Reviewed, UniProtKB/Swiss-Prot Q9F0W4 (NOSZ_PSEFL)

Names and origin

Protein names

Recommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase

Gene names

Name:

nosZ

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity.
Catalytic activity	N2 + H2O + acceptor = N2O + reduced acceptor. HAMAP MF_00716
Cofactor	Binds 2 calcium ions per subunit By similarity. Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4. HAMAP MF_00716
Subunit structure	Homodimer By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_00716
Sequence similarities	Belongs to the nosZ family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Copper Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: HAMAP copper ion binding Inferred from electronic annotation. Source: HAMAP cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro nitrous-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 54	54	Tat-type signal Potential
	Chain	55 – 639	585	Nitrous-oxide reductase HAMAP MF_00716		PRO_0000019830
Regions
	Region	543 – 639	97	COX2-like HAMAP MF_00716
Sites
	Metal binding	130	1	Copper Z2 By similarity
	Metal binding	131	1	Copper Z3 By similarity
	Metal binding	179	1	Copper Z2 By similarity
	Metal binding	257	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	260	1	Calcium 2 By similarity
	Metal binding	268	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	274	1	Calcium 2 By similarity
	Metal binding	325	1	Calcium 2 By similarity
	Metal binding	327	1	Copper Z1 By similarity
	Metal binding	383	1	Copper Z1 By similarity
	Metal binding	434	1	Copper Z3 By similarity
	Metal binding	455	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	470	1	Calcium 1 By similarity
	Metal binding	495	1	Copper Z4 By similarity
	Metal binding	584	1	Copper A1 By similarity
	Metal binding	619	1	Copper A1 By similarity
	Metal binding	619	1	Copper A2 By similarity
	Metal binding	621	1	Copper A2; via carbonyl oxygen By similarity
	Metal binding	623	1	Copper A1 By similarity
	Metal binding	623	1	Copper A2 By similarity
	Metal binding	627	1	Copper A2 By similarity
	Metal binding	630	1	Copper A1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9F0W4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D9B8809D115E58C8	FASTA	639	71,107
	10 20 30 40 50 60 MSDKKDQVPG AVEAPRGVSR RSFLGTGAVT GAVLAGATAL GAGTFTRESW AAAAKEAKSK 70 80 90 100 110 120 IHVGPGELDE YYGFWSGGHQ GEVRVLGVPS MRELMRIPVF NVDSATGWGL TNESKRILGD 130 140 150 160 170 180 SAKYQNGDCH HPHISMTDGK YDGKYLFIND KANSRVARIR LDIMKCDKIL TVPNVQAIHG 190 200 210 220 230 240 LRLQKVPYTK YVFANAEFVI PHPNDGHTFD LQDKNSFTMF NAIDAEKMEM AFQVIVDGNL 250 260 270 280 290 300 DNSDADYTGK YAASTCYNSE KAYDLGGMMR NERDWVVVFN ILRIEAAIKA GKFITLDGSK 310 320 330 340 350 360 VPVVDGRKTD GKDSEFTRYI PVPKNPHGLN TSSDGKYFIA NGKLSPTVSM IAIDRLDDLF 370 380 390 400 410 420 NDRYKDPREV IVAEPELGLG PLHTTFDGRG NAYTTLFIDS QVVKWNMDEA IRAYKGEKVN 430 440 450 460 470 480 YIKQKLDVQY QPGHNHASLT ETSEADGKWL VVLCKFSKDR FLPTGPLHPE NDQLIDISGE 490 500 510 520 530 540 EMKLVHDGPA FAEPHDCILA RRDQIKTQKI WNRNDPFFAD TVALAKKDGI NLETDNKVIR 550 560 570 580 590 600 DGNKVRVYMT SMAPAYGLTE FTVKQGNEVT VTITNIDQIE DVSHGFVMTN HGASMEISPQ 610 620 630 QTSSITFTAD KAGLHWYYCS WFCHALHMEM VGRMLVEKA

References

[1]	"Characterization and transcriptional analysis of Pseudomonas fluorescens denitrifying clusters containing the nar, nir, nor and nos genes." Philippot L., Mirleau P., Mazurier S., Siblot S., Hartmann A., Lemanceau P., Germon J.C. Biochim. Biophys. Acta 1517:436-440(2001) [PubMed: 11342223] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C7R12.
[2]	Philippot L., Cheneby D., Hartmann A., Germon J.C. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-613. Strain: C7R12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF197468 Genomic DNA. Translation: AAG34386.1. AF056319 Genomic DNA. Translation: AAD22389.1.
3D structure databases
HSSP	HSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.7.99.6. 329.
Family and domain databases
HAMAP	MF_00716. [Tree]
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. IPR015943. WD40/YVTN_repeat-like. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00116. COX2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOSZ_PSEFL

Accession

Primary (citable) accession number: Q9F0W4
Secondary accession number(s): Q9X2U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)