Q9EZQ7 (BLAC_NOCAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase AST-1
EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	ast1

Organism

Nocardia asteroides

Taxonomic identifier

1824 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Confers high levels of resitance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by clavulanic acid. Ref.1
Subcellular location	Cell membrane; Lipid-anchor By similarity.
Sequence similarities	Belongs to the class-A beta-lactamase family.
Biophysicochemical properties	Kinetic parameters: KM=30 µM for benzylpenicillin Ref.1 KM=50 µM for amoxicillin KM=7 µM for ticarcillin KM=330 µM for piperacillin KM=20 µM for cephalothin

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cell membrane Membrane
Domain	Signal
Molecular function	Hydrolase
PTM	Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 310	279	Beta-lactamase AST-1		PRO_0000313797
Regions
	Region	255 – 257	3	Substrate binding By similarity
Sites
	Active site	91	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Lipidation	32	1	N-palmitoyl cysteine Potential
	Lipidation	32	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9EZQ7 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 53668F7DBFF1A6B3	FASTA	310	32,476
	10 20 30 40 50 60 MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV ADPRFAELET 70 80 90 100 110 120 TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA LLREHPLSTG YFDQVIHYSA 130 140 150 160 170 180 AELVEYSPVT ETRVETGMTV RELCDAAITV SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT 190 200 210 220 230 240 SRLDRWETDL NTAIPGDERD TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT 250 260 270 280 290 300 RIRAGLPADW TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE 310 ATRVVVDALG

References

[1]	"Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto." Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P. Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed: 11181374] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF279904 Genomic DNA. Translation: AAG44836.1.
3D structure databases
HSSP	HSSP built from PDB template 1BSG based on UniProtKB P14559.
ProteinModelPortal	Q9EZQ7.
SMR	Q9EZQ7. Positions 52-310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51257. PROKAR_LIPOPROTEIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_NOCAS

Accession

Primary (citable) accession number: Q9EZQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2001
Last modified:	May 31, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program