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Q9EYJ6

- MDH_RHIME

UniProt

Q9EYJ6 - MDH_RHIME

Protein

Malate dehydrogenase

Gene

mdh

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADBy similarity
    Binding sitei83 – 831SubstrateBy similarity
    Binding sitei89 – 891SubstrateBy similarity
    Binding sitei96 – 961NADBy similarity
    Binding sitei121 – 1211SubstrateBy similarity
    Binding sitei152 – 1521SubstrateBy similarity
    Active sitei176 – 1761Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 156NADBy similarity
    Nucleotide bindingi119 – 1213NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciSMEL266834:GJF6-3133-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:R03056
    ORF Names:SMc02479
    OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
    Taxonomic identifieri266834 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
    ProteomesiUP000001976: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Malate dehydrogenasePRO_0000113463Add
    BLAST

    Proteomic databases

    ProMEXiQ9EYJ6.

    Interactioni

    Protein-protein interaction databases

    STRINGi266834.SMc02479.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9EYJ6.
    SMRiQ9EYJ6. Positions 1-311.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213794.
    KOiK00024.
    OMAiYAPSAFV.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9EYJ6-1 [UniParc]FASTAAdd to Basket

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    MARNKIALIG SGMIGGTLAH LAGLKELGDI VLFDIADGIP QGKGLDIAQS    50
    SPVEGFDASL TGASDYSAIE GADVCIVTAG VPRKPGMSRD DLLGINLKVM 100
    EQVGAGIKKY APNAFVICIT NPLDAMVWAL QKFSGLPKNK VVGMAGVLDS 150
    SRFRLFLAEE FNVSVKDITA FVLGGHGDTM VPLARYSTVA GIPLPDLIQM 200
    GWTTKEKLDQ IIQRTRDGGA EIVGLLKTGS AYYAPAASAI EMAEAYLKDK 250
    KRVLPCAAHL SGQYGVKDMY VGVPTVIGAG GIERIIEIDL NKGEKEAFDK 300
    SVAAVAGLCE ACINIAPSLK 320
    Length:320
    Mass (Da):33,611
    Last modified:March 1, 2001 - v1
    Checksum:i3CF1514C7211F9FA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322647 Genomic DNA. Translation: AAG41996.1.
    AL591688 Genomic DNA. Translation: CAC47635.1.
    RefSeqiNP_387162.1. NC_003047.1.
    WP_003530257.1. NC_003047.1.

    Genome annotation databases

    EnsemblBacteriaiCAC47635; CAC47635; SMc02479.
    GeneIDi1234740.
    KEGGisme:SMc02479.
    PATRICi23635734. VBISinMel96828_4590.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF322647 Genomic DNA. Translation: AAG41996.1 .
    AL591688 Genomic DNA. Translation: CAC47635.1 .
    RefSeqi NP_387162.1. NC_003047.1.
    WP_003530257.1. NC_003047.1.

    3D structure databases

    ProteinModelPortali Q9EYJ6.
    SMRi Q9EYJ6. Positions 1-311.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 266834.SMc02479.

    Proteomic databases

    ProMEXi Q9EYJ6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC47635 ; CAC47635 ; SMc02479 .
    GeneIDi 1234740.
    KEGGi sme:SMc02479.
    PATRICi 23635734. VBISinMel96828_4590.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213794.
    KOi K00024.
    OMAi YAPSAFV.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci SMEL266834:GJF6-3133-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a malate dehydrogenase mutant and genes encoding a putative TCA cycle operon of Sinorhizobium meliloti."
      Dymov S.I., Meek D.J., Driscoll B.T.
      Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1021.

    Entry informationi

    Entry nameiMDH_RHIME
    AccessioniPrimary (citable) accession number: Q9EYJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3