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Q9F305 (TRMB_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SCO4111
ORF Names:SCD17A.03c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length271 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 271271tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171401

Regions

Region241 – 2444Substrate binding By similarity

Sites

Active site1681 By similarity
Binding site931S-adenosyl-L-methionine By similarity
Binding site1181S-adenosyl-L-methionine By similarity
Binding site1451S-adenosyl-L-methionine By similarity
Binding site1681S-adenosyl-L-methionine By similarity
Binding site1721Substrate By similarity
Binding site2041Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9F305 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 8E9A7A05BA6E2829

FASTA27129,912
        10         20         30         40         50         60 
MSDSHHTPEA ASASLRHVRA KGEPRFPDGP KADPAGSHFE RRIRSFQPRR SRVTAGQADA 

        70         80         90        100        110        120 
LQRLWPLWGL DIDGRRVVDL AELFGNARPV VLEIGFGMGE TTARMAAADP DTNILAADVH 

       130        140        150        160        170        180 
TPGQGNLLGL AERQELPNIR VANGDAIILL REMLAPGSLA GLRVYFPDPW PKKRHHKRRL 

       190        200        210        220        230        240 
IQPEFLTLAA TRLAPGAVVH CATDWEPYAE QMLDVLTAHP DFENTVPGGG FAPRPEHRPL 

       250        260        270 
TRFEGQGLDK GHVVNDLLFR RVQHKEPPPN G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL939118 Genomic DNA. Translation: CAC08407.1.
RefSeqNP_628290.1. NC_003888.3.

3D structure databases

ProteinModelPortalQ9F305.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO4111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC08407; CAC08407; CAC08407.
GeneID1099548.
KEGGsco:SCO4111.
PATRIC23738006. VBIStrCoe124346_4171.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
PhylomeDBQ9F305.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STRCO
AccessionPrimary (citable) accession number: Q9F305
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways