Reviewed, UniProtKB/Swiss-Prot Q9ET64 (NSMA_RAT)

Names and origin

Protein names

Recommended name:
    Sphingomyelin phosphodiesterase 2
    EC=3.1.4.12
Alternative name(s):
    Neutral sphingomyelinase
      Short name=nSMase
      Short name=N-SMase
    Lyso-platelet-activating factor-phospholipase C
      Short name=Lyso-PAF-PLC

Gene names

Name:

Smpd2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	ceramide biosynthetic process Inferred from direct assay. Source: RGD protein kinase cascade Inferred from mutant phenotype. Source: RGD response to mechanical stimulus Inferred from mutant phenotype. Source: RGD
Cellular component	caveola Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from direct assay. Source: RGD
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Sphingomyelin phosphodiesterase 2		PRO_0000075688
Regions
	Transmembrane	325 – 345	21	Potential
	Transmembrane	354 – 374	21	Potential
Sites
	Active site	272	1	Proton acceptor Probable
	Metal binding	49	1	Magnesium By similarity
	Site	180	1	Important for substrate recognition By similarity
Experimental info
	Mutagenesis	136	1	H → A: Complete loss of activity.
	Mutagenesis	151	1	H → A: Reduced activity.
	Mutagenesis	151	1	H → Y: Complete loss of activity.
	Mutagenesis	272	1	H → A: Complete loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	Q9ET64-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 109A5133A056AAF1	FASTA	422	47,645
	10 20 30 40 50 60 MKHNFSLRLR VFNLNCWDIP YLSKHRADRM KRLGDFLNLE SFDLALLEEV WSEQDFQYLK 70 80 90 100 110 120 QKLSLTYPDA HYFRSGIIGS GLCVFSRHPI QEIVQHVYTL NGYPYKFYHG DWFCGKAVGL 130 140 150 160 170 180 LVLHLSGLVL NAYVTHLHAE YSRQKDIYFA HRVAQAWELA QFIHHTSKKA NVVLLCGDLN 190 200 210 220 230 240 MHPKDLGCCL LKEWTGLRDA FVETEDFKGS EDGCTMVPKN CYVSQQDLGP FPFGVRIDYV 250 260 270 280 290 300 LYKAVSGFHI CCKTLKTTTG CDPHNGTPFS DHEALMATLC VKHSPPQEDP CSAHGSAERS 310 320 330 340 350 360 ALISALREAR TELGRGIAQA RWWAALFGYV MILGLSLLVL LCVLAAGEEA REVAIMLWTP 370 380 390 400 410 420 SVGLVLGAGA VYLFHKQEAK SLCRAQAEIQ HVLTRTTETQ DLGSEPHPTH CRQQEADRAE EK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of rat neutral sphingomyelinase: enzymological characterization and identification of essential histidine residues." Mizutani Y., Tamiya-Koizumi K., Irie F., Hirabayashi Y., Miwa M., Yoshida S. Biochim. Biophys. Acta 1485:236-246(2000) [PubMed: 10832103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, MUTAGENESIS. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB047002 mRNA. Translation: BAB08219.1. BC091139 mRNA. Translation: AAH91139.1.
IPI	IPI00192440.
RefSeq	NP_112650.1.
UniGene	Rn.18572
3D structure databases
SMR	Q9ET64. Positions 9-279.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9ET64.
Genome annotation databases
Ensembl	ENSRNOT00000000336; ENSRNOP00000000336; ENSRNOG00000000306; Rattus norvegicus. [Genome view]
GeneID	83537.
KEGG	rno:83537.
UCSC	NM_031360. rat.
Organism-specific databases
CTD	83537.
RGD	619753. Smpd2.
Phylogenomic databases
eggNOG	roNOG07612.
HOVERGEN	Q9ET64.
InParanoid	Q9ET64.
OMA	DFQYLRQ.
OrthoDB	EOG9GJ28P.
PhylomeDB	Q9ET64.
Enzyme and pathway databases
BRENDA	3.1.4.12. 248.
Gene expression databases
ArrayExpress	Q9ET64.
Genevestigator	Q9ET64.
GermOnline	ENSRNOG00000000306. Rattus norvegicus.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	616049.

Entry information

Entry name

NSMA_RAT

Accession

Primary (citable) accession number: Q9ET64
Secondary accession number(s): Q5BKB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)