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Protein

Glutamate decarboxylase alpha

Gene

gadA

Organism
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. glutamate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciLMON169963:LMO0447-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase alpha (EC:4.1.1.15)
Short name:
GAD-alpha
Gene namesi
Name:gadA
Ordered Locus Names:lmo0447
OrganismiListeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Taxonomic identifieri169963 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000000817 Componenti: Chromosome

Organism-specific databases

GenoListiLMO0447.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glutamate decarboxylase alphaPRO_0000146988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei273 – 2731N6-(pyridoxal phosphate)lysineBy similarity

Interactioni

Protein-protein interaction databases

STRINGi169963.lmo0447.

Structurei

3D structure databases

ProteinModelPortaliQ9F5P3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000070228.
KOiK01580.
OMAiDESEHYM.
OrthoDBiEOG618QPT.
PhylomeDBiQ9F5P3.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9F5P3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKTNVEQNN VPVFGSFESG QDLPEKRMNK ESVDPRIAYQ LVKDQLIDEG
60 70 80 90 100
SARQNLATFC QTYMEPEAEQ IMAETMEKNA IDKSEYPQTA KLESSCVNML
110 120 130 140 150
ADLWNVDESE HYMGTSTVGS SEACMLGGMA MKFRWRSAAL KNGLDIHAKK
160 170 180 190 200
PSLVISSGYQ VCWEKFCVYW DIELREVPMS EEHLSINTDI IMDYVDEYTI
210 220 230 240 250
GIVGILGITY TGKFDDIMTL NDLVEDYNNT HDNEVVIHVD GASGAMFTPF
260 270 280 290 300
VEPGLEWDFR LPNVVSINTS GHKYGLVYPG VGWILWRDKE YLPEELVFDV
310 320 330 340 350
SYLGGHMPTM AINFSRSASQ IIGQYYNFLR FGYEGYRQIH MRTRDGALQL
360 370 380 390 400
SQAVAETGLF EIYNDGANLP IVCYKLKDDA NVAWTLYDLA DRLQMKGWQV
410 420 430 440 450
PAYPLPKEMG NTIIQRYVCR GDLGQNMVTA FKNDLSESIE ELNNAHILYH
460
DVNTSKTHGF TH
Length:462
Mass (Da):52,498
Last modified:February 20, 2002 - v2
Checksum:iEA1A442E3E1CE2FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1521S → N in AAG22560 (PubMed:11309128).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309076 Genomic DNA. Translation: AAG22560.1.
AL591975 Genomic DNA. Translation: CAC98526.1.
PIRiAH1130.
RefSeqiNP_463976.1. NC_003210.1.
WP_010989470.1. NC_003210.1.

Genome annotation databases

GeneIDi986724.
KEGGilmo:lmo0447.
PATRICi20309953. VBILisMon69206_0462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF309076 Genomic DNA. Translation: AAG22560.1.
AL591975 Genomic DNA. Translation: CAC98526.1.
PIRiAH1130.
RefSeqiNP_463976.1. NC_003210.1.
WP_010989470.1. NC_003210.1.

3D structure databases

ProteinModelPortaliQ9F5P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi169963.lmo0447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi986724.
KEGGilmo:lmo0447.
PATRICi20309953. VBILisMon69206_0462.

Organism-specific databases

GenoListiLMO0447.

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000070228.
KOiK01580.
OMAiDESEHYM.
OrthoDBiEOG618QPT.
PhylomeDBiQ9F5P3.

Enzyme and pathway databases

BioCyciLMON169963:LMO0447-MONOMER.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid."
    Cotter P.D., Gahan C.G.M., Hill C.
    Mol. Microbiol. 40:465-475(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: LO28 / Serovar 1/2c.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-679 / EGD-e.

Entry informationi

Entry nameiDCEA_LISMO
AccessioniPrimary (citable) accession number: Q9F5P3
Secondary accession number(s): Q8Y9S6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2002
Last sequence update: February 20, 2002
Last modified: March 31, 2015
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.