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Q9EZQ7

- BLAC_NOCAS

UniProt

Q9EZQ7 - BLAC_NOCAS

Protein

Beta-lactamase AST-1

Gene

bla

Organism
Nocardia asteroides
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited by clavulanic acid.1 Publication

    Kineticsi

    1. KM=30 µM for benzylpenicillin1 Publication
    2. KM=50 µM for amoxicillin1 Publication
    3. KM=7 µM for ticarcillin1 Publication
    4. KM=330 µM for piperacillin1 Publication
    5. KM=20 µM for cephalothin1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase AST-1 (EC:3.5.2.6)
    Gene namesi
    Name:bla
    Synonyms:ast1
    OrganismiNocardia asteroides
    Taxonomic identifieri1824 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 310279Beta-lactamase AST-1PRO_0000313797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi32 – 321N-palmitoyl cysteineSequence Analysis
    Lipidationi32 – 321S-diacylglycerol cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Palmitate

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EZQ7.
    SMRiQ9EZQ7. Positions 52-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni255 – 2573Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9EZQ7-1 [UniParc]FASTAAdd to Basket

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    MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV    50
    ADPRFAELET TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA 100
    LLREHPLSTG YFDQVIHYSA AELVEYSPVT ETRVETGMTV RELCDAAITV 150
    SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT SRLDRWETDL NTAIPGDERD 200
    TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT RIRAGLPADW 250
    TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE 300
    ATRVVVDALG 310
    Length:310
    Mass (Da):32,476
    Last modified:March 1, 2001 - v1
    Checksum:i53668F7DBFF1A6B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF279904 Genomic DNA. Translation: AAG44836.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF279904 Genomic DNA. Translation: AAG44836.1 .

    3D structure databases

    ProteinModelPortali Q9EZQ7.
    SMRi Q9EZQ7. Positions 52-310.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto."
      Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P.
      Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBLAC_NOCAS
    AccessioniPrimary (citable) accession number: Q9EZQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3