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Protein

Sphingomyelin phosphodiesterase 2

Gene

Smpd2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

Cofactori

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491MagnesiumBy similarity
Sitei180 – 1801Important for substrate recognitionBy similarity
Active sitei272 – 2721Proton acceptorCurated

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • sphingomyelin phosphodiesterase activity Source: RGD

GO - Biological processi

  • apoptotic signaling pathway Source: Reactome
  • ceramide biosynthetic process Source: RGD
  • intracellular signal transduction Source: RGD
  • response to mechanical stimulus Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.12. 5301.
ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
R-RNO-193681. Ceramide signalling.
R-RNO-5626978. TNFR1-mediated ceramide production.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesterase 2 (EC:3.1.4.12)
Alternative name(s):
Lyso-platelet-activating factor-phospholipase C
Short name:
Lyso-PAF-PLC
Neutral sphingomyelinase
Short name:
N-SMase
Short name:
nSMase
Gene namesi
Name:Smpd2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi619753. Smpd2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei325 – 34521HelicalSequence analysisAdd
BLAST
Transmembranei354 – 37421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • caveola Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • membrane Source: RGD
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi136 – 1361H → A: Complete loss of activity. 1 Publication
Mutagenesisi151 – 1511H → A: Reduced activity. 1 Publication
Mutagenesisi151 – 1511H → Y: Complete loss of activity. 1 Publication
Mutagenesisi272 – 2721H → A: Complete loss of activity. 1 Publication

Chemistry

ChEMBLiCHEMBL3528.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422Sphingomyelin phosphodiesterase 2PRO_0000075688Add
BLAST

Proteomic databases

PaxDbiQ9ET64.
PRIDEiQ9ET64.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000306.
GenevisibleiQ9ET64. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000336.

Chemistry

BindingDBiQ9ET64.

Structurei

3D structure databases

ProteinModelPortaliQ9ET64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the neutral sphingomyelinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3873. Eukaryota.
ENOG410ZFPY. LUCA.
GeneTreeiENSGT00390000009166.
HOGENOMiHOG000044683.
HOVERGENiHBG019089.
InParanoidiQ9ET64.
KOiK12351.
OMAiQARWWAT.
OrthoDBiEOG091G0EZ8.
PhylomeDBiQ9ET64.
TreeFamiTF313899.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ET64-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHNFSLRLR VFNLNCWDIP YLSKHRADRM KRLGDFLNLE SFDLALLEEV
60 70 80 90 100
WSEQDFQYLK QKLSLTYPDA HYFRSGIIGS GLCVFSRHPI QEIVQHVYTL
110 120 130 140 150
NGYPYKFYHG DWFCGKAVGL LVLHLSGLVL NAYVTHLHAE YSRQKDIYFA
160 170 180 190 200
HRVAQAWELA QFIHHTSKKA NVVLLCGDLN MHPKDLGCCL LKEWTGLRDA
210 220 230 240 250
FVETEDFKGS EDGCTMVPKN CYVSQQDLGP FPFGVRIDYV LYKAVSGFHI
260 270 280 290 300
CCKTLKTTTG CDPHNGTPFS DHEALMATLC VKHSPPQEDP CSAHGSAERS
310 320 330 340 350
ALISALREAR TELGRGIAQA RWWAALFGYV MILGLSLLVL LCVLAAGEEA
360 370 380 390 400
REVAIMLWTP SVGLVLGAGA VYLFHKQEAK SLCRAQAEIQ HVLTRTTETQ
410 420
DLGSEPHPTH CRQQEADRAE EK
Length:422
Mass (Da):47,645
Last modified:March 1, 2001 - v1
Checksum:i109A5133A056AAF1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047002 mRNA. Translation: BAB08219.1.
BC091139 mRNA. Translation: AAH91139.1.
RefSeqiNP_112650.1. NM_031360.2.
XP_008771229.1. XM_008773007.1.
UniGeneiRn.18572.

Genome annotation databases

EnsembliENSRNOT00000000336; ENSRNOP00000000336; ENSRNOG00000000306.
GeneIDi83537.
KEGGirno:83537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB047002 mRNA. Translation: BAB08219.1.
BC091139 mRNA. Translation: AAH91139.1.
RefSeqiNP_112650.1. NM_031360.2.
XP_008771229.1. XM_008773007.1.
UniGeneiRn.18572.

3D structure databases

ProteinModelPortaliQ9ET64.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000336.

Chemistry

BindingDBiQ9ET64.
ChEMBLiCHEMBL3528.

Proteomic databases

PaxDbiQ9ET64.
PRIDEiQ9ET64.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000336; ENSRNOP00000000336; ENSRNOG00000000306.
GeneIDi83537.
KEGGirno:83537.

Organism-specific databases

CTDi6610.
RGDi619753. Smpd2.

Phylogenomic databases

eggNOGiKOG3873. Eukaryota.
ENOG410ZFPY. LUCA.
GeneTreeiENSGT00390000009166.
HOGENOMiHOG000044683.
HOVERGENiHBG019089.
InParanoidiQ9ET64.
KOiK12351.
OMAiQARWWAT.
OrthoDBiEOG091G0EZ8.
PhylomeDBiQ9ET64.
TreeFamiTF313899.

Enzyme and pathway databases

UniPathwayiUPA00222.
BRENDAi3.1.4.12. 5301.
ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
R-RNO-193681. Ceramide signalling.
R-RNO-5626978. TNFR1-mediated ceramide production.

Miscellaneous databases

PROiQ9ET64.

Gene expression databases

BgeeiENSRNOG00000000306.
GenevisibleiQ9ET64. RN.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNSMA_RAT
AccessioniPrimary (citable) accession number: Q9ET64
Secondary accession number(s): Q5BKB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.