3-isopropylmalate dehydrogenase - Buchnera aphidicola subsp. Uroleucon ambrosiae

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Reviewed, UniProtKB/Swiss-Prot Q9EVG9 (LEU3_BUCUM)

Last modified June 16, 2009. Version 48. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:

leuB

Encoded on

Plasmid pLeu (pBAp1)

Organism

Buchnera aphidicola subsp. Uroleucon ambrosiae

Taxonomic identifier

118117 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD⁺ = 4-methyl-2-oxopentanoate + CO₂ + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

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Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 365

365

3-isopropylmalate dehydrogenase HAMAP MF_01033

PRO_0000083664

Regions

Nucleotide binding

78 – 91

NAD By similarity

Nucleotide binding

287 – 299

NAD By similarity

Sites

Metal binding

229

Magnesium or manganese By similarity

Metal binding

253

Magnesium or manganese By similarity

Metal binding

257

Magnesium or manganese By similarity

Binding site

Substrate By similarity

Binding site

109

Substrate By similarity

Binding site

139

Substrate By similarity

Binding site

229

Substrate By similarity

Site

146

Important for catalysis By similarity

Site

197

Important for catalysis By similarity

Natural variations

Natural variant

255

V → I in strain: GA2181.

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9EVG9-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 14F1E76B771C5764
Show »

FASTA

365

40,759

        10         20         30         40         50         60 
MKTKYRISVL PGDGIGPEVM REAYKILNIL KNHFSLPLEI KEFNIGGAAI DQEGVALPKK 

        70         80         90        100        110        120 
TLLGCENSDA ILFGSVGGKK WDHLPIDKRP ERASLLPLRK HFNLFANLRP AQLYSELKYL 

       130        140        150        160        170        180 
SPLRSDIXIK NGFNILCIRE LTGGIYFGKP TGRXLKKNNI EYAFDTEIYY DYEINRIAHL 

       190        200        210        220        230        240 
AFQLAQTRNY KVCSIDKSNV LNSSVLWRET VQKVSKNYPD VHLSHLYIDN ATMQIIKDPN 

       250        260        270        280        290        300 
QFDILLCSNL FGDIVSDECA IITGSIGMLP SASLNEKKFG LYEPAGGSAP DIEGKNIANP 

       310        320        330        340        350        360 
IAQILSVSML VRYGMNLKKI ADKIDQSVIS VLKKGYRTAD ISNNNNYLKT NEMGDVIANT 


LISGE

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References

[1]

"Vertical transmission of biosynthetic plasmids in aphid endosymbionts (Buchnera)."
Wernegreen J.J., Moran N.A.
J. Bacteriol. 183:785-790(2001) [PubMed: 11133977] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

[2]

"Intraspecific variation in symbiont genomes: bottlenecks and the aphid-Buchnera association."
Funk D.J., Wernegreen J.J., Moran N.A.
Genetics 157:477-489(2001) [PubMed: 11156972] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 245-365.
Strain: AL.012, AZ.023, AZ.026, AZ.065, AZ.180, DC.005, GA.039, GA2181, IL.014, IL2.17, IN.018, KY.172, KY2.37, LA.013, MI.035, MN.001, MNb027, MS.040, NY.016, OH.036, TN.173, TN2.38, UT.002 and VA.015.

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Cross-references

Sequence databases
	AF197454 Genomic DNA. Translation: AAG31396.1. AF196403 Genomic DNA. Translation: AAK21724.1. AF196404 Genomic DNA. Translation: AAK21725.1. AF196407 Genomic DNA. Translation: AAK21730.1. AF196408 Genomic DNA. Translation: AAK21732.1. AF196410 Genomic DNA. Translation: AAK21736.1. AF196412 Genomic DNA. Translation: AAK21740.1. AF196413 Genomic DNA. Translation: AAK21742.1. AF196414 Genomic DNA. Translation: AAK21744.1. AF196415 Genomic DNA. Translation: AAK21746.1. AF196417 Genomic DNA. Translation: AAK21750.1. AF196418 Genomic DNA. Translation: AAK21752.1. AF196419 Genomic DNA. Translation: AAK21754.1. AF196420 Genomic DNA. Translation: AAK21756.1. AF196421 Genomic DNA. Translation: AAK21758.1. AF196422 Genomic DNA. Translation: AAK21760.1. AF196423 Genomic DNA. Translation: AAK21762.1. AF196424 Genomic DNA. Translation: AAK21764.1. AF196402 Genomic DNA. Translation: AAK21722.1. AF196406 Genomic DNA. Translation: AAK21728.1. AF196416 Genomic DNA. Translation: AAK21748.1. AF196425 Genomic DNA. Translation: AAK21766.1. AF196409 Genomic DNA. Translation: AAK21734.1. AF196411 Genomic DNA. Translation: AAK21738.1. AF196405 Genomic DNA. Translation: AAK21727.1.
3D structure databases
HSSP	HSSP built from PDB template 1CNZ based on UniProtKB P37412.
ModBase	Search...
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

LEU3_BUCUM

Accession

Primary (citable) accession number: Q9EVG9
Secondary accession number(s): Q99Q96

Q9AJ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Family and domain databases

Entry information

Relevant documents