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Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Ordered Locus Names:SAOUHSC_01811
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10651065DNA polymerase III subunit alphaPRO_0000103342Add
BLAST

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01811.

Structurei

3D structure databases

ProteinModelPortaliQ9F1K0.
SMRiQ9F1K0. Positions 3-887.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C0B. Bacteria.
COG0587. LUCA.
HOGENOMiHOG000021785.
KOiK02337.
OMAiQIMLNSA.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9F1K0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAYLNIHTA YDLLNSSLKI EDAVRLAVSE NVDALAITDT NVLYGFPKFY
60 70 80 90 100
DACIANNIKP IFGMTIYVTN GLNTVETVVL AKNNDGLKDL YQLSSEIKMN
110 120 130 140 150
ALEHVSFELL KRFSNNMIII FKKVGDQHRD IVQVFETHND TYMDHLSISI
160 170 180 190 200
QGRKHVWIQN VCYQTRQDAD TISALAAIRD NTKLDLIHDQ EDFGAHFLTE
210 220 230 240 250
KEINQLDINQ EYLTQVDVIA QKCDAELKYH QSLLPQYETP NDESAKKYLW
260 270 280 290 300
RVLVTQLKKL ELNYDVYLER LKYEYKVITN MGFEDYFLIV SDLIHYAKTN
310 320 330 340 350
DVMVGPGRGS SAGSLVSYLL GITTIDPIKF NLLFERFLNP ERVTMPDIDI
360 370 380 390 400
DFEDTRRERV IQYVQEKYGE LHVSGIVTFG HLLARAVARD VGRIMGFDEV
410 420 430 440 450
TLNEISSLIP HKLGITLDEA YQIDDFKEFV HRNHRHERWF SICKKLEGLP
460 470 480 490 500
RHTSTHAAGI IINDHPLYEY APLTKGDTGL LTQWTMTEAE RIGLLKIDFL
510 520 530 540 550
GLRNLSIIHQ ILTQVKKDLG INIDIEKIPF DDQKVFELLS QGDTTGIFQL
560 570 580 590 600
ESDGVRSVLK KLKPEHFEDI VAVTSLYRPG PMEEIPTYIT RRHDPSKVQY
610 620 630 640 650
LHPHLEPILK NTYGVIIYQE QIMQIASTFA NFSYGEADIL RRAMSKKNRA
660 670 680 690 700
VLESERQHFI EGAKQNGYHE DISKQIFDLI LKFADYGFPR AHAVSYSKIA
710 720 730 740 750
YIMSFLKVHY PNYFYANILS NVIGSEKKTA QMIEEAKKQG ITILPPNINE
760 770 780 790 800
SHWFYKPSQE GIYLSIGTIK GVGYQSVKVI VDERYQNGKF KDFFDFARRI
810 820 830 840 850
PKRVKTRKLL EALILVGAFD AFGKTRSTLL QAIDQVLDGD LNIEQDGFLF
860 870 880 890 900
DILTPKQMYE DKEELPDALI SQYEKEYLGF YVSQHPVDKK FVAKQYLTIF
910 920 930 940 950
KLSNAQNYKP ILVQFDKVKQ IRTKNGQNMA FVTLNDGIET LDGVIFPNQF
960 970 980 990 1000
KKYEELLSHN DLFIVSGKFD HRKQQRQLII NEIQTLATFE EQKLAFAKQI
1010 1020 1030 1040 1050
IIRNKSQIDM FEEMIKATKE NANDVVLSFY DETIKQMTTL GYINQKDSMF
1060
NNFIQSFNPS DIRLI
Length:1,065
Mass (Da):122,965
Last modified:March 1, 2001 - v1
Checksum:iB7E4196B91EBCF7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053352 Genomic DNA. Translation: BAB20884.1.
CP000253 Genomic DNA. Translation: ABD30879.1.
RefSeqiYP_500316.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD30879; ABD30879; SAOUHSC_01811.
GeneIDi3919281.
KEGGisao:SAOUHSC_01811.
PATRICi19580981. VBIStaAur99865_1651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB053352 Genomic DNA. Translation: BAB20884.1.
CP000253 Genomic DNA. Translation: ABD30879.1.
RefSeqiYP_500316.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ9F1K0.
SMRiQ9F1K0. Positions 3-887.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_01811.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD30879; ABD30879; SAOUHSC_01811.
GeneIDi3919281.
KEGGisao:SAOUHSC_01811.
PATRICi19580981. VBIStaAur99865_1651.

Phylogenomic databases

eggNOGiENOG4105C0B. Bacteria.
COG0587. LUCA.
HOGENOMiHOG000021785.
KOiK02337.
OMAiQIMLNSA.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-1730-MONOMER.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3A_STAA8
AccessioniPrimary (citable) accession number: Q9F1K0
Secondary accession number(s): Q2FXM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.