Q9F5P3 (DCEA_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate decarboxylase alpha
Short name=GAD-alpha
EC=4.1.1.15

Gene names

Name:	gadA
Ordered Locus Names:	lmo0447

Organism

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]

Taxonomic identifier

169963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Glutamate decarboxylase alpha		PRO_0000146988
Amino acid modifications
	Modified residue	273	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	152	1	S → N in AAG22560. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9F5P3 [UniParc]. Last modified February 21, 2002. Version 2. Checksum: EA1A442E3E1CE2FA	FASTA	462	52,498
	10 20 30 40 50 60 MFKTNVEQNN VPVFGSFESG QDLPEKRMNK ESVDPRIAYQ LVKDQLIDEG SARQNLATFC 70 80 90 100 110 120 QTYMEPEAEQ IMAETMEKNA IDKSEYPQTA KLESSCVNML ADLWNVDESE HYMGTSTVGS 130 140 150 160 170 180 SEACMLGGMA MKFRWRSAAL KNGLDIHAKK PSLVISSGYQ VCWEKFCVYW DIELREVPMS 190 200 210 220 230 240 EEHLSINTDI IMDYVDEYTI GIVGILGITY TGKFDDIMTL NDLVEDYNNT HDNEVVIHVD 250 260 270 280 290 300 GASGAMFTPF VEPGLEWDFR LPNVVSINTS GHKYGLVYPG VGWILWRDKE YLPEELVFDV 310 320 330 340 350 360 SYLGGHMPTM AINFSRSASQ IIGQYYNFLR FGYEGYRQIH MRTRDGALQL SQAVAETGLF 370 380 390 400 410 420 EIYNDGANLP IVCYKLKDDA NVAWTLYDLA DRLQMKGWQV PAYPLPKEMG NTIIQRYVCR 430 440 450 460 GDLGQNMVTA FKNDLSESIE ELNNAHILYH DVNTSKTHGF TH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid." Cotter P.D., Gahan C.G.M., Hill C. Mol. Microbiol. 40:465-475(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: LO28 / Serovar 1/2c.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF309076 Genomic DNA. Translation: AAG22560.1. AL591975 Genomic DNA. Translation: CAC98526.1.
PIR	AH1130.
RefSeq	NP_463976.1. NC_003210.1.
3D structure databases
ProteinModelPortal	Q9F5P3.
ModBase	Search...
Protein-protein interaction databases
STRING	169963.lmo0447.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC98526; CAC98526; CAC98526.
GeneID	986724.
KEGG	lmo:lmo0447.
PATRIC	20309953. VBILisMon69206_0462.
Organism-specific databases
GenoList	LMO0447.
Phylogenomic databases
eggNOG	COG0076.
HOGENOM	HOG000070228.
KO	K01580.
OMA	IELREVP.
ProtClustDB	CLSK876864.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCEA_LISMO

Accession

Primary (citable) accession number: Q9F5P3
Secondary accession number(s): Q8Y9S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program