Reviewed, UniProtKB/Swiss-Prot Q9EYW9 (DCEB_LISMO)

Names and origin

Protein names

Recommended name:
    Glutamate decarboxylase beta
      Short name=GAD-beta
    EC=4.1.1.15

Gene names

Name:	gadB
Ordered Locus Names:	lmo2363

Organism

Listeria monocytogenes [Complete proteome] [HAMAP]

Taxonomic identifier

1639 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Listeriaceae › Listeria

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Glutamate decarboxylase beta		PRO_0000146990
Amino acid modifications
	Modified residue	275	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	92	1	A → P in strain: LO28.
	Natural variant	124	1	E → D in strain: LO28.
	Natural variant	261	1	F → L in strain: EGD5.
	Natural variant	375	1	C → R in strain: EGD5.
	Natural variant	380 – 381	2	DD → TT in strain: LO28.

Sequences

	Sequence		Length	Mass (Da)
	Q9EYW9-1 [UniParc]. Last modified February 21, 2002. Version 2. Checksum: F2E2778CFD1E2C36	FASTA	464	53,543
	10 20 30 40 50 60 MLYSKENKES YLEPVFGSSA EDRDIPKYTL GKEPLEPRIA YRLVKDELLD EGSARQNLAT 70 80 90 100 110 120 FCQTYMEDEA TKLMSETLEK NAIDKSEYPR TAELENRCVN IIADLWHAPK DQKFMGTSTI 130 140 150 160 170 180 GSSEACMLGG MAMKFAWRKR AEKLGLDIYA KKPNLVISSG YQVCWEKFCV YWDIDMRVVP 190 200 210 220 230 240 MDKEHMQLNT DQVLDYVDEY TIGVVGILGI TYTGRYDDIY ALNEKLEEYN SKTDYKVYIH 250 260 270 280 290 300 VDAASGGFFT PFVEPDIIWD FRLKNVISIN TSGHKYGLVY PGIGWVLWKD ESYLPEELIF 310 320 330 340 350 360 KVSYLGGEMP TMQINFSRSA SHIIGQYYNF LRYGFEGYRT IHQKTSDVAQ YLAHAVEQTG 370 380 390 400 410 420 YFDIFNDGSH LPIVCYKLKD DANVNWTLYD LADRLQMRGW QVPAYPLPKS LENIIIQRYV 430 440 450 460 CRADLGFNMA EEFIQDFQAS IQELNNAHIL FHDTQQSGVH GFTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid." Cotter P.D., Gahan C.G.M., Hill C. Mol. Microbiol. 40:465-475(2001) [PubMed: 11309128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EGD5 and LO28 / Serovar 1/2c.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed: 11679669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e / Serovar 1/2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF309077 Genomic DNA. Translation: AAG22562.1. AF329447 Genomic DNA. Translation: AAK17187.1. AL591983 Genomic DNA. Translation: CAD00441.1.
PIR	AC1370.
RefSeq	NP_465886.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	985123.
GenomeReviews	Gene locus lmo2363 in contig AL591824_GR.
KEGG	lmo:lmo2363.
Organism-specific databases
ListiList	LMO02363.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9EYW9.
OMA	AVDEDTI.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2363-MON.
BRENDA	4.1.1.15. 96770.
Family and domain databases
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11999:SF1. Glu_decarb_GAD. 1 hit. PTHR11999. Pyridoxal_deC. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCEB_LISMO

Accession

Primary (citable) accession number: Q9EYW9
Secondary accession number(s): Q8Y4S0, Q9AGQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)