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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-315-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:CT0299
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
ProteomesiUP000001007 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503Glutamate--tRNA ligasePRO_0000119541Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT0299.

Structurei

3D structure databases

ProteinModelPortaliQ9F724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi259 – 2635"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9F724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGQKVRTRF APSPTGYLHV GGLRTALYNY LFAKRMNGDF VIRIEDTDQS
60 70 80 90 100
RKVEDAEKKL ISTLEWAGII ADESPMHGGN YGPYVQSQRL SIYRDYCTRL
110 120 130 140 150
LEDKNAYYCF STPEELEENR QLQLKQGLQP KYNRKWLPED MGGNMPESEI
160 170 180 190 200
KKKLDEGAPY VVRMKVPDYV SVWFEDMIRG PIEFDSATID DQVLMKSDGF
210 220 230 240 250
PTYHFASVID DHLMEFTHII RGEEWLPSMP KHLLLYEFFG WEPPKFAHLP
260 270 280 290 300
LLLNPDRSKL SKRQGDVAVE DYMRKGYSSE AIVNFVALLG WNEGEGSEQE
310 320 330 340 350
VFSMEELISK FSLERVGKAG AVFNVEKLSW LEKQYIKTRP VEKIVGNIKP
360 370 380 390 400
VLQAKLAEFS PEMSVERITS DDYLAKVVEL MRERVNFEHE FVTFSSYFFF
410 420 430 440 450
EPESYEEEAV AKRWTPNVPP LLQEFADLLE ANDDFTAENI EAQLKAFVAP
460 470 480 490 500
KGLKPAVLIH PIRIAVSGVS FGPSLYHMLE VLGKEAVLRR IRRAIERIEV

PAA
Length:503
Mass (Da):57,810
Last modified:August 30, 2002 - v2
Checksum:i8A265B64E72F4D80
GO

Sequence cautioni

The sequence AAG12192.1 differs from that shown. Reason: Frameshift at position 317. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71545.1.
AF287480 Genomic DNA. Translation: AAG12192.1. Frameshift.
RefSeqiNP_661203.1. NC_002932.3.
WP_010931991.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM71545; AAM71545; CT0299.
GeneIDi1007958.
KEGGicte:CT0299.
PATRICi21398713. VBIChlTep116050_0290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE006470 Genomic DNA. Translation: AAM71545.1.
AF287480 Genomic DNA. Translation: AAG12192.1. Frameshift.
RefSeqiNP_661203.1. NC_002932.3.
WP_010931991.1. NC_002932.3.

3D structure databases

ProteinModelPortaliQ9F724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT0299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM71545; AAM71545; CT0299.
GeneIDi1007958.
KEGGicte:CT0299.
PATRICi21398713. VBIChlTep116050_0290.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiAFRCFCT.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-315-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS.
  2. "Molecular evidence for the early evolution of photosynthesis."
    Xiong J., Fischer W.M., Inoue K., Nakahara M., Bauer C.E.
    Science 289:1724-1730(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 249-503.
    Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS.

Entry informationi

Entry nameiSYE_CHLTE
AccessioniPrimary (citable) accession number: Q9F724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 30, 2002
Last modified: May 27, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.