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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium shimoidei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity).By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA-dependent ATPase activity Source: InterPro
  3. endonuclease activity Source: InterPro
  4. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-KW
  2. DNA repair Source: UniProtKB-KW
  3. intein-mediated protein splicing Source: InterPro
  4. SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Gene namesi
Name:recA
OrganismiMycobacterium shimoidei
Taxonomic identifieri29313 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 9›9Protein RecA, 1st partSequence AnalysisPRO_0000030263
Chaini10 – 373364Msh RecA inteinSequence AnalysisPRO_0000030264Add
BLAST
Chaini374 – ›423›50Protein RecA, 2nd partSequence AnalysisPRO_0000030265Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.By similarity

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliQ9F410.
SMRiQ9F410. Positions 368-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RecA family.Curated

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR006141. Intein_splice_site.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF03161. LAGLIDADG_2. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9F410-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
REKIGVMFGC MNYSTRVTLA DGSTEKIGKI VNNKMDVKVL SYDPDSDRIV
60 70 80 90 100
PRKIVNWFNN GPAEQFLQFT VEKSGGNGKS QFAATPNHLI RTPAGWTEAG
110 120 130 140 150
DLNTGDRVLA AEPHLLSDQQ FQVVLGSLMG DGNLSPNRRD RNGVRFRLGH
160 170 180 190 200
GAKQAEYLQW KTALMGNIGH TVRENAKGAS FVDFTPLPEL AELQRAVYMG
210 220 230 240 250
DGKKFFSEEY LKALTPLALA IWYMDDGSFT LRSRGLQERT AGGSGRIAIC
260 270 280 290 300
VEAMTEGTRV RLRDYLRDTH GLDVRLRSAG SAGKTVLVFS MAATAKFQEL
310 320 330 340 350
VAPYMAPSME YKLLPRFRGR STVRPQFVEP TQRLVPARIL DVHVKPHTRS
360 370 380 390 400
MNRFDIEVEG NHNYFVDGVM VHNSPETTTG GKALKFYASV RMDVRRIETL
410 420
KDGTDAVGNR TRVKVVKNKV SPP
Length:423
Mass (Da):47,084
Last modified:March 1, 2001 - v1
Checksum:iD36A8C64C39F2AEA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei423 – 4231

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251338 Genomic DNA. Translation: CAC09592.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251338 Genomic DNA. Translation: CAC09592.1.

3D structure databases

ProteinModelPortaliQ9F410.
SMRiQ9F410. Positions 368-423.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR006141. Intein_splice_site.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF03161. LAGLIDADG_2. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27962 / DSM 44152 / JCM 12376.

Entry informationi

Entry nameiRECA_MYCSH
AccessioniPrimary (citable) accession number: Q9F410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.