: Rocceline > Sumifix Black B > Solar Orange.
Catalytic activity	N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+ = 4-(dimethylamino)azobenzene + 2 NADPH.
Subunit structure	Monomer.
Induction	Constitutively expressed.
Sequence similarities	Belongs to the azoreductase type 2 family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 70 degrees Celsius.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	azobenzene reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	NADPH azoreductase		PRO_0000234085
Regions
	Nucleotide binding	106 – 111	6	NADP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9FAW5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: ADAF53CF849ABC59	FASTA	178	19,293
	10 20 30 40 50 60 MKLVVINGTP RKFGRTRVVA KYIADQFEGE LYDLAIEELP LYNGEESQRD LEAVKKLKTL 70 80 90 100 110 120 VKAADGVVLC TPEYHNAMSG ALKNSLDYLS SSEFIHKPVA LLAVAGGGKG GINALNSMHA 130 140 150 160 170 SLAGVYANAI PKQVVLDGLH VQDGELGEDA KPLIHDVVKE LKAYMSVYKE VKKQLGVE

References

[1]

"Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil." Suzuki Y., Yoda T., Ruhul A., Sugiura W. J. Biol. Chem. 276:9059-9065(2001) [PubMed: 11134015] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-31, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB032601 Genomic DNA. Translation: BAB13746.1.
3D structure databases
ProteinModelPortal	Q9FAW5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005025. FMN_red. [Graphical view]
Pfam	PF03358. FMN_red. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB01014. Balsalazide.

Entry information

Entry name

AZRB_BACOY

Accession

Primary (citable) accession number: Q9FAW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	March 1, 2001
Last modified:	September 21, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program