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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Mycobacterium intracellulare
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. endonuclease activity Source: InterPro

GO - Biological processi

  1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicase (EC:3.6.4.12)
Cleaved into the following chain:
Gene namesi
Name:dnaB
OrganismiMycobacterium intracellulare
Taxonomic identifieri1767 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Subcellular locationi

GO - Cellular componenti

  1. primosome complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 15›15Replicative DNA helicase, 1st partSequence AnalysisPRO_0000013273Add
BLAST
Chaini16 – 351336Min DnaB inteinSequence AnalysisPRO_0000013274Add
BLAST
Chaini352 – 576225Replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013275Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliQ9F5P4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini154 – 260107DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST
Domaini313 – 576264SF4 helicasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR007694. DNA_helicase_DnaB-like_C.
IPR028992. Hedgehog/Intein_dom.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
PROSITEiPS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9F5P4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RWADIIVAAR PGVGKALALD TPLPTPTGWT TTGDVAVGDE LLGADGKPTR
60 70 80 90 100
VVAATEVLLG RPCYEVEFSD GTVIVADAAH QWLTETRASR KSAQAAAAGY
110 120 130 140 150
NRYKNQRTFA AVRTTAEIAG TLRCPTLDRR LNHSVVNARA LDLPDREFLV
160 170 180 190 200
PPYTLGAWLG DGTSAAAQIT AADPEIIMRI EAEMSSTVGT LQARLRTIGV
210 220 230 240 250
LGNKHIPTEY LRGSETQRRE LLAGLLDTDG TVTAGGAVQF SVTNQRLACD
260 270 280 290 300
VAELIVSLGY RCQTSTKRVR GRNESSSIAY TLTFSTEKKM FALERKAIHK
310 320 330 340 350
ERRAMAGTTR SGSRFIVDVR PVETVAVRCV EVDNGSHLYL ASRAMVPTHN
360 370 380 390 400
STLGLDFLRS CSIKHRMASV IFSLEMSKSE IVMRLLSAEA KIKLADMRSG
410 420 430 440 450
RMSDEDWTRL ARRMSEISEA PLYIDDSPNL TMMEIRAKAR RLRQKADLRL
460 470 480 490 500
VVVDYLQLMS SGKKVESRQL EVSEFSRQLK LLAKELEVPV VAISQLNRGP
510 520 530 540 550
EQRTDKKPML SDLRESGSLE QDADMVILLN RPDAFERDDP RGGEADFILA
560 570
KHRNGPTKTV TVAHQLHLSR FANMAR
Length:576
Mass (Da):63,530
Last modified:March 1, 2001 - v1
Checksum:i6D59341A779D082F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307984 Genomic DNA. Translation: AAG31144.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307984 Genomic DNA. Translation: AAG31144.1.

3D structure databases

ProteinModelPortaliQ9F5P4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR007694. DNA_helicase_DnaB-like_C.
IPR028992. Hedgehog/Intein_dom.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF03796. DnaB_C. 1 hit.
PF14528. LAGLIDADG_3. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF55608. SSF55608. 1 hit.
PROSITEiPS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS51199. SF4_HELICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Madiraju M.V.V.S., Yamamoto K., Rajagopalan M., Rutherford S.A.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 1442.

Entry informationi

Entry nameiDNAB_MYCIT
AccessioniPrimary (citable) accession number: Q9F5P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: October 1, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.