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Q9EZQ7 (BLAC_NOCAS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase AST-1

EC=3.5.2.6
Gene names
Name:bla
Synonyms:ast1
OrganismNocardia asteroides
Taxonomic identifier1824 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Inhibited by clavulanic acid. Ref.1

Subcellular location

Cell membrane; Lipid-anchor By similarity.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Biophysicochemical properties

Kinetic parameters:

KM=30 µM for benzylpenicillin Ref.1

KM=50 µM for amoxicillin

KM=7 µM for ticarcillin

KM=330 µM for piperacillin

KM=20 µM for cephalothin

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionHydrolase
   PTMLipoprotein
Palmitate
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 310279Beta-lactamase AST-1
PRO_0000313797

Regions

Region255 – 2573Substrate binding By similarity

Sites

Active site911Acyl-ester intermediate By similarity

Amino acid modifications

Lipidation321N-palmitoyl cysteine Potential
Lipidation321S-diacylglycerol cysteine Potential

Sequences

Sequence LengthMass (Da)Tools
Q9EZQ7 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 53668F7DBFF1A6B3

FASTA31032,476
        10         20         30         40         50         60 
MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV ADPRFAELET 

        70         80         90        100        110        120 
TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA LLREHPLSTG YFDQVIHYSA 

       130        140        150        160        170        180 
AELVEYSPVT ETRVETGMTV RELCDAAITV SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT 

       190        200        210        220        230        240 
SRLDRWETDL NTAIPGDERD TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT 

       250        260        270        280        290        300 
RIRAGLPADW TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE 

       310 
ATRVVVDALG 

« Hide

References

[1]"Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto."
Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P.
Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF279904 Genomic DNA. Translation: AAG44836.1.

3D structure databases

ProteinModelPortalQ9EZQ7.
SMRQ9EZQ7. Positions 52-310.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAC_NOCAS
AccessionPrimary (citable) accession number: Q9EZQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families