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Protein

Beta-lactamase AST-1

Gene

bla

Organism
Nocardia asteroides
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin1 Publication
  3. KM=7 µM for ticarcillin1 Publication
  4. KM=330 µM for piperacillin1 Publication
  5. KM=20 µM for cephalothin1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei91 – 911Acyl-ester intermediateBy similarity
Sitei94 – 941Increases nucleophilicity of active site SerBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Active sitei187 – 1871Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase AST-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:ast1
OrganismiNocardia asteroides
Taxonomic identifieri1824 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Subcellular locationi

Cell membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 310279Beta-lactamase AST-1PRO_0000313797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi32 – 321N-palmitoyl cysteineSequence Analysis
Lipidationi32 – 321S-diacylglycerol cysteineSequence Analysis

Keywords - PTMi

Lipoprotein, Palmitate

Structurei

3D structure databases

ProteinModelPortaliQ9EZQ7.
SMRiQ9EZQ7. Positions 52-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni255 – 2573Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EZQ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV
60 70 80 90 100
ADPRFAELET TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA
110 120 130 140 150
LLREHPLSTG YFDQVIHYSA AELVEYSPVT ETRVETGMTV RELCDAAITV
160 170 180 190 200
SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT SRLDRWETDL NTAIPGDERD
210 220 230 240 250
TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT RIRAGLPADW
260 270 280 290 300
TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE
310
ATRVVVDALG
Length:310
Mass (Da):32,476
Last modified:March 1, 2001 - v1
Checksum:i53668F7DBFF1A6B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF279904 Genomic DNA. Translation: AAG44836.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF279904 Genomic DNA. Translation: AAG44836.1.

3D structure databases

ProteinModelPortaliQ9EZQ7.
SMRiQ9EZQ7. Positions 52-310.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto."
    Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P.
    Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiBLAC_NOCAS
AccessioniPrimary (citable) accession number: Q9EZQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.