Reviewed, UniProtKB/Swiss-Prot Q9F415 (RECA_MYCFV)

Names and origin

Protein names

Recommended name:
    Protein recA
Alternative name(s):
    Recombinase A
Cleaved into the following chain:
    1- Recommended name:
            Mfl recA intein

Gene names

Name:

recA

Organism

Mycobacterium flavescens

Taxonomic identifier

1776 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	423 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. HAMAP MF_00268
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.
Sequence similarities	Belongs to the recA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 9	›9	Protein recA, 1st part Potential		PRO_0000030254
	Chain	10 – 373	364	Mfl recA intein Potential		PRO_0000030255
	Chain	374 – ›423	›50	Protein recA, 2nd part Potential		PRO_0000030256
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	423	1

Sequences

	Sequence		Length	Mass (Da)
	Q9F415-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8CF63171128DC0F4	FASTA	423	47,333
	10 20 30 40 50 60 REKIGVMFGC FNYSTRVQLA DRTTEKIGKI VTQKMDVEVL SYDPDTDRVV PRKVVNWFNN 70 80 90 100 110 120 GPAEQFLQFT VEKSGGNGKS QFAATPNHLI RTPGGWTEAG DLIAGDRVMA TEPHRLSDQQ 130 140 150 160 170 180 FQVVLGSLMG DGNLSPNRRD RNGVRFRMGH GAKQVDYLRW KTELLGNIKH STRVNDKGAT 190 200 210 220 230 240 FVDFTPLPEL AELQRAVYLG DGKKFLSEEY LKALTPLALA IWYMDDGAFT VRSKGLQERT 250 260 270 280 290 300 AGGSGRIEIC VEAMSEGTRV RLRDYLHDTH GLEVRLRQSG KAGKAVLVFS TASSAKFQEL 310 320 330 340 350 360 VAPYMAPSME YKLLPRFRGQ STVSPQFVES TQRLVPARIL DVHVKPHTRS MNRFDIEVEG 370 380 390 400 410 420 NHNYFVDGVM VHNSPETTTG GKALKFYASV RMDVRRIETL KDGXDAVGNR TRVKVVKNKV SPP

References

[1]	"Inteins invading mycobacterial RecA proteins." Saves I., Laneelle M.-A., Daffe M., Masson J.-M. FEBS Lett. 480:221-225(2000) [PubMed: 11034333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14474 / DSM 43991 / NCTC 10271 / TMC 1541.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ251150 Genomic DNA. Translation: CAC09590.1.
3D structure databases
SMR	Q9F415. Positions 328-423.
ModBase	Search...
Family and domain databases
HAMAP	MF_00268. [Tree]
InterPro	IPR013765. DNA_recomb/repair_RecA. IPR001553. DNA_recomb/repair_RecA/RadB. IPR020584. DNA_recomb/repair_RecA_CS. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR006141. Intein_splicing_site. IPR004860. LAGLIDADG_2. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF1. RecA. 1 hit.
Pfam	PF03161. LAGLIDADG_2. 1 hit. PF00154. RecA. 1 hit. [Graphical view]
PRINTS	PR00379. INTEIN. PR00142. RECA.
SMART	SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00321. RECA_1. 1 hit. PS50162. RECA_2. False negative. PS50163. RECA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECA_MYCFV

Accession

Primary (citable) accession number: Q9F415

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	March 1, 2001
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)