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Protein

Beta-lactamase AST-1

Gene

bla

Organism
Nocardia asteroides
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Not active against ceftazidime, cefotaxime and aztreonam.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin1 Publication
  3. KM=7 µM for ticarcillin1 Publication
  4. KM=330 µM for piperacillin1 Publication
  5. KM=20 µM for cephalothin1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei91 – 911Acyl-ester intermediateBy similarity
    Sitei94 – 941Increases nucleophilicity of active site SerBy similarity
    Binding sitei151 – 1511SubstrateBy similarity
    Active sitei187 – 1871Proton acceptorBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase AST-1 (EC:3.5.2.6)
    Gene namesi
    Name:bla
    Synonyms:ast1
    OrganismiNocardia asteroides
    Taxonomic identifieri1824 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 310279Beta-lactamase AST-1PRO_0000313797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi32 – 321N-palmitoyl cysteineSequence Analysis
    Lipidationi32 – 321S-diacylglycerol cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Palmitate

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EZQ7.
    SMRiQ9EZQ7. Positions 52-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni255 – 2573Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9EZQ7-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTFSALPFRR ADRRRLLAAA LAACALTLTA ACDSGTVTVP VTDSVTTSAV
    60 70 80 90 100
    ADPRFAELET TSGARLGVFA VDTGSGRTVA HRADERFPMA STFKGLACGA
    110 120 130 140 150
    LLREHPLSTG YFDQVIHYSA AELVEYSPVT ETRVETGMTV RELCDAAITV
    160 170 180 190 200
    SDNTAGNQLL KLLGGPEGFT ASLRSLGDAT SRLDRWETDL NTAIPGDERD
    210 220 230 240 250
    TTTPAALAAD YRALVVGDVL GAPERDQLKA WLVANTTGAT RIRAGLPADW
    260 270 280 290 300
    TVGDKTGSPA YGSALDVAVA WPPGRAPIVI AVLSTKSEQD AEPDNALLAE
    310
    ATRVVVDALG
    Length:310
    Mass (Da):32,476
    Last modified:March 1, 2001 - v1
    Checksum:i53668F7DBFF1A6B3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF279904 Genomic DNA. Translation: AAG44836.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF279904 Genomic DNA. Translation: AAG44836.1.

    3D structure databases

    ProteinModelPortaliQ9EZQ7.
    SMRiQ9EZQ7. Positions 52-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. "Molecular and biochemical analysis of AST-1, a class A beta-lactamase from Nocardia asteroides sensu stricto."
      Poirel L., Laurent F., Naas T., Labia R., Boiron P., Nordmann P.
      Antimicrob. Agents Chemother. 45:878-882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiBLAC_NOCAS
    AccessioniPrimary (citable) accession number: Q9EZQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: March 1, 2001
    Last modified: January 7, 2015
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.