Reviewed, UniProtKB/Swiss-Prot Q9EV38 (LACG_STRGC)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-galactosidase
    EC=3.2.1.85
Alternative name(s):
    Beta-D-phosphogalactoside galactohydrolase
      Short name=PGALase
    P-beta-Gal
      Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SGO_1512

Organism

Streptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]

Taxonomic identifier

29390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro galactosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260729
Sites
	Active site	160	1	Proton donor By similarity
	Active site	375	1	Nucleophile By similarity
Experimental info
	Sequence conflict	459 – 460	2	RR → KK in AAG39001. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9EV38-1 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 86A4A0301682873E	FASTA	468	54,111
	10 20 30 40 50 60 MTKSLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYHKYPVDL 70 80 90 100 110 120 KLAEEYGVNG IRISIAWSRI FPTGYGEVNP KGVEFYHNLF AECHKRHVEP FVTLHHFDTP 130 140 150 160 170 180 EALHSNGDFL NRDNIEHFVD YAAFCFEEFP EVRYWTTFNE IGPIGDGQYL VGKFPPGIQY 190 200 210 220 230 240 DLAKVFQSHH NMMVSHARAV KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI 250 260 270 280 290 300 IHNKFILDAT YLGHYSDVTL AGVNHILKVN GGQLDLRDED FAALEAAKDL NDFLGINYYM 310 320 330 340 350 360 SDWMRDFDGE TEIIHNGKGE KGSSKYQIKG VGRRESPTHI PKTDWDWIIY PQGLYDQIMR 370 380 390 400 410 420 IKKDYPNYKK IYITENGLGY KDEFVDNTVY DDARIDYVKQ HLEVLSDAIA DGANVKGYFI 430 440 450 460 WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAHWYRR LAETQMIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of new intergenic and intragenic integration sites for foreign gene expression in recombinant Streptococcus gordonii strains." Bolken T.C., Franke C.A., Hruby D.E., Zeller G.O. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide." Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R. J. Bacteriol. 189:7799-7807(2007) [PubMed: 17720781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF210773 Genomic DNA. Translation: AAG39001.1. CP000725 Genomic DNA. Translation: ABV10357.1.
RefSeq	YP_001450791.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
SMR	Q9EV38. Positions 1-468.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	5598019.
GenomeReviews	Gene locus SGO_1512 in contig CP000725_GR.
KEGG	sgo:SGO_1512.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG316462.
OMA	LYKEKGY.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR005928. LacG. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
TIGRFAMs	TIGR01233. lacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_STRGC

Accession

Primary (citable) accession number: Q9EV38
Secondary accession number(s): A8AYD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)