Reviewed, UniProtKB/Swiss-Prot Q9F9U5 (MHPB2_PSEPU)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 2
    EC=1.13.11.16

Gene names

Name:	mhpB2
Synonyms:	cbzE2

Encoded on

Plasmid pHMT112 Ref.1
Plasmid pKW1 Ref.2

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 2 HAMAP MF_01653		PRO_0000337663
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	20	1	N → T in AAX50134. Ref.1
	Sequence conflict	70	1	E → A in AAX50134. Ref.1
	Sequence conflict	81	1	A → V in AAX50134. Ref.1
	Sequence conflict	89 – 90	2	DV → EL in AAX50134. Ref.1
	Sequence conflict	122	1	D → E in AAX50134. Ref.1
	Sequence conflict	156	1	D → E in AAX50134. Ref.1
	Sequence conflict	203	1	N → D in AAX50134. Ref.1
	Sequence conflict	215	1	R → Q in AAX50134. Ref.1
	Sequence conflict	226	1	N → D in AAX50134. Ref.1
	Sequence conflict	239	1	R → Q in AAX50134. Ref.1
	Sequence conflict	250	1	L → V in AAX50134. Ref.1
	Sequence conflict	259	1	A → S in AAX50134. Ref.1
	Sequence conflict	283	1	S → A in AAX50134. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9F9U5-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F1B8670FF5D5BD50	FASTA	314	34,581
	10 20 30 40 50 60 MNAYLHCLSH TPLIGHFDPN QDVLDEVAEV VRAARARIEA FNPELVVLFA PDHYNGFFYD 70 80 90 100 110 120 VMPPFCLGME AEAIGDFGSL AGTLSVPKDV AEACAESVLT SGIDLAVSYR MQVDHGFAQP 130 140 150 160 170 180 LDFLLGGLDK YPVLPVFVNC VAPPLPTFER VRLLGDAIGR FTRGLNKRVL FLGSGGLSHQ 190 200 210 220 230 240 PPVPELAKVD ARMADRLMGS GRNLPPEERD ARTQRVVVAA ERFVENQNTL HPLNPKWDRY 250 260 270 280 290 300 FLDVVEQDLL SQLDDLSNAH LSELAGKSTH EVKAWVAAFS ALSAHGAYTA TDRYYRPIPE 310 WIAGFGSISA HTQR

References

[1]	"mhpB from Pseudomonas putida ML2." Panicker G., Tan H.M. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ML2.
[2]	"GJ31 meta-operon." Reineke W., Kunze M. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: GJ31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF176355 Genomic DNA. Translation: AAG09232.1. AY831461 Genomic DNA. Translation: AAX50134.1. Different initiation.
3D structure databases
SMR	Q9F9U5. Positions 7-309.
ModBase	Search...
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB2_PSEPU

Accession

Primary (citable) accession number: Q9F9U5
Secondary accession number(s): Q49KF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)