Reviewed, UniProtKB/Swiss-Prot Q9EWS0 (KPRH_STRCO)

Names and origin

Protein names

Recommended name:
    Putative ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Ordered Locus Names:	SCO0782
ORF Names:	3SCF60.14c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Caution	This protein has lost two of the potential magnesium binding sites found in 5-phosphoribose 1-diphosphate synthetases. It is also divergent from other prokaryotic member of the family. It may therefore have acquired another function.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Putative ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141232
Regions
	Region	211 – 224	14	Binding of phosphoribosylpyrophosphate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9EWS0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BB117B7399811204	FASTA	317	33,959
	10 20 30 40 50 60 MRDIAVFSGS AHPDLAEEVC AQLGVPLSPT RVSRFANDCL EVQLMANCRE RDVFLVQPLV 70 80 90 100 110 120 TPVQEHLVEL LMMCDAARGA SAGRITVVMP HYSYARSDKK DAPRISLGGR LVADLLVAAG 130 140 150 160 170 180 ASRVLAMTLH SPQVHGFFSV PVDHLHALRE LAAHFRQYDL SRATVVSPDL GNAKEAAAFA 190 200 210 220 230 240 RMLGAQVAAG AKQRYADDRV SISSVIGDVA GRDVIVLDDE IAKGSTVLEL LDRLRESGPR 250 260 270 280 290 300 TIRLACTHGL FAAGALGRLS EQPDVLEIVC TNTVPVPADD HTDKLRILSI APALAEAVRR 310 IHNGESVSAL FDARPAG

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939106 Genomic DNA. Translation: CAC14347.1.
RefSeq	NP_625084.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	1096205.
GenomeReviews	Gene locus SCO0782 in contig AL645882_GR.
KEGG	sco:SCO0782.
NMPDR	fig|100226.1.peg.750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9EWS0.
OMA	NDCLEVQ.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO0782-MON.
BRENDA	2.7.6.1. 1084.
Family and domain databases
HAMAP	MF_00583. Divergent sequence. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRH_STRCO

Accession

Primary (citable) accession number: Q9EWS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)