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Q9EV38 (LACG_STRGC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-galactosidase

EC=3.2.1.85
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG
Gene names
Name:lacG
Ordered Locus Names:SGO_1512
OrganismStreptococcus gordonii (strain Challis / ATCC 35105 / CH1 / DL1 / V288) [Complete proteome] [HAMAP]
Taxonomic identifier29390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP-Rule MF_01574

Pathway

Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP-Rule MF_01574

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlactose catabolic process via tagatose-6-phosphate

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phospho-beta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4684686-phospho-beta-galactosidase HAMAP-Rule MF_01574
PRO_0000260729

Sites

Active site1601Proton donor By similarity
Active site3751Nucleophile By similarity

Experimental info

Sequence conflict459 – 4602RR → KK in AAG39001. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9EV38 [UniParc].

Last modified February 26, 2008. Version 2.
Checksum: 86A4A0301682873E

FASTA46854,111
        10         20         30         40         50         60 
MTKSLPKDFI FGGATAAYQA EGATHTDGKG PVAWDKYLED NYWYTAEPAS DFYHKYPVDL 

        70         80         90        100        110        120 
KLAEEYGVNG IRISIAWSRI FPTGYGEVNP KGVEFYHNLF AECHKRHVEP FVTLHHFDTP 

       130        140        150        160        170        180 
EALHSNGDFL NRDNIEHFVD YAAFCFEEFP EVRYWTTFNE IGPIGDGQYL VGKFPPGIQY 

       190        200        210        220        230        240 
DLAKVFQSHH NMMVSHARAV KLYKDKGYKG EIGVVHALPT KYPYDPENPA DVRAAELEDI 

       250        260        270        280        290        300 
IHNKFILDAT YLGHYSDVTL AGVNHILKVN GGQLDLRDED FAALEAAKDL NDFLGINYYM 

       310        320        330        340        350        360 
SDWMRDFDGE TEIIHNGKGE KGSSKYQIKG VGRRESPTHI PKTDWDWIIY PQGLYDQIMR 

       370        380        390        400        410        420 
IKKDYPNYKK IYITENGLGY KDEFVDNTVY DDARIDYVKQ HLEVLSDAIA DGANVKGYFI 

       430        440        450        460 
WSLMDVFSWS NGYEKRYGLF YVDFETQERY PKKSAHWYRR LAETQMIE 

« Hide

References

« Hide 'large scale' references
[1]"Identification of new intergenic and intragenic integration sites for foreign gene expression in recombinant Streptococcus gordonii strains."
Bolken T.C., Franke C.A., Hruby D.E., Zeller G.O.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide."
Vickerman M.M., Iobst S., Jesionowski A.M., Gill S.R.
J. Bacteriol. 189:7799-7807(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Challis / ATCC 35105 / CH1 / DL1 / V288.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF210773 Genomic DNA. Translation: AAG39001.1.
CP000725 Genomic DNA. Translation: ABV10357.1.
RefSeqYP_001450791.1. NC_009785.1.

3D structure databases

ProteinModelPortalQ9EV38.
SMRQ9EV38. Positions 1-468.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING467705.SGO_1512.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV10357; ABV10357; SGO_1512.
GeneID5598019.
KEGGsgo:SGO_1512.
PATRIC19660976. VBIStrGor124371_1489.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088632.
KOK01220.
OMAVKRFATF.
OrthoDBEOG60658J.
ProtClustDBPRK13511.

Enzyme and pathway databases

BioCycSGOR467705:GH3R-1511-MONOMER.
UniPathwayUPA00542; UER00605.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
HAMAPMF_01574. LacG.
InterProIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01233. lacG. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLACG_STRGC
AccessionPrimary (citable) accession number: Q9EV38
Secondary accession number(s): A8AYD1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: February 26, 2008
Last modified: February 19, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries