Skip Header

Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q9F0W4 (NOSZ_PSEFL)

Last modified February 9, 2010. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase
Gene names
Name: nosZ
OrganismPseudomonas fluorescens
Taxonomic identifier294 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Hide | Top

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Function

Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity. HAMAP MF_00716

Catalytic activity

N2 + H2O + acceptor = N2O + reduced acceptor. HAMAP MF_00716

Cofactor

Binds 2 calcium ions per subunit By similarity. HAMAP MF_00716

Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity. HAMAP MF_00716

Pathway

Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4. HAMAP MF_00716

Subunit structure

Homodimer By similarity. HAMAP MF_00716

Subcellular location

Periplasm By similarity HAMAP MF_00716.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_00716

Sequence similarities

Belongs to the nosZ family.

In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5454Tat-type signal Potential
Chain55 – 639585Nitrous-oxide reductase HAMAP MF_00716
PRO_0000019830

Regions

Region543 – 63997COX2-like HAMAP MF_00716

Sites

Metal binding1301Copper Z2 By similarity
Metal binding1311Copper Z3 By similarity
Metal binding1791Copper Z2 By similarity
Metal binding2571Calcium 2; via carbonyl oxygen By similarity
Metal binding2601Calcium 2 By similarity
Metal binding2681Calcium 2; via carbonyl oxygen By similarity
Metal binding2741Calcium 2 By similarity
Metal binding3251Calcium 2 By similarity
Metal binding3271Copper Z1 By similarity
Metal binding3831Copper Z1 By similarity
Metal binding4341Copper Z3 By similarity
Metal binding4551Calcium 1; via carbonyl oxygen By similarity
Metal binding4701Calcium 1 By similarity
Metal binding4951Copper Z4 By similarity
Metal binding5841Copper A1 By similarity
Metal binding6191Copper A1 By similarity
Metal binding6191Copper A2 By similarity
Metal binding6211Copper A2; via carbonyl oxygen By similarity
Metal binding6231Copper A1 By similarity
Metal binding6231Copper A2 By similarity
Metal binding6271Copper A2 By similarity
Metal binding6301Copper A1 By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q9F0W4-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: D9B8809D115E58C8

FASTA63971,107
        10         20         30         40         50         60 
MSDKKDQVPG AVEAPRGVSR RSFLGTGAVT GAVLAGATAL GAGTFTRESW AAAAKEAKSK 

        70         80         90        100        110        120 
IHVGPGELDE YYGFWSGGHQ GEVRVLGVPS MRELMRIPVF NVDSATGWGL TNESKRILGD 

       130        140        150        160        170        180 
SAKYQNGDCH HPHISMTDGK YDGKYLFIND KANSRVARIR LDIMKCDKIL TVPNVQAIHG 

       190        200        210        220        230        240 
LRLQKVPYTK YVFANAEFVI PHPNDGHTFD LQDKNSFTMF NAIDAEKMEM AFQVIVDGNL 

       250        260        270        280        290        300 
DNSDADYTGK YAASTCYNSE KAYDLGGMMR NERDWVVVFN ILRIEAAIKA GKFITLDGSK 

       310        320        330        340        350        360 
VPVVDGRKTD GKDSEFTRYI PVPKNPHGLN TSSDGKYFIA NGKLSPTVSM IAIDRLDDLF 

       370        380        390        400        410        420 
NDRYKDPREV IVAEPELGLG PLHTTFDGRG NAYTTLFIDS QVVKWNMDEA IRAYKGEKVN 

       430        440        450        460        470        480 
YIKQKLDVQY QPGHNHASLT ETSEADGKWL VVLCKFSKDR FLPTGPLHPE NDQLIDISGE 

       490        500        510        520        530        540 
EMKLVHDGPA FAEPHDCILA RRDQIKTQKI WNRNDPFFAD TVALAKKDGI NLETDNKVIR 

       550        560        570        580        590        600 
DGNKVRVYMT SMAPAYGLTE FTVKQGNEVT VTITNIDQIE DVSHGFVMTN HGASMEISPQ 

       610        620        630 
QTSSITFTAD KAGLHWYYCS WFCHALHMEM VGRMLVEKA

« Hide

Hide | Top

References

[1]"Characterization and transcriptional analysis of Pseudomonas fluorescens denitrifying clusters containing the nar, nir, nor and nos genes."
Philippot L., Mirleau P., Mazurier S., Siblot S., Hartmann A., Lemanceau P., Germon J.C.
Biochim. Biophys. Acta 1517:436-440(2001) [PubMed: 11342223] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C7R12.
[2]Philippot L., Cheneby D., Hartmann A., Germon J.C.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-613.
Strain: C7R12.

Hide | Top

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF197468 Genomic DNA. Translation: AAG34386.1.
AF056319 Genomic DNA. Translation: AAD22389.1.

3D structure databases

SMRQ9F0W4. Positions 62-639.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.7.99.6. 329.

Family and domain databases

HAMAPMF_00716. NosZ.
[Tree]
InterProIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011045. N2O_reductase_N.
IPR006311. TAT_signal.
IPR017909. Twin_arg_translocation_Tat.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
Gene3DG3DSA:2.60.40.420. Cupredoxin. 1 hit.
G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
PfamPF00116. COX2. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameNOSZ_PSEFL
AccessionPrimary (citable) accession number: Q9F0W4
Secondary accession number(s): Q9X2U9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: March 1, 2001
Last modified: February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents