Q9F5P4 (DNAB_MYCIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Replicative DNA helicase
EC=3.6.4.12

Cleaved into the following chain:

1. Min DnaB intein

Gene names

Name:

dnaB

Organism

Mycobacterium intracellulare

Taxonomic identifier

1767 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	576 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential.
Sequence similarities	Belongs to the helicase family. DnaB subfamily. Contains 1 DOD-type homing endonuclease domain. Contains 1 SF4 helicase domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Primosome
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	DNA duplex unwinding Inferred from electronic annotation. Source: GOC DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular_component	replisome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA helicase activity Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 15	›15	Replicative DNA helicase, 1st part Potential		PRO_0000013273
	Chain	16 – 351	336	Min DnaB intein Potential		PRO_0000013274
	Chain	352 – 576	225	Replicative DNA helicase, 2nd part Potential		PRO_0000013275
Regions
	Domain	154 – 260	107	DOD-type homing endonuclease
	Domain	313 – 576	264	SF4 helicase
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9F5P4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6D59341A779D082F	FASTA	576	63,530
	10 20 30 40 50 60 RWADIIVAAR PGVGKALALD TPLPTPTGWT TTGDVAVGDE LLGADGKPTR VVAATEVLLG 70 80 90 100 110 120 RPCYEVEFSD GTVIVADAAH QWLTETRASR KSAQAAAAGY NRYKNQRTFA AVRTTAEIAG 130 140 150 160 170 180 TLRCPTLDRR LNHSVVNARA LDLPDREFLV PPYTLGAWLG DGTSAAAQIT AADPEIIMRI 190 200 210 220 230 240 EAEMSSTVGT LQARLRTIGV LGNKHIPTEY LRGSETQRRE LLAGLLDTDG TVTAGGAVQF 250 260 270 280 290 300 SVTNQRLACD VAELIVSLGY RCQTSTKRVR GRNESSSIAY TLTFSTEKKM FALERKAIHK 310 320 330 340 350 360 ERRAMAGTTR SGSRFIVDVR PVETVAVRCV EVDNGSHLYL ASRAMVPTHN STLGLDFLRS 370 380 390 400 410 420 CSIKHRMASV IFSLEMSKSE IVMRLLSAEA KIKLADMRSG RMSDEDWTRL ARRMSEISEA 430 440 450 460 470 480 PLYIDDSPNL TMMEIRAKAR RLRQKADLRL VVVDYLQLMS SGKKVESRQL EVSEFSRQLK 490 500 510 520 530 540 LLAKELEVPV VAISQLNRGP EQRTDKKPML SDLRESGSLE QDADMVILLN RPDAFERDDP 550 560 570 RGGEADFILA KHRNGPTKTV TVAHQLHLSR FANMAR

References

[1]	Madiraju M.V.V.S., Yamamoto K., Rajagopalan M., Rutherford S.A. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1442.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF307984 Genomic DNA. Translation: AAG31144.1.
3D structure databases
ProteinModelPortal	Q9F5P4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR007694. DNA_helicase_DnaB-like_C. IPR006142. INTEIN. IPR004042. Intein_endonuc. [Graphical view]
Pfam	PF03796. DnaB_C. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN.
PROSITE	PS50818. INTEIN_C_TER. False negative. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. False negative. PS51199. SF4_HELICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAB_MYCIT

Accession

Primary (citable) accession number: Q9F5P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	February 6, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program