Q9EWK0 (HIS2_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosyl-ATP pyrophosphatase
Short name=PRA-PH
EC=3.6.1.31

Gene names

Name:	hisE
Ordered Locus Names:	SCO1439
ORF Names:	SC6D7A.02c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-ATP + H2O = 1-(5-phosphoribosyl)-AMP + diphosphate. HAMAP MF_01020
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9. HAMAP MF_01020
Subcellular location	Cytoplasm By similarity HAMAP MF_01020.
Sequence similarities	Belongs to the PRA-PH family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosyl-ATP diphosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 90

90

Phosphoribosyl-ATP pyrophosphatase HAMAP MF_01020

PRO_0000136388

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

90

Helix Strand Turn

Details...

Helix

6 – 16

11

Helix

30 – 33

4

Helix

35 – 55

21

Helix

58 – 78

21

Helix

83 – 89

7

Sequences

	Sequence		Length	Mass (Da)
	Q9EWK0 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 50CACC42A8D97F55	FASTA	90	9,919
	10 20 30 40 50 60 MSKKTFEELF TELQHKAANG DPATSRTAEL VDKGVHAIGK KVVEEAAEVW MAAEYEGKDA 70 80 90 AAEEISQLLY HVQVMMVARG ISLDDVYAHL

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL939108 Genomic DNA. Translation: CAC17557.1.

RefSeq

NP_625720.1. NC_003888.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YXB	X-ray	2.60	A/B/C/D/E/F/G/H	1-90	[»]

ProteinModelPortal

Q9EWK0.

SMR

Q9EWK0. Positions 4-90.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1096865.

GenomeReviews

Gene locus SCO1439 in contig AL645882_GR.

KEGG

sco:SCO1439.

NMPDR

fig|100226.1.peg.1406.

PATRIC

23732456. VBIStrCoe124346_1449.

Phylogenomic databases

HOGENOM

HBG646527.

OMA

VWMAAEY.

PhylomeDB

Q9EWK0.

ProtClustDB

PRK00400.

Family and domain databases

HAMAP

MF_01020. HisE.
[Tree]

InterPro

IPR008179. PRib-ATP_PPHydrolase.
IPR021130. PRib-ATP_PPHydrolase-like.
[Graphical view]

KO

K01523.

Pfam

PF01503. PRA-PH. 1 hit.
[Graphical view]

TIGRFAMs

TIGR03188. Histidine_hisI. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HIS2_STRCO

Accession

Primary (citable) accession number: Q9EWK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program