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Protein

Holliday junction ATP-dependent DNA helicase RuvA

Gene

ruvA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvAUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvAUniRule annotation
Ordered Locus Names:TTHA0291
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947011 – 191Holliday junction ATP-dependent DNA helicase RuvAAdd BLAST191

Interactioni

Subunit structurei

Forms a complex with RuvB.

Protein-protein interaction databases

IntActiQ9F1Q3. 1 interactor.
MINTiMINT-88028.
STRINGi300852.TTHA0291.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 12Combined sources9
Beta strandi14 – 20Combined sources7
Beta strandi22 – 29Combined sources8
Helixi32 – 37Combined sources6
Beta strandi42 – 48Combined sources7
Beta strandi59 – 64Combined sources6
Helixi65 – 75Combined sources11
Beta strandi76 – 78Combined sources3
Helixi82 – 91Combined sources10
Helixi94 – 102Combined sources9
Helixi106 – 109Combined sources4
Helixi117 – 127Combined sources11
Turni128 – 130Combined sources3
Helixi144 – 155Combined sources12
Helixi160 – 173Combined sources14
Helixi179 – 187Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IXRX-ray3.30A/B1-191[»]
1IXSX-ray3.20A130-191[»]
ProteinModelPortaliQ9F1Q3.
SMRiQ9F1Q3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9F1Q3.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057115.
KOiK03550.
OMAiRNQETIF.
PhylomeDBiQ9F1Q3.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9F1Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRYLRGLVL KKEAGGFVLL AGGVGFFLQA PTPFLQALEE GKEVGVHTHL
60 70 80 90 100
LLKEEGLSLY GFPDEENLAL FELLLSVSGV GPKVALALLS ALPPRLLARA
110 120 130 140 150
LLEGDARLLT SASGVGRRLA ERIALELKGK VPPHLLAGEK VESEAAEEAV
160 170 180 190
MALAALGFKE AQARAVVLDL LAQNPKARAQ DLIKEALKRL R
Length:191
Mass (Da):20,414
Last modified:March 1, 2001 - v1
Checksum:i1DA0CADFDD5EBC3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048605 Genomic DNA. Translation: BAB18786.1.
AP008226 Genomic DNA. Translation: BAD70114.1.
RefSeqiWP_011174059.1. NC_006461.1.
YP_143557.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70114; BAD70114; BAD70114.
GeneIDi3168157.
KEGGittj:TTHA0291.
PATRICi23955537. VBITheThe93045_0291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB048605 Genomic DNA. Translation: BAB18786.1.
AP008226 Genomic DNA. Translation: BAD70114.1.
RefSeqiWP_011174059.1. NC_006461.1.
YP_143557.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IXRX-ray3.30A/B1-191[»]
1IXSX-ray3.20A130-191[»]
ProteinModelPortaliQ9F1Q3.
SMRiQ9F1Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9F1Q3. 1 interactor.
MINTiMINT-88028.
STRINGi300852.TTHA0291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70114; BAD70114; BAD70114.
GeneIDi3168157.
KEGGittj:TTHA0291.
PATRICi23955537. VBITheThe93045_0291.

Phylogenomic databases

eggNOGiENOG4105KA4. Bacteria.
COG0632. LUCA.
HOGENOMiHOG000057115.
KOiK03550.
OMAiRNQETIF.
PhylomeDBiQ9F1Q3.

Miscellaneous databases

EvolutionaryTraceiQ9F1Q3.

Family and domain databases

CDDicd14332. UBA_RuvA_C. 1 hit.
HAMAPiMF_00031. DNA_helic_RuvA. 1 hit.
InterProiIPR013849. DNA_helicase_Holl-junc_RuvA_I.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR000085. RuvA.
IPR010994. RuvA_2-like.
IPR011114. RuvA_C.
[Graphical view]
PfamiPF07499. RuvA_C. 1 hit.
PF01330. RuvA_N. 1 hit.
[Graphical view]
SMARTiSM00278. HhH1. 2 hits.
[Graphical view]
SUPFAMiSSF46929. SSF46929. 1 hit.
SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00084. ruvA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVA_THET8
AccessioniPrimary (citable) accession number: Q9F1Q3
Secondary accession number(s): Q5SLK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.