Q9EXD8 (ULAB_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ascorbate-specific phosphotransferase enzyme IIB component
EC=2.7.1.69

Alternative name(s):
Ascorbate-specific PTS system EIIB component

Gene names

Name:	ulaB
Synonyms:	sgaB
Ordered Locus Names:	MPN_495
ORF Names:	MP347.1

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in ascorbate transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cytoplasm Potential.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Ascorbate-specific phosphotransferase enzyme IIB component		PRO_0000186688
Regions
	Domain	1 – 95	95	PTS EIIB type-2
Sites
	Active site	12	1	Phosphocysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9EXD8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 486BF2F3E2E4AEA9	FASTA	95	10,327
	10 20 30 40 50 60 MENKNLHIIA ACGNGMGTSM LIKIKVEKIM KELGYTAKVE ALSMGQTKGM EHSADIIISS 70 80 90 IHLTSEFNPN AKAKIVGVLN LMDENEIKQA LSKVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Re-annotating the Mycoplasma pneumoniae genome sequence: adding value, function and reading frames." Dandekar T., Huynen M., Regula J.T., Ueberle B., Zimmermann C.U., Andrade M.A., Doerks T., Sanchez-Pulido L., Snel B., Suyama M., Yuan Y.P., Herrmann R., Bork P. Nucleic Acids Res. 28:3278-3288(2000) [PubMed: 10954595] [Abstract] Cited for: IDENTIFICATION. Strain: ATCC 29342 / M129.
[3]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAG34743.1.
RefSeq	NP_110183.1. NC_000912.1.
3D structure databases
ProteinModelPortal	Q9EXD8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9EXD8. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877366.
GenomeReviews	Gene locus MPN_495 in contig U00089_GR.
KEGG	mpn:MPN495.
PATRIC	20022416. VBIMycPne110_0535.
Phylogenomic databases
HOGENOM	HBG757130.
OMA	MEHSADI.
PhylomeDB	Q9EXD8.
ProtClustDB	CLSK502319.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN495-MONOMER.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. [Graphical view]
KO	K02822.
Pfam	PF02302. PTS_IIB. 1 hit. [Graphical view]
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ULAB_MYCPN

Accession

Primary (citable) accession number: Q9EXD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program