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Protein

Iota-carrageenase

Gene

cgiA

Organism
Alteromonas fortis (Alteromonas sp. (strain ATCC 43554))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes iota-carrageenans, sulfated 1,3-alpha-1,4-beta galactans from red algal cell walls, with an inversion of anomeric configuration. Also active against hybrid iota-/nu-carrageenan, not active against kappa- or lambda-carrageenans.By similarity2 Publications

Catalytic activityi

Endohydrolysis of 1,4-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.157. 8122.

Protein family/group databases

CAZyiGH82. Glycoside Hydrolase Family 82.

Names & Taxonomyi

Protein namesi
Recommended name:
Iota-carrageenase (EC:3.2.1.157)
Gene namesi
Name:cgiAImported
OrganismiAlteromonas fortis (Alteromonas sp. (strain ATCC 43554))
Taxonomic identifieri116059 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas

Subcellular locationi

  • Secretedextracellular space By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_500006597724 – 491Iota-carrageenaseSequence analysisAdd BLAST468

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi269 ↔ 2982 Publications
Disulfide bondi336 ↔ 3602 Publications
Disulfide bondi408 ↔ 4762 Publications
Disulfide bondi412 ↔ 4842 Publications

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1491
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 37Combined sources3
Beta strandi46 – 50Combined sources5
Helixi51 – 55Combined sources5
Beta strandi61 – 64Combined sources4
Helixi66 – 77Combined sources12
Beta strandi83 – 87Combined sources5
Beta strandi89 – 94Combined sources6
Beta strandi96 – 98Combined sources3
Beta strandi103 – 107Combined sources5
Beta strandi112 – 115Combined sources4
Beta strandi124 – 133Combined sources10
Beta strandi135 – 142Combined sources8
Beta strandi147 – 150Combined sources4
Beta strandi158 – 164Combined sources7
Beta strandi166 – 178Combined sources13
Beta strandi186 – 189Combined sources4
Beta strandi191 – 194Combined sources4
Beta strandi197 – 213Combined sources17
Beta strandi219 – 245Combined sources27
Helixi249 – 254Combined sources6
Beta strandi258 – 279Combined sources22
Beta strandi288 – 299Combined sources12
Beta strandi301 – 304Combined sources4
Beta strandi310 – 312Combined sources3
Beta strandi315 – 317Combined sources3
Helixi321 – 332Combined sources12
Beta strandi344 – 348Combined sources5
Beta strandi352 – 354Combined sources3
Helixi358 – 367Combined sources10
Beta strandi376 – 386Combined sources11
Beta strandi391 – 393Combined sources3
Helixi395 – 402Combined sources8
Turni416 – 418Combined sources3
Beta strandi426 – 428Combined sources3
Beta strandi433 – 436Combined sources4
Beta strandi440 – 444Combined sources5
Beta strandi448 – 457Combined sources10
Beta strandi464 – 466Combined sources3
Turni478 – 480Combined sources3
Helixi486 – 489Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H80X-ray1.60A/B28-491[»]
1KTWX-ray2.00A/B28-491[»]
3LMWX-ray2.60A/B27-491[»]
ProteinModelPortaliQ9F5I8.
SMRiQ9F5I8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9F5I8.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 82 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR024535. Pectate_lyase_SF_prot.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF12708. Pectate_lyase_3. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9F5I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLYFRKLWL TNLFLGGALA SSAAIGAVSP KTYKDADFYV APTQQDVNYD
60 70 80 90 100
LVDDFGANGN DTSDDSNALQ RAINAISRKP NGGTLLIPNG TYHFLGIQMK
110 120 130 140 150
SNVHIRVESD VIIKPTWNGD GKNHRLFEVG VNNIVRNFSF QGLGNGFLVD
160 170 180 190 200
FKDSRDKNLA VFKLGDVRNY KISNFTIDDN KTIFASILVD VTERNGRLHW
210 220 230 240 250
SRNGIIERIK QNNALFGYGL IQTYGADNIL FRNLHSEGGI ALRMETDNLL
260 270 280 290 300
MKNYKQGGIR NIFADNIRCS KGLAAVMFGP HFMKNGDVQV TNVSSVSCGS
310 320 330 340 350
AVRSDSGFVE LFSPTDEVHT RQSWKQAVES KLGRGCAQTP YARGNGGTRW
360 370 380 390 400
AARVTQKDAC LDKAKLEYGI EPGSFGTVKV FDVTARFGYN ADLKQDQLDY
410 420 430 440 450
FSTSNPMCKR VCLPTKEQWS KQGQIYIGPS LAAVIDTTPE TSKYDYDVKT
460 470 480 490
FNVKRINFPV NSHKTIDTNT ESSRVCNYYG MSECSSSRWE R
Length:491
Mass (Da):54,795
Last modified:March 1, 2001 - v1
Checksum:iD5912275F06992CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272076 Genomic DNA. Translation: CAC07801.1.

Genome annotation databases

KEGGiag:CAC07801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272076 Genomic DNA. Translation: CAC07801.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H80X-ray1.60A/B28-491[»]
1KTWX-ray2.00A/B28-491[»]
3LMWX-ray2.60A/B27-491[»]
ProteinModelPortaliQ9F5I8.
SMRiQ9F5I8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH82. Glycoside Hydrolase Family 82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAC07801.

Enzyme and pathway databases

BRENDAi3.2.1.157. 8122.

Miscellaneous databases

EvolutionaryTraceiQ9F5I8.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR024535. Pectate_lyase_SF_prot.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF12708. Pectate_lyase_3. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCGIA_ALTFO
AccessioniPrimary (citable) accession number: Q9F5I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.