Q9EZ12 (DAPA_STAA8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	SAOUHSC_01396

Organism

Staphylococcus aureus (strain NCTC 8325)

Taxonomic identifier

93061 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103160
Regions
	Region	50 – 51	2	Pyruvate binding By similarity
Sites
	Active site	163	1	Schiff-base intermediate with substrate By similarity
	Binding site	108	1	Pyruvate By similarity
	Site	135	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9EZ12 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6ED1E5887F0C2C01	FASTA	295	32,584
	10 20 30 40 50 60 MTHLFEGVGV ALTTPFTNNK VNIEALKTHV NFLLENNAQA IIVNGTTAES PTLTTDEKER 70 80 90 100 110 120 ILKTVIDLVD KRVPVIAGTG TNDTEKSIQA SIQAKALGAD AIMLITPYYN KTNQRGLVKH 130 140 150 160 170 180 FEAIADAVKL PVVLYNVPSR TNMTIEPETV EILSQHPYIV ALKDATNDFE YLEEVKKRID 190 200 210 220 230 240 TNSFALYSGN DDNVVEYYQR GGQGVISVIA NVIPKEFQAL YDAQQSGLDI QDQFKPIGTL 250 260 270 280 290 LSALSVDINP IPIKALTSYL GFGNYELRLP LVSLEDTDTK VLRETYDTFK AGENE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and analysis of Staphylococcus aureus components expressed by a model system of growth in serum." Wiltshire M.D., Foster S.J. Infect. Immun. 69:5198-5202(2001) [PubMed: 11447207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Staphylococcus aureus NCTC8325 genome." Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 8325.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF306669 Genomic DNA. Translation: AAG42246.1. CP000253 Genomic DNA. Translation: ABD30490.1.
RefSeq	YP_499923.1. NC_007795.1.
3D structure databases
ProteinModelPortal	Q9EZ12.
SMR	Q9EZ12. Positions 2-293.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9EZ12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000029132; EBSTAP00000028083; EBSTAG00000029130.
GeneID	3920686.
GenomeReviews	Gene locus SAOUHSC_01396 in contig CP000253_GR.
KEGG	sao:SAOUHSC_01396.
PATRIC	19580235. VBIStaAur99865_1277.
Phylogenomic databases
eggNOG	COG0329.
GeneTree	EBGT00050000024245.
HOGENOM	HBG358848.
OMA	CEMEDSN.
PhylomeDB	Q9EZ12.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	SAUR93061:SAOUHSC_01396-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_STAA8

Accession

Primary (citable) accession number: Q9EZ12
Secondary accession number(s): Q2FYN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program