Q9EYW9 (DCEB_LISMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate decarboxylase beta
Short name=GAD-beta
EC=4.1.1.15

Gene names

Name:	gadB
Ordered Locus Names:	lmo2363

Organism

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e) [Reference proteome] [HAMAP]

Taxonomic identifier

169963 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › Listeria monocytogenes

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid.
Catalytic activity	L-glutamate = 4-aminobutanoate + CO2.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the group II decarboxylase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutamate decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Glutamate decarboxylase beta		PRO_0000146990
Amino acid modifications
	Modified residue	275	1	N6-(pyridoxal phosphate)lysine By similarity
Natural variations
	Natural variant	92	1	A → P in strain: LO28.
	Natural variant	124	1	E → D in strain: LO28.
	Natural variant	261	1	F → L in strain: EGD5.
	Natural variant	375	1	C → R in strain: EGD5.
	Natural variant	380 – 381	2	DD → TT in strain: LO28.

Sequences

	Sequence		Length	Mass (Da)
	Q9EYW9 [UniParc]. Last modified February 21, 2002. Version 2. Checksum: F2E2778CFD1E2C36	FASTA	464	53,543
	10 20 30 40 50 60 MLYSKENKES YLEPVFGSSA EDRDIPKYTL GKEPLEPRIA YRLVKDELLD EGSARQNLAT 70 80 90 100 110 120 FCQTYMEDEA TKLMSETLEK NAIDKSEYPR TAELENRCVN IIADLWHAPK DQKFMGTSTI 130 140 150 160 170 180 GSSEACMLGG MAMKFAWRKR AEKLGLDIYA KKPNLVISSG YQVCWEKFCV YWDIDMRVVP 190 200 210 220 230 240 MDKEHMQLNT DQVLDYVDEY TIGVVGILGI TYTGRYDDIY ALNEKLEEYN SKTDYKVYIH 250 260 270 280 290 300 VDAASGGFFT PFVEPDIIWD FRLKNVISIN TSGHKYGLVY PGIGWVLWKD ESYLPEELIF 310 320 330 340 350 360 KVSYLGGEMP TMQINFSRSA SHIIGQYYNF LRYGFEGYRT IHQKTSDVAQ YLAHAVEQTG 370 380 390 400 410 420 YFDIFNDGSH LPIVCYKLKD DANVNWTLYD LADRLQMRGW QVPAYPLPKS LENIIIQRYV 430 440 450 460 CRADLGFNMA EEFIQDFQAS IQELNNAHIL FHDTQQSGVH GFTH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A glutamate decarboxylase system protects Listeria monocytogenes in gastric fluid." Cotter P.D., Gahan C.G.M., Hill C. Mol. Microbiol. 40:465-475(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EGD5 and LO28 / Serovar 1/2c.
[2]	"Comparative genomics of Listeria species." Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F., Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A., Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E., Dominguez-Bernal G., Duchaud E. , Durant L., Dussurget O., Entian K.-D., Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W., Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U., Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A., Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B., Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N., Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P. Science 294:849-852(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-679 / EGD-e.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF309077 Genomic DNA. Translation: AAG22562.1. AF329447 Genomic DNA. Translation: AAK17187.1. AL591983 Genomic DNA. Translation: CAD00441.1.
PIR	AC1370.
RefSeq	NP_465886.1. NC_003210.1.
3D structure databases
ProteinModelPortal	Q9EYW9.
ModBase	Search...
Protein-protein interaction databases
STRING	169963.lmo2363.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD00441; CAD00441; CAD00441.
GeneID	985123.
KEGG	lmo:lmo2363.
PATRIC	20313958. VBILisMon69206_2421.
Organism-specific databases
GenoList	LMO2363.
Phylogenomic databases
eggNOG	COG0076.
HOGENOM	HOG000070228.
KO	K01580.
OMA	PMDKEHM.
ProtClustDB	CLSK876864.
Enzyme and pathway databases
BioCyc	LMON169963:LMO2363-MONOMER.
Family and domain databases
Gene3D	3.40.640.10. 1 hit.
InterPro	IPR010107. Glutamate_decarboxylase. IPR002129. PyrdxlP-dep_de-COase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfam	PF00282. Pyridoxal_deC. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01788. Glu-decarb-GAD. 1 hit.
PROSITE	PS00392. DDC_GAD_HDC_YDC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCEB_LISMO

Accession

Primary (citable) accession number: Q9EYW9
Secondary accession number(s): Q8Y4S0, Q9AGQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	February 21, 2002
Last modified:	May 29, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program