Q9F284 (CGIA_ZOBGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Iota-carrageenase
EC=3.2.1.157

Gene names

Name:	cgiA
Ordered Locus Names:	zobellia_4265

Organism

Zobellia galactanivorans (strain DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij) [Complete proteome] [HAMAP]

Taxonomic identifier

63186 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Zobellia

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes iota-carrageenans, sulfated 1,3-alpha-1,4-beta galactans from red algal cell walls, with an inversion of anomeric configuration. Also active against hybrid iota-/nu-carrageenan, not active against kappa- or lambda-carrageenans. Ref.1 UniProtKB Q9F5I8
Catalytic activity	Endohydrolysis of 1,4-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans. Ref.1
Subcellular location	Secreted › extracellular space Ref.1.
Sequence similarities	Belongs to the glycosyl hydrolase 82 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iota-carrageenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 491	472	Iota-carrageenase		PRO_5000065975
Amino acid modifications
	Disulfide bond	422 ↔ 490		By similarity UniProtKB Q9F5I8

Sequences

	Sequence		Length	Mass (Da)
	Q9F284 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: AE310457609FC77C	FASTA	491	53,395
	10 20 30 40 50 60 MKLQFKPVYL ASIAIMAIGC TKEVTENDTS EISEVPTELR AAASSFYTPP GQNVRANKKN 70 80 90 100 110 120 LVTDYGVNHN DQNDDSSKLN LAIKDLSDTG GILTLPKGKY YLTKIRMRSN VHLEIEKGTV 130 140 150 160 170 180 IYPTKGLTPA KNHRIFDFAS KTEEKIENAS IVGKGGKFIV DLRGNSSKNQ IVADVGNVTN 190 200 210 220 230 240 FKISNFTIKD EKTIFASILV SFTDKAGNAW PHKGIIENID QANAHTGYGL IQAYAADNIL 250 260 270 280 290 300 FNNLSCTGGV TLRLETDNLA MKTAKKGGVR DIFATKIKNT NGLTPVMFSP HFMENGKVTI 310 320 330 340 350 360 DDVTAIGCAY AVRVEHGFIE IFDKGNRASA DAFKNYIEGI LGAGSVEVVY KRNNGRTWAA 370 380 390 400 410 420 RIANDFNEAA YNHSNPAVSG IKPGKFATSK VTNVKATYKG TGAKLKQAFL SYLPCSERSK 430 440 450 460 470 480 VCRPGPDGFE YNGPSLGVTI DNTKRDNSLG NYNVNVSTSS VQGFPNNYVL NVKYNTPKVC 490 NQNLGSITSC N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"iota-Carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of kappa-carrageenases." Barbeyron T., Michel G., Potin P., Henrissat B., Kloareg B. J. Biol. Chem. 275:35499-35505(2000) [PubMed: 10934194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-48 AND 395-399, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION. Strain: DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij.
[2]	"Complete genome sequence of Zobellia galactanivorans Dsij." Genoscope - CEA Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 12802 / CIP 106680 / NCIMB 13871 / Dsij.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ272071 Genomic DNA. Translation: CAC07822.1. FP476056 Genomic DNA. Translation: CAZ98400.1.
RefSeq	YP_004738679.1. NC_015844.1.
3D structure databases
HSSP	HSSP built from PDB template 1H80 based on UniProtKB Q9F5I8.
ProteinModelPortal	Q9F284.
ModBase	Search...
Protein family/group databases
CAZy	GH82. Glycoside Hydrolase Family 82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10978568.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16653.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CGIA_ZOBGA

Accession

Primary (citable) accession number: Q9F284
Secondary accession number(s): G0KZI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program