Q9EVV4 (RPOZ_THEAQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit omega
Short name=RNAP omega subunit
EC=2.7.7.6

Alternative name(s):
RNA polymerase omega subunit
Transcriptase subunit omega

Gene names

Name:

rpoZ

Organism

Thermus aquaticus

Taxonomic identifier

271 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	99 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits. Ref.2
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_00366
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Sequence similarities	Belongs to the RNA polymerase subunit omega family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	RNA polymerase complex Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 99

98

DNA-directed RNA polymerase subunit omega HAMAP MF_00366

PRO_0000129002

Secondary structure

1

.

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.

.

99

Helix Strand Turn

Details...

Helix

6 – 11

6

Beta strand

13 – 16

4

Helix

17 – 32

16

Turn

42 – 44

3

Beta strand

51 – 53

3

Helix

60 – 68

9

Turn

69 – 71

3

Beta strand

73 – 78

6

Helix

82 – 85

4

Turn

86 – 89

4

Sequences

	Sequence		Length	Mass (Da)
	Q9EVV4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1BEC4908DB29CBF6	FASTA	99	11,625
	10 20 30 40 50 60 MAEPGIDKLF GMVDSKYRLT VVVAKRAQQL LRHRFKNTVL EPEERPKMRT LEGLYDDPNA 70 80 90 VTWAMKELLT GRLFFGENLV PEDRLQKEME RLYPTEEEA

References

[1]	"Recombinant Thermus aquaticus RNA polymerase, a new tool for structure-based analysis of transcription." Minakhin L., Nechaev S., Campbell E.A., Severinov K. J. Bacteriol. 183:71-76(2001) [PubMed: 11114902] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly." Minakhin L., Bhagat S., Brunning A., Campbell E.A., Darst S.A., Ebright R.H., Severinov K. Proc. Natl. Acad. Sci. U.S.A. 98:892-897(2001) [PubMed: 11158566] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-11, FUNCTION, 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ295839 Genomic DNA. Translation: CAC15848.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HQM	X-ray	3.30	E	1-99	[»]
1I6V	X-ray	3.30	E	1-99	[»]
1L9U	X-ray	4.00	E/N	1-99	[»]
1L9Z	X-ray	6.50	E	1-99	[»]
1YNJ	X-ray	3.20	K	1-99	[»]
1YNN	X-ray	3.30	K	1-99	[»]

ProteinModelPortal

Q9EVV4.

SMR

Q9EVV4. Positions 1-98.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_00366. RNApol_bact_RpoZ.
[Tree]

InterPro

IPR003716. DNA-dir_RNA_pol_omega.
IPR006110. RNA_poly_omega/K/RPABC2.
IPR012293. RNAP_RPB6_omega.
[Graphical view]

Gene3D

G3DSA:3.90.940.10. RNAP_RPB6_omega. 1 hit.

Pfam

PF01192. RNA_pol_Rpb6. 1 hit.
[Graphical view]

SUPFAM

SSF63562. RNA_pol_omega. 1 hit.

TIGRFAMs

TIGR00690. RpoZ. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RPOZ_THEAQ

Accession

Primary (citable) accession number: Q9EVV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 4, 2001
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 69 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program