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Q9EYY6 (SYW_ENTAE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
OrganismEnterobacter aerogenes (Aerobacter aerogenes)
Taxonomic identifier548 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacter

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136639

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif195 – 1995"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site1981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9EYY6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: E212D77E5C720964

FASTA33437,505
        10         20         30         40         50         60 
MTKPIVFSGA QPSGELTIGN YMGALRQWVN MQDDYHCIYC IVDQHAITVR QDPQQLRKAT 

        70         80         90        100        110        120 
LDTLALYLAC GIDPQKSTIF VQSHVPEHAQ LGWALNCYTY FGELSRMTQF KDKSARYAEN 

       130        140        150        160        170        180 
INAGLFDYPV LMAADILLYQ TNQVPVGEDQ KQHLELSRDI AQRFNAIYGD IFKVPEPFIP 

       190        200        210        220        230        240 
KSGARVMSLL EPTKKMSKSD DNRNNVIGLL EDPKSVVKKI KRAVTDSDEP PVVRYDLKEK 

       250        260        270        280        290        300 
AGVSNLLDIL SAVTGKTIPE LEQHFEGKMY GHLKGEVAEA VSGMLIDLQE RYHRFRNDEA 

       310        320        330 
FLNQVMKDGA EKASARASQT LKAVYEAIGF VAKP 

« Hide

References

[1]"Alternative pathways for siroheme synthesis in Klebsiella aerogenes."
Kolko M.M., Kapetanovich L.A., Lawrence J.G.
J. Bacteriol. 183:328-335(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: W70.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF308467 Genomic DNA. Translation: AAG42458.1.

3D structure databases

ProteinModelPortalQ9EYY6.
SMRQ9EYY6. Positions 5-331.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_ENTAE
AccessionPrimary (citable) accession number: Q9EYY6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2001
Last modified: February 19, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries