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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Mycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34S-adenosyl-L-methionineUniRule annotation1
Binding sitei59S-adenosyl-L-methionineUniRule annotation1
Binding sitei86S-adenosyl-L-methionineUniRule annotation1
Active sitei107By similarity1
Binding sitei107S-adenosyl-L-methionineUniRule annotation1
Binding sitei111SubstrateUniRule annotation1
Binding sitei143SubstrateUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:MHO_4250
OrganismiMycoplasma hominis (strain ATCC 23114 / NBRC 14850 / NCTC 10111 / PG21)
Taxonomic identifieri347256 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000002631 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713521 – 202tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST202

Interactioni

Protein-protein interaction databases

STRINGi347256.MHO_4250.

Structurei

3D structure databases

ProteinModelPortaliQ9F411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 184Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107T6Z. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9F411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRFNKNAE TSLMASPMTF KDFPIDNKKN TILEIGMGRG TMLTKLALMH
60 70 80 90 100
PDIEYIGLEK YSTPAYSALK KAIDLNLENF HIIIGDAINL STYFKNKIKT
110 120 130 140 150
IWLTFSDPWP KKRHYKRRLV YRDFLKIYQN VLDKDGVVYF KTDNDMLYQF
160 170 180 190 200
AIDELKEINA KIIYQTSDLH HCNFKIENVF TDYEEKFNKL NKNINFIAFT

FN
Length:202
Mass (Da):23,951
Last modified:March 1, 2001 - v1
Checksum:iD8993C44B381BB0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59880 Genomic DNA. Translation: AAG28841.1.
FP236530 Genomic DNA. Translation: CAX37560.1.
RefSeqiWP_012855699.1. NC_013511.1.

Genome annotation databases

EnsemblBacteriaiCAX37560; CAX37560; MHO_4250.
GeneIDi25040506.
KEGGimho:MHO_4250.
PATRICi32274019. VBIMycHom120329_0443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59880 Genomic DNA. Translation: AAG28841.1.
FP236530 Genomic DNA. Translation: CAX37560.1.
RefSeqiWP_012855699.1. NC_013511.1.

3D structure databases

ProteinModelPortaliQ9F411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347256.MHO_4250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX37560; CAX37560; MHO_4250.
GeneIDi25040506.
KEGGimho:MHO_4250.
PATRICi32274019. VBIMycHom120329_0443.

Phylogenomic databases

eggNOGiENOG4107T6Z. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000251689.
KOiK03439.
OMAiAHPEINY.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_MYCHP
AccessioniPrimary (citable) accession number: Q9F411
Secondary accession number(s): D1J8L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.