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Q9EY50

- Q9EY50_WEIVI

UniProt

Q9EY50 - Q9EY50_WEIVI

Protein
Submitted name:

FemX

Gene

femX

Organism
Weissella viridescens (Lactobacillus viridescens)
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 53 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei37 – 371Important for catalytic activityImported
    Binding sitei71 – 711UDPImported
    Active sitei109 – 1091Proton donor/acceptorImported
    Binding sitei212 – 2121UDPImported
    Sitei212 – 2121Important for catalytic activityImported
    Active sitei320 – 3201Proton donor/acceptorImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi37 – 404UDPImported
    Nucleotide bindingi104 – 1074UDPImported

    GO - Molecular functioni

    1. transferase activity, transferring amino-acyl groups Source: InterPro

    Keywords - Ligandi

    Nucleotide-bindingImported

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15493.
    SABIO-RKQ9EY50.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    FemXImported
    Gene namesi
    Name:femXImported
    OrganismiWeissella viridescens (Lactobacillus viridescens)Imported
    Taxonomic identifieri1629 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLeuconostocaceaeWeissella

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NE9X-ray1.70A2-336[»]
    1P4NX-ray1.90A2-336[»]
    1XE4X-ray1.95A2-336[»]
    1XF8X-ray1.90A2-336[»]
    1XIXX-ray2.00A2-336[»]
    3GKRX-ray1.60A1-336[»]
    4II9X-ray1.66A1-336[»]
    ProteinModelPortaliQ9EY50.
    SMRiQ9EY50. Positions 2-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9EY50.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.630.30. 2 hits.
    InterProiIPR016181. Acyl_CoA_acyltransferase.
    IPR003447. FEMABX.
    [Graphical view]
    PfamiPF02388. FemAB. 2 hits.
    [Graphical view]
    SUPFAMiSSF55729. SSF55729. 2 hits.
    PROSITEiPS51191. FEMABX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9EY50-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVLNLNDPQ AVERYEEFMR QSPYGQVTQD LGWAKVKNNW EPVDVYLEDD    50
    QGAIIAAMSM LLGDTPTDKK FAYASKGPVM DVTDVDLLDR LVDEAVKALD 100
    GRAYVLRFDP EVAYSDEFNT TLQDHGYVTR NRNVADAGMH ATIQPRLNMV 150
    LDLTKFPDAK TTLDLYPSKT KSKIKRPFRD GVEVHSGNSA TELDEFFKTY 200
    TTMAERHGIT HRPIEYFQRM QAAFDADTMR IFVAEREGKL LSTGIALKYG 250
    RKIWYMYAGS MDGNTYYAPY AVQSEMIQWA LDTNTDLYDL GGIESESTDD 300
    SLYVFKHVFV KDAPREYIGE IDKVLDPEVY AELVKD 336
    Length:336
    Mass (Da):38,284
    Last modified:March 1, 2001 - v1
    Checksum:iAFB94A2718148C3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY008262 Genomic DNA. Translation: AAG21689.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY008262 Genomic DNA. Translation: AAG21689.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NE9 X-ray 1.70 A 2-336 [» ]
    1P4N X-ray 1.90 A 2-336 [» ]
    1XE4 X-ray 1.95 A 2-336 [» ]
    1XF8 X-ray 1.90 A 2-336 [» ]
    1XIX X-ray 2.00 A 2-336 [» ]
    3GKR X-ray 1.60 A 1-336 [» ]
    4II9 X-ray 1.66 A 1-336 [» ]
    ProteinModelPortali Q9EY50.
    SMRi Q9EY50. Positions 2-336.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15493.
    SABIO-RK Q9EY50.

    Miscellaneous databases

    EvolutionaryTracei Q9EY50.

    Family and domain databases

    Gene3Di 3.40.630.30. 2 hits.
    InterProi IPR016181. Acyl_CoA_acyltransferase.
    IPR003447. FEMABX.
    [Graphical view ]
    Pfami PF02388. FemAB. 2 hits.
    [Graphical view ]
    SUPFAMi SSF55729. SSF55729. 2 hits.
    PROSITEi PS51191. FEMABX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "FemABX family members are novel nonribosomal peptidyltransferases and important pathogen-specific drug targets."
      Hegde S.S., Shrader T.E.
      J. Biol. Chem. 276:6998-7003(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition."
      Biarrotte-Sorin S., Maillard A.P., Delettre J., Sougakoff W., Arthur M., Mayer C.
      Structure 12:257-267(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 2-336, ACTIVE SITE.
    3. "Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens."
      Maillard A.P., Biarrotte-Sorin S., Villet R., Mesnage S., Bouhss A., Sougakoff W., Mayer C., Arthur M.
      J. Bacteriol. 187:3833-3838(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 2-336.
    4. "Structure of Weissella viridescens FemX with the UDP-MurNAc-hexapeptide product of the alanine transfer reaction."
      Delfosse V., Piton J., Villet R., Lecerf M., Arthur M., Mayer C.
      Submitted (MAR-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
    5. "The structure of FemX(Wv) in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA(Ala) to peptidoglycan precursors."
      Fonvielle M., Li de La Sierra-Gallay I., El-Sagheer A.H., Lecerf M., Patin D., Mellal D., Mayer C., Blanot D., Gale N., Brown T., van Tilbeurgh H., Etheve-Quelquejeu M., Arthur M.
      Angew. Chem. Int. Ed. 52:7278-7281(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) IN COMPLEX WITH UDP.

    Entry informationi

    Entry nameiQ9EY50_WEIVI
    AccessioniPrimary (citable) accession number: Q9EY50
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3