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Protein

Light-independent protochlorophyllide reductase subunit B

Gene

bchB

Organism
Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (BchN-BchB) is the catalytic component of the complex.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation1
Active sitei292Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processBacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:G1FZE-2198-MONOMER
UniPathwayiUPA00671

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit BUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit BUniRule annotation
Short name:
LI-POR subunit BUniRule annotation
Gene namesi
Name:bchBUniRule annotation
Ordered Locus Names:CT2151
OrganismiChlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002197981 – 537Light-independent protochlorophyllide reductase subunit BAdd BLAST537

Proteomic databases

PRIDEiQ9F715

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB. Forms a heterotetramer of two BchB and two BchN subunits.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT2151

Structurei

Secondary structure

1537
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi490 – 496Combined sources7
Helixi501 – 518Combined sources18
Beta strandi521 – 523Combined sources3
Helixi525 – 536Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KRUNMR-A484-537[»]
ProteinModelPortaliQ9F715
SMRiQ9F715
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9F715

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni428 – 429Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ChlB/BchB/BchZ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EVY Bacteria
COG2710 LUCA
HOGENOMiHOG000032963
KOiK04039
OMAiIPCAVIS
OrthoDBiPOG091H1082

Family and domain databases

HAMAPiMF_00353 ChlB_BchB, 1 hit
InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR013580 P-CP/CP_red_C
IPR005969 Protochl_reductB
IPR016209 Protochlorophyllide_Rdtase
PfamiView protein in Pfam
PF00148 Oxidored_nitro, 1 hit
PF08369 PCP_red, 1 hit
PIRSFiPIRSF000163 PCP_ChlB, 1 hit
TIGRFAMsiTIGR01278 DPOR_BchB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9F715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAFWLYEG TALHGVSRVT NSMKGVHTVY HAPQGDDYIT ATYTMLERTP
60 70 80 90 100
EFPKLSISVV RGQDLARGTS RLPGTVEQVD KHYKPELIVV APSCSTALLQ
110 120 130 140 150
EDLGQMARAS GVDQSKIMVY AVNPFRVAEN EAAEGLFTEL VRRFAAEQPK
160 170 180 190 200
TEKPSVNLLG FTSLGFHLRS NLTSLRRMLK TLGIEVNVVA PWGAGIDDLK
210 220 230 240 250
KLPAAWVNIA PFREIGCQAA GYLKEKFGMP SITEAPLGVN ATLRWLRAII
260 270 280 290 300
AEVNKIGAEK GMAPMAMPEL RDFSLDGQSA PSSVPWFART ADMESFSNKR
310 320 330 340 350
AFVFGDATQV VGVTKFLKDE LGMKIIGAGT YLPKQADWVR EQLEGYLPGE
360 370 380 390 400
LMVTDKFQEV SAFIEEEMPE LVCGTQMERH SCRKLDVPCM VISAPTHIEN
410 420 430 440 450
HLLGYYPFFG FDGADVMADR VYTSAKLGLE KHLIDFFGDA GLEYEAEEPE
460 470 480 490 500
AFTEPTMSGN GTVASVSSAE APSEAAVVTA TATGELSWTA EAEKMLGKVP
510 520 530
FFVRKKVRKN TDNYAREIGE PVVTADVFRK AKEHLGG
Length:537
Mass (Da):58,969
Last modified:March 1, 2001 - v1
Checksum:i4DD50F936A9836C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287482 Genomic DNA Translation: AAG12202.1
AE006470 Genomic DNA Translation: AAM73367.1
RefSeqiNP_663025.1, NC_002932.3
WP_010933804.1, NC_002932.3

Genome annotation databases

EnsemblBacteriaiAAM73367; AAM73367; CT2151
GeneIDi1007408
KEGGicte:CT2151
PATRICifig|194439.7.peg.1951

Similar proteinsi

Entry informationi

Entry nameiBCHB_CHLTE
AccessioniPrimary (citable) accession number: Q9F715
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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