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Q9EYP3

- LIPA_BURPS

UniProt

Q9EYP3 - LIPA_BURPS

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Protein
Lipoyl synthase
Gene
lipA, lip, BPSL0414
Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi109 – 1091Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBPSE272560:GJNI-421-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Synonyms:lip
Ordered Locus Names:BPSL0414
OrganismiBurkholderia pseudomallei (strain K96243)
Taxonomic identifieri272560 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
ProteomesiUP000000605: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329Lipoyl synthaseUniRule annotation
PRO_0000102300Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272560.BPSL0414.

Structurei

3D structure databases

ProteinModelPortaliQ9EYP3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiFERIATN.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9EYP3-1 [UniParc]FASTAAdd to Basket

« Hide

MTDLTATPAP AEPAASAYDP TAKQKAQAKT ARIPIKIVPI EKLKKPEWIR    50
VKAATGSSRF NEIKTILREH NLHTVCEEAS CPNIGECFGK GTATFMIMGD 100
KCTRRCPFCD VGHGRPDPLD ADEPKNLART IAALKLKYVV ITSVDRDDLR 150
DGGAGHFVEC IREVREQSPA TRIEILTPDF RGRLDRALAI LNAAPPDVMN 200
HNLETVPRLY KEARPGSDYA HSLKLLKDFK ALHPDVATKS GLMVGLGETT 250
DEILQVMRDL RAHDVDMLTI GQYLQPSEHH LPVREYVHPD TFKMYEEEAY 300
KMGFTHAAVG AMVRSSYHAD LQAHGAGVV 329
Length:329
Mass (Da):36,429
Last modified:November 23, 2004 - v2
Checksum:i20986E236DBA270E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 4848MTDLT…KKPEW → MSRE1 Publication
Add
BLAST
Sequence conflicti137 – 1371K → KLK1 Publication
Sequence conflicti202 – 2021N → W in AAG38602. 1 Publication
Sequence conflicti215 – 2151P → T in AAG38602. 1 Publication
Sequence conflicti305 – 3062TH → SD in AAG38602. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF317284 Genomic DNA. Translation: AAG38602.1.
BX571965 Genomic DNA. Translation: CAH34402.1.
RefSeqiYP_107040.1. NC_006350.1.

Genome annotation databases

EnsemblBacteriaiCAH34402; CAH34402; BPSL0414.
GeneIDi3091431.
KEGGibps:BPSL0414.
PATRICi19260465. VBIBurPse99623_0464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF317284 Genomic DNA. Translation: AAG38602.1 .
BX571965 Genomic DNA. Translation: CAH34402.1 .
RefSeqi YP_107040.1. NC_006350.1.

3D structure databases

ProteinModelPortali Q9EYP3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272560.BPSL0414.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH34402 ; CAH34402 ; BPSL0414 .
GeneIDi 3091431.
KEGGi bps:BPSL0414.
PATRICi 19260465. VBIBurPse99623_0464.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi FERIATN.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci BPSE272560:GJNI-421-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Random sequencing of Burkholderia pseudomallei strain G9313 for clinical PCR development."
    Steiner B.M., Meyer R., Bowen M., Morrill W.
    Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: G9313.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K96243.

Entry informationi

Entry nameiLIPA_BURPS
AccessioniPrimary (citable) accession number: Q9EYP3
Secondary accession number(s): Q63XX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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