Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerase subunit omega

Gene

rpoZ

Organism
Thermus aquaticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Transcription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit omega (EC:2.7.7.6)
Short name:
RNAP omega subunit
Alternative name(s):
RNA polymerase omega subunit
Transcriptase subunit omega
Gene namesi
Name:rpoZ
OrganismiThermus aquaticus
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 9998DNA-directed RNA polymerase subunit omegaPRO_0000129002Add
BLAST

Interactioni

Subunit structurei

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_5016.

Structurei

Secondary structure

1
99
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 116Combined sources
Beta strandi13 – 164Combined sources
Helixi17 – 3216Combined sources
Turni33 – 375Combined sources
Turni42 – 443Combined sources
Beta strandi51 – 533Combined sources
Helixi60 – 689Combined sources
Turni69 – 713Combined sources
Beta strandi73 – 786Combined sources
Helixi82 – 854Combined sources
Turni86 – 894Combined sources
Beta strandi91 – 933Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30E1-99[»]
1I6VX-ray3.30E1-99[»]
1L9UX-ray4.00E/N1-99[»]
1L9ZX-ray6.50E1-99[»]
1YNJX-ray3.20K1-99[»]
1YNNX-ray3.30K1-99[»]
4XLNX-ray4.00E/K1-99[»]
4XLPX-ray4.00E/K1-99[»]
4XLQX-ray4.60E/K1-99[»]
4XLRX-ray4.30E/K1-99[»]
4XLSX-ray4.01E/K1-99[»]
ProteinModelPortaliQ9EVV4.
SMRiQ9EVV4. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9EVV4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG41079SJ. Bacteria.
COG1758. LUCA.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
HAMAPiMF_00366. RNApol_bact_RpoZ.
InterProiIPR003716. DNA-dir_RNA_pol_omega.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
[Graphical view]
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
TIGRFAMsiTIGR00690. rpoZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EVV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPGIDKLF GMVDSKYRLT VVVAKRAQQL LRHRFKNTVL EPEERPKMRT
60 70 80 90
LEGLYDDPNA VTWAMKELLT GRLFFGENLV PEDRLQKEME RLYPTEEEA
Length:99
Mass (Da):11,625
Last modified:January 23, 2007 - v3
Checksum:i1BEC4908DB29CBF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295839 Genomic DNA. Translation: CAC15848.1.
RefSeqiWP_053767593.1. NZ_LHCI01000106.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295839 Genomic DNA. Translation: CAC15848.1.
RefSeqiWP_053767593.1. NZ_LHCI01000106.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQMX-ray3.30E1-99[»]
1I6VX-ray3.30E1-99[»]
1L9UX-ray4.00E/N1-99[»]
1L9ZX-ray6.50E1-99[»]
1YNJX-ray3.20K1-99[»]
1YNNX-ray3.30K1-99[»]
4XLNX-ray4.00E/K1-99[»]
4XLPX-ray4.00E/K1-99[»]
4XLQX-ray4.60E/K1-99[»]
4XLRX-ray4.30E/K1-99[»]
4XLSX-ray4.01E/K1-99[»]
ProteinModelPortaliQ9EVV4.
SMRiQ9EVV4. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi498848.TaqDRAFT_5016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG41079SJ. Bacteria.
COG1758. LUCA.

Miscellaneous databases

EvolutionaryTraceiQ9EVV4.

Family and domain databases

Gene3Di3.90.940.10. 1 hit.
HAMAPiMF_00366. RNApol_bact_RpoZ.
InterProiIPR003716. DNA-dir_RNA_pol_omega.
IPR006110. Pol_omega/K/RPB6.
IPR012293. RNAP_RPB6_omega.
[Graphical view]
PfamiPF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SMARTiSM01409. RNA_pol_Rpb6. 1 hit.
[Graphical view]
SUPFAMiSSF63562. SSF63562. 1 hit.
TIGRFAMsiTIGR00690. rpoZ. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Recombinant Thermus aquaticus RNA polymerase, a new tool for structure-based analysis of transcription."
    Minakhin L., Nechaev S., Campbell E.A., Severinov K.
    J. Bacteriol. 183:71-76(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly."
    Minakhin L., Bhagat S., Brunning A., Campbell E.A., Darst S.A., Ebright R.H., Severinov K.
    Proc. Natl. Acad. Sci. U.S.A. 98:892-897(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, 3D-STRUCTURE MODELING.

Entry informationi

Entry nameiRPOZ_THEAQ
AccessioniPrimary (citable) accession number: Q9EVV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.