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Q9F5P4

- DNAB_MYCIT

UniProt

Q9F5P4 - DNAB_MYCIT

Protein

Replicative DNA helicase

Gene

dnaB

Organism
Mycobacterium intracellulare
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro
    4. endonuclease activity Source: InterPro

    GO - Biological processi

    1. DNA replication, synthesis of RNA primer Source: UniProtKB-KW
    2. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Replicative DNA helicase (EC:3.6.4.12)
    Cleaved into the following chain:
    Gene namesi
    Name:dnaB
    OrganismiMycobacterium intracellulare
    Taxonomic identifieri1767 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

    Subcellular locationi

    GO - Cellular componenti

    1. primosome complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Primosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 15›15Replicative DNA helicase, 1st partSequence AnalysisPRO_0000013273Add
    BLAST
    Chaini16 – 351336Min DnaB inteinSequence AnalysisPRO_0000013274Add
    BLAST
    Chaini352 – 576225Replicative DNA helicase, 2nd partSequence AnalysisPRO_0000013275Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliQ9F5P4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini154 – 260107DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST
    Domaini313 – 576264SF4 helicasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the helicase family. DnaB subfamily.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation
    Contains 1 SF4 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProiIPR007694. DNA_helicase_DnaB-like_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    PROSITEiPS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS51199. SF4_HELICASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9F5P4-1 [UniParc]FASTAAdd to Basket

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    RWADIIVAAR PGVGKALALD TPLPTPTGWT TTGDVAVGDE LLGADGKPTR    50
    VVAATEVLLG RPCYEVEFSD GTVIVADAAH QWLTETRASR KSAQAAAAGY 100
    NRYKNQRTFA AVRTTAEIAG TLRCPTLDRR LNHSVVNARA LDLPDREFLV 150
    PPYTLGAWLG DGTSAAAQIT AADPEIIMRI EAEMSSTVGT LQARLRTIGV 200
    LGNKHIPTEY LRGSETQRRE LLAGLLDTDG TVTAGGAVQF SVTNQRLACD 250
    VAELIVSLGY RCQTSTKRVR GRNESSSIAY TLTFSTEKKM FALERKAIHK 300
    ERRAMAGTTR SGSRFIVDVR PVETVAVRCV EVDNGSHLYL ASRAMVPTHN 350
    STLGLDFLRS CSIKHRMASV IFSLEMSKSE IVMRLLSAEA KIKLADMRSG 400
    RMSDEDWTRL ARRMSEISEA PLYIDDSPNL TMMEIRAKAR RLRQKADLRL 450
    VVVDYLQLMS SGKKVESRQL EVSEFSRQLK LLAKELEVPV VAISQLNRGP 500
    EQRTDKKPML SDLRESGSLE QDADMVILLN RPDAFERDDP RGGEADFILA 550
    KHRNGPTKTV TVAHQLHLSR FANMAR 576
    Length:576
    Mass (Da):63,530
    Last modified:March 1, 2001 - v1
    Checksum:i6D59341A779D082F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF307984 Genomic DNA. Translation: AAG31144.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF307984 Genomic DNA. Translation: AAG31144.1 .

    3D structure databases

    ProteinModelPortali Q9F5P4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 2 hits.
    3.40.50.300. 2 hits.
    InterProi IPR007694. DNA_helicase_DnaB-like_C.
    IPR028992. Hedgehog/Intein_dom.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF03796. DnaB_C. 1 hit.
    PF14528. LAGLIDADG_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF55608. SSF55608. 1 hit.
    PROSITEi PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS51199. SF4_HELICASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Madiraju M.V.V.S., Yamamoto K., Rajagopalan M., Rutherford S.A.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1442.

    Entry informationi

    Entry nameiDNAB_MYCIT
    AccessioniPrimary (citable) accession number: Q9F5P4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3