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Protein

Light-independent protochlorophyllide reductase subunit B

Gene

bchB

Organism
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the dark-operative protochlorophyllide reductase (DPOR) that uses Mg-ATP and reduced ferredoxin to reduce ring D of protochlorophyllide (Pchlide) to form chlorophyllide a (Chlide). This reaction is light-independent. The NB-protein (BchN-BchB) is the catalytic component of the complex.UniRule annotation

Catalytic activityi

Protochlorophyllide a + reduced ferredoxin + 2 ATP + 2 H2O = chlorophyllide a + oxidized ferredoxin + 2 ADP + 2 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per heterodimer. The cluster is bound at the heterodimer interface by residues from both subunits.UniRule annotation

Pathwayi: bacteriochlorophyll biosynthesis (light-independent)

This protein is involved in the pathway bacteriochlorophyll biosynthesis (light-independent), which is part of Porphyrin-containing compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway bacteriochlorophyll biosynthesis (light-independent) and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Iron-sulfur (4Fe-4S); shared with heterodimeric partnerUniRule annotation
Active sitei292 – 2921Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bacteriochlorophyll biosynthesis, Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCTEP194439:GHN0-2198-MONOMER.
UniPathwayiUPA00671.

Names & Taxonomyi

Protein namesi
Recommended name:
Light-independent protochlorophyllide reductase subunit BUniRule annotation (EC:1.3.7.7UniRule annotation)
Short name:
DPOR subunit BUniRule annotation
Short name:
LI-POR subunit BUniRule annotation
Gene namesi
Name:bchBUniRule annotation
Ordered Locus Names:CT2151
OrganismiChlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
Taxonomic identifieri194439 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobaculum
Proteomesi
  • UP000001007 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Light-independent protochlorophyllide reductase subunit BPRO_0000219798Add
BLAST

Interactioni

Subunit structurei

Protochlorophyllide reductase is composed of three subunits; BchL, BchN and BchB. Forms a heterotetramer of two BchB and two BchN subunits.UniRule annotation

Protein-protein interaction databases

STRINGi194439.CT2151.

Structurei

Secondary structure

1
537
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi490 – 4967Combined sources
Helixi501 – 51818Combined sources
Beta strandi521 – 5233Combined sources
Helixi525 – 53612Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KRUNMR-A484-537[»]
ProteinModelPortaliQ9F715.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9F715.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni428 – 4292Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ChlB/BchB/BchZ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EVY. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000032963.
KOiK04039.
OMAiIPCAVIS.
OrthoDBiEOG6WX4K1.

Family and domain databases

HAMAPiMF_00353. ChlB_BchB.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR013580. P-CP/CP_red_C.
IPR005969. Protochl_reductB.
IPR016209. Protochlorophyllide_Rdtase.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
PF08369. PCP_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000163. PCP_ChlB. 1 hit.
TIGRFAMsiTIGR01278. DPOR_BchB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9F715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLAFWLYEG TALHGVSRVT NSMKGVHTVY HAPQGDDYIT ATYTMLERTP
60 70 80 90 100
EFPKLSISVV RGQDLARGTS RLPGTVEQVD KHYKPELIVV APSCSTALLQ
110 120 130 140 150
EDLGQMARAS GVDQSKIMVY AVNPFRVAEN EAAEGLFTEL VRRFAAEQPK
160 170 180 190 200
TEKPSVNLLG FTSLGFHLRS NLTSLRRMLK TLGIEVNVVA PWGAGIDDLK
210 220 230 240 250
KLPAAWVNIA PFREIGCQAA GYLKEKFGMP SITEAPLGVN ATLRWLRAII
260 270 280 290 300
AEVNKIGAEK GMAPMAMPEL RDFSLDGQSA PSSVPWFART ADMESFSNKR
310 320 330 340 350
AFVFGDATQV VGVTKFLKDE LGMKIIGAGT YLPKQADWVR EQLEGYLPGE
360 370 380 390 400
LMVTDKFQEV SAFIEEEMPE LVCGTQMERH SCRKLDVPCM VISAPTHIEN
410 420 430 440 450
HLLGYYPFFG FDGADVMADR VYTSAKLGLE KHLIDFFGDA GLEYEAEEPE
460 470 480 490 500
AFTEPTMSGN GTVASVSSAE APSEAAVVTA TATGELSWTA EAEKMLGKVP
510 520 530
FFVRKKVRKN TDNYAREIGE PVVTADVFRK AKEHLGG
Length:537
Mass (Da):58,969
Last modified:March 1, 2001 - v1
Checksum:i4DD50F936A9836C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287482 Genomic DNA. Translation: AAG12202.1.
AE006470 Genomic DNA. Translation: AAM73367.1.
RefSeqiNP_663025.1. NC_002932.3.
WP_010933804.1. NC_002932.3.

Genome annotation databases

EnsemblBacteriaiAAM73367; AAM73367; CT2151.
GeneIDi1007408.
KEGGicte:CT2151.
PATRICi21402109. VBIChlTep116050_1951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF287482 Genomic DNA. Translation: AAG12202.1.
AE006470 Genomic DNA. Translation: AAM73367.1.
RefSeqiNP_663025.1. NC_002932.3.
WP_010933804.1. NC_002932.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KRUNMR-A484-537[»]
ProteinModelPortaliQ9F715.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi194439.CT2151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM73367; AAM73367; CT2151.
GeneIDi1007408.
KEGGicte:CT2151.
PATRICi21402109. VBIChlTep116050_1951.

Phylogenomic databases

eggNOGiENOG4105EVY. Bacteria.
COG2710. LUCA.
HOGENOMiHOG000032963.
KOiK04039.
OMAiIPCAVIS.
OrthoDBiEOG6WX4K1.

Enzyme and pathway databases

UniPathwayiUPA00671.
BioCyciCTEP194439:GHN0-2198-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9F715.

Family and domain databases

HAMAPiMF_00353. ChlB_BchB.
InterProiIPR000510. Nase/OxRdtase_comp1.
IPR013580. P-CP/CP_red_C.
IPR005969. Protochl_reductB.
IPR016209. Protochlorophyllide_Rdtase.
[Graphical view]
PfamiPF00148. Oxidored_nitro. 1 hit.
PF08369. PCP_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000163. PCP_ChlB. 1 hit.
TIGRFAMsiTIGR01278. DPOR_BchB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular evidence for the early evolution of photosynthesis."
    Xiong J., Fischer W.M., Inoue K., Nakahara M., Bauer C.E.
    Science 289:1724-1730(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49652 / DSM 12025 / NBRC 103806 / TLS.

Entry informationi

Entry nameiBCHB_CHLTE
AccessioniPrimary (citable) accession number: Q9F715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.