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Q9EZQ7

- BLAC_NOCAS

UniProt

Q9EZQ7 - BLAC_NOCAS

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Protein

Beta-lactamase AST-1

Gene
bla, ast1
Organism
Nocardia asteroides
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulationi

Inhibited by clavulanic acid.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin
  3. KM=7 µM for ticarcillin
  4. KM=330 µM for piperacillin
  5. KM=20 µM for cephalothin

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase AST-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:ast1
OrganismiNocardia asteroides
Taxonomic identifieri1824 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed predictionAdd
BLAST
Chaini32 – 310279Beta-lactamase AST-1PRO_0000313797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi32 – 321N-palmitoyl cysteine Reviewed prediction
Lipidationi32 – 321S-diacylglycerol cysteine Reviewed prediction

Keywords - PTMi

Lipoprotein, Palmitate

Structurei

3D structure databases

ProteinModelPortaliQ9EZQ7.
SMRiQ9EZQ7. Positions 52-310.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni255 – 2573Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EZQ7-1 [UniParc]FASTAAdd to Basket

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MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV    50
ADPRFAELET TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA 100
LLREHPLSTG YFDQVIHYSA AELVEYSPVT ETRVETGMTV RELCDAAITV 150
SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT SRLDRWETDL NTAIPGDERD 200
TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT RIRAGLPADW 250
TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE 300
ATRVVVDALG 310
Length:310
Mass (Da):32,476
Last modified:March 1, 2001 - v1
Checksum:i53668F7DBFF1A6B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF279904 Genomic DNA. Translation: AAG44836.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF279904 Genomic DNA. Translation: AAG44836.1 .

3D structure databases

ProteinModelPortali Q9EZQ7.
SMRi Q9EZQ7. Positions 52-310.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto."
    Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P.
    Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiBLAC_NOCAS
AccessioniPrimary (citable) accession number: Q9EZQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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