Reviewed, UniProtKB/Swiss-Prot Q9F234 (AGL2_BACTQ)

Names and origin

Protein names	Recommended name:     Alpha-glucosidase 2     EC=3.2.1.20 Alternative name(s):     Alpha-glucosidase II
Organism	Bacillus thermoamyloliquefaciens
Taxonomic identifier	1425 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	787 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 787	787	Alpha-glucosidase 2		PRO_0000185368
Sites
	Active site	407	1	By similarity
	Active site	410	1	By similarity
	Active site	484	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9F234-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6BDD837E2C3304BF	FASTA	787	91,072
	10 20 30 40 50 60 MLEDTSFAIQ PEQDDKTQET HRIDIGNMHT FSHTEHVFSF HCDTGIVKIR FYREDIVRIA 70 80 90 100 110 120 FNPFGETSLS TSVAVVKEPE KVDASVHETE EEVTLTSAKQ TVVLQKRPFR VRIYDNHGRL 130 140 150 160 170 180 LVAEGKKGMA FTYQGEVCCF KMMDEADHFY GFGEKTGFLD KRGETMTMWN TDVYAPHNPE 190 200 210 220 230 240 TDPLYQSHPY FMTVRNGSAH GIFFDNTYKT TFDFQTATDE YCFSAEGGAI DYYVFAGPTP 250 260 270 280 290 300 KDVLEQYTDL TGRMPLPPKW ALGYHQSRYS YETEQEVREI AQTFIEKDIP LDVIYLDIHY 310 320 330 340 350 360 MNGYRVFTFD RNRFPNLKQL IADLKQKGIR VVPIVDPGVK EDPEYVIYQE GIRHDYFCKY 370 380 390 400 410 420 IEGNVYFGEV WPGKSAFPDF TNKKVRKWWG EKHQFYTDLG IEGIWNDMNE PSVFNETKTM 430 440 450 460 470 480 DVKVIHDNDG DPKTHRELHN VYGFMMGEAT YKGMKKLLNG KRPFLLTRAG FSGIQRYAAV 490 500 510 520 530 540 WTGDNRSFWE HLQMSLPMCM NLGLSGVAFC GPDVGGFAHN TNGELLTRWM QVGAFTPYFR 550 560 570 580 590 600 NHCAIGFRRQ EPWAFGEKYE RIIKKYIRLR YQWLPHLYTL FAEAHETGAP VMRPLFFEYP 610 620 630 640 650 660 DDENTYNLYD EFLVGANVLI APIMTPSTTR RVAYFPKGNW VDYWTGEVLE GGQYHLISAD 670 680 690 700 710 720 LETLPIFIKQ GSAIALGDVK RSTEMPDEHR TVHIYKANGG KATYVLYDDD GQTFSYEKGD 730 740 750 760 770 780 YLRMYIEVEY GENSVHIVTK SEGTYQPSWK LSFAIHHATE QTKVTIDGNE QNAIFDPHQR ILLIQSE

References

[1]

"The primary structure of the subunit in Bacillus thermoamyloliquefaciens KP1071 molecular weight 540,000 homohexameric alpha-glucosidase II belonging to the glycosyl hydrolase family 31." Kashiwabara S., Azuma S., Tsuduki M., Suzuki Y. Biosci. Biotechnol. Biochem. 64:1379-1393(2000) [PubMed: 10945254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KP1071.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB012238 Genomic DNA. Translation: BAA76396.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Enzyme and pathway databases
BRENDA	3.2.1.20. 288695.
Family and domain databases
InterPro	IPR000322. Glyco_hydro_31. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGL2_BACTQ

Accession

Primary (citable) accession number: Q9F234

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	April 14, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)