Q9F411 (TRMB_MYCHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	MHO_4250

Organism

Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21) [Complete proteome] [HAMAP]

Taxonomic identifier

347256 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma hominis

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171352
Regions
	Region	181 – 184	4	Substrate binding Potential
Sites
	Binding site	34	1	S-adenosyl-L-methionine By similarity
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	86	1	S-adenosyl-L-methionine By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	143	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9F411 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D8993C44B381BB0B	FASTA	202	23,951
	10 20 30 40 50 60 MRLRFNKNAE TSLMASPMTF KDFPIDNKKN TILEIGMGRG TMLTKLALMH PDIEYIGLEK 70 80 90 100 110 120 YSTPAYSALK KAIDLNLENF HIIIGDAINL STYFKNKIKT IWLTFSDPWP KKRHYKRRLV 130 140 150 160 170 180 YRDFLKIYQN VLDKDGVVYF KTDNDMLYQF AIDELKEINA KIIYQTSDLH HCNFKIENVF 190 200 TDYEEKFNKL NKNINFIAFT FN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequence of the DNA gyrase (gyrA) gene from Mycoplasma hominis and characterization of quinolone-resistant mutants selected In vitro with trovafloxacin." Bebear C.M., Grau O., Charron A., Renaudin H., Gruson D., Bebear C. Antimicrob. Agents Chemother. 44:2719-2727(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Life on arginine for Mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas." Pereyre S., Sirand-Pugnet P., Beven L., Charron A., Renaudin H., Barre A., Avenaud P., Jacob D., Couloux A., Barbe V., de Daruvar A., Blanchard A., Bebear C. PLoS Genet. 5:E1000677-E1000677(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59880 Genomic DNA. Translation: AAG28841.1. FP236530 Genomic DNA. Translation: CAX37560.1.
RefSeq	YP_003302963.1. NC_013511.1.
3D structure databases
ProteinModelPortal	Q9F411.
ModBase	Search...
Protein-protein interaction databases
STRING	2098.MHO_4250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAX37560; CAX37560; MHO_4250.
GeneID	8595146.
KEGG	mho:MHO_4250.
PATRIC	32274019. VBIMycHom120329_0443.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000251689.
KO	K03439.
OMA	RLRNKPW.
ProtClustDB	PRK00121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_MYCHP

Accession

Primary (citable) accession number: Q9F411
Secondary accession number(s): D1J8L3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program