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Protein

Aspartate-semialdehyde dehydrogenase

Gene

asd

Organism
Yersinia pestis
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.UniRule annotation

Catalytic activityi

L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731NADPUniRule annotation
Binding sitei102 – 1021PhosphateUniRule annotation
Active sitei135 – 1351Acyl-thioester intermediateUniRule annotation
Binding sitei162 – 1621SubstrateUniRule annotation
Binding sitei193 – 1931NADP; via carbonyl oxygenUniRule annotation
Binding sitei241 – 2411SubstrateUniRule annotation
Binding sitei244 – 2441PhosphateUniRule annotation
Binding sitei267 – 2671SubstrateUniRule annotation
Active sitei274 – 2741Proton acceptorUniRule annotation
Binding sitei350 – 3501NADPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 134NADPUniRule annotation
Nucleotide bindingi37 – 382NADPUniRule annotation
Nucleotide bindingi165 – 1662NADPUniRule annotation

GO - Molecular functioni

  1. aspartate-semialdehyde dehydrogenase activity Source: UniProtKB-HAMAP
  2. N-acetyl-gamma-glutamyl-phosphate reductase activity Source: InterPro
  3. NAD binding Source: InterPro
  4. NADP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' L-methionine biosynthetic process Source: UniProtKB-HAMAP
  2. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  3. isoleucine biosynthetic process Source: InterPro
  4. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
  5. threonine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesisUniRule annotation, Lysine biosynthesisUniRule annotation, Methionine biosynthesisUniRule annotation, Threonine biosynthesisUniRule annotation

Keywords - Ligandi

NADPUniRule annotation

Enzyme and pathway databases

BioCyciYPES214092:GKDD-3924-MONOMER.
UniPathwayiUPA00034; UER00016.
UPA00050; UER00463.
UPA00051; UER00464.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate-semialdehyde dehydrogenaseUniRule annotation (EC:1.2.1.11UniRule annotation)
Short name:
ASA dehydrogenaseUniRule annotation
Short name:
ASADHUniRule annotation
Alternative name(s):
Aspartate-beta-semialdehyde dehydrogenaseUniRule annotation
Gene namesi
Name:asdUniRule annotationImported
Synonyms:asd2Imported
Ordered Locus Names:y3880Imported, YP_3311Imported, YPO3949Imported
OrganismiYersinia pestisImported
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000815 Componenti: Chromosome UP000001019 Componenti: Chromosome UP000002490 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi214092.YPO3949.

Family & Domainsi

Sequence similaritiesi

Belongs to the aspartate-semialdehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000161376.
KOiK00133.
OMAiMTSMTYQ.
OrthoDBiEOG67X1PS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_02121. ASADH.
InterProiIPR000319. Asp-semialdehyde_DH_CS.
IPR011534. Asp_ADH_gamma-type.
IPR012080. Asp_semialdehyde_DH.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFiPIRSF000148. ASA_dh. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01745. asd_gamma. 1 hit.
PROSITEiPS01103. ASD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ETB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNVGFIGWR GMVGSVLMQR MIEERDFDGI RPVFFSTSQH GQAAPAFAGH
60 70 80 90 100
QGTLQDAYDI DALSALDIII TCQGGDYTNQ IYPKLREIGW QGYWIDAASS
110 120 130 140 150
LRMQDDTIII LDPVNHDVIK QGLDKGIKTF AGGNCTVSLM LMSLGGLFAN
160 170 180 190 200
NLVEWASVAT YQAASGGGAR HMRELLTQMG MLHAGVAKEL QDPASAILDI
210 220 230 240 250
ERKVTSTTRS GTLPTDNFGV PLAGSLIPWI DKALDNGQSR EEWKGQAETN
260 270 280 290 300
KILSTSTLIP VDGLCVRVGA LRCHSQAFTL KLKKDVPLAE IEQMLATHND
310 320 330 340 350
WVRVIPNDRE LSMRELTPAA VTGTLNTPVG RLRKLNMGPE YLSAFTVGDQ
360
LLWGAAEPLR RMLRILL
Length:367
Mass (Da):40,191
Last modified:March 1, 2001 - v1
Checksum:iEFFEE5C4625B6DE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282318 Genomic DNA. Translation: AAG22007.1.
AE009952 Genomic DNA. Translation: AAM87425.1.
AE017042 Genomic DNA. Translation: AAS63476.1.
AL590842 Genomic DNA. Translation: CAL22530.1.
PIRiAG0480.
RefSeqiNP_671174.1. NC_004088.1.
NP_994599.1. NC_005810.1.
YP_002348820.1. NC_003143.1.

Genome annotation databases

EnsemblBacteriaiAAM87425; AAM87425; y3880.
AAS63476; AAS63476; YP_3311.
KGA52760; KGA52760; DJ56_772.
GeneIDi1176788.
KEGGiype:YPO3949.
ypk:y3880.
ypm:YP_3311.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282318 Genomic DNA. Translation: AAG22007.1.
AE009952 Genomic DNA. Translation: AAM87425.1.
AE017042 Genomic DNA. Translation: AAS63476.1.
AL590842 Genomic DNA. Translation: CAL22530.1.
PIRiAG0480.
RefSeqiNP_671174.1. NC_004088.1.
NP_994599.1. NC_005810.1.
YP_002348820.1. NC_003143.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi214092.YPO3949.

Protocols and materials databases

DNASUi1148827.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM87425; AAM87425; y3880.
AAS63476; AAS63476; YP_3311.
KGA52760; KGA52760; DJ56_772.
GeneIDi1176788.
KEGGiype:YPO3949.
ypk:y3880.
ypm:YP_3311.

Phylogenomic databases

HOGENOMiHOG000161376.
KOiK00133.
OMAiMTSMTYQ.
OrthoDBiEOG67X1PS.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00016.
UPA00050; UER00463.
UPA00051; UER00464.
BioCyciYPES214092:GKDD-3924-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_02121. ASADH.
InterProiIPR000319. Asp-semialdehyde_DH_CS.
IPR011534. Asp_ADH_gamma-type.
IPR012080. Asp_semialdehyde_DH.
IPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
IPR012280. Semialdhyde_DH_dimer_dom.
[Graphical view]
PfamiPF01118. Semialdhyde_dh. 1 hit.
PF02774. Semialdhyde_dhC. 1 hit.
[Graphical view]
PIRSFiPIRSF000148. ASA_dh. 1 hit.
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01745. asd_gamma. 1 hit.
PROSITEiPS01103. ASD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic relatedness of Yersinia."
    Lindler L.E., Popek M., Huang X.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: KIMImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar OrientalisImported and CO92Imported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KIMImported and KIM10+ / Biovar MediaevalisImported.
  4. "Annotation and evolutionary relationships of a small regulatory RNA gene micF and its target ompF in Yersinia species."
    Delihas N.
    BMC Microbiol. 3:13-13(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: CO92Imported.
  5. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001Imported and 91001 / Biovar MediaevalisImported.
  6. "Genetics of metabolic variations between Yersinia pestis biovars and the proposal of a new biovar, microtus."
    Zhou D., Tong Z., Song Y., Han Y., Pei D., Pang X., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R., Du Z., Wang J., Guo Z., Wang J., Huang P., Yang R.
    J. Bacteriol. 186:5147-5152(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 91001Imported.

Entry informationi

Entry nameiQ9ETB0_YERPE
AccessioniPrimary (citable) accession number: Q9ETB0
Secondary accession number(s): Q74QZ1, Q7CFY8
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.