Q9ET54 (PALLD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palladin

Gene names

Name:	Palld
Synonyms:	Kiaa0992

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1408 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure. Ref.4 Ref.9 Ref.10 Ref.13
Subunit structure	Interacts with ACTN, ARGBP2, LPP, PFN1, SPIN90, SRC and EZR By similarity. Interacts with EPS8, LASP1 and VASP. Ref.7 Ref.10 Ref.11
Subcellular location	Cytoplasm › cytoskeleton. Cell junction › focal adhesion. Cell projection › ruffle. Cell projection › lamellipodium. Cytoplasm › myofibril › sarcomere › Z line. Note: Localizes to stress fibers and Z lines. Ref.4
Tissue specificity	Detected in both muscle and non-muscle tissues and cells (at protein level). Isoform 3 is widely expressed, isoform 4 is particularly abundant in tissues rich in smooth muscle and in the cardiac muscle and isoform 1 is detected in heart. Ref.4 Ref.6
Developmental stage	Ubiquitously detected in embryonic tissues, and down-regulated in certain adult tissues (at protein level). Isoform 3 is widely expressed in embryonic tissues (at protein level). In adults is detected in spleen and gut, and is almost undetectable in heart, skeletal muscle, liver, and kidney (at protein level). Isoform 4 is widely expressed in neonatal tissues (brain, heart, lung, stomach, intestine, kidney, bone and skin) (at protein level). Late in development expression is restricted to cardiac muscle and to organs rich in smooth muscle (at protein level). Isoform 1 is detected in neonatal striated muscle and bone, and remains highly expressed in adult skeletal and cardiac muscle (at protein level). Adult brain express an isoform of 80-85 kDa. At E8.5 is mainly expressed the rostral and caudal part of neural plate. No expression is detected in somite. At E9.5 and E10.5 is ubiquitously detected. Ref.4 Ref.9
Post-translational modification	Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines. Phosphorylation at Ser-1143 by PKB/AKT1 modulates cytoskeletal organization and cell motility By similarity. Ref.4
Disruption phenotype	Death around E15.5 due to severe neural tube closure defects and herniation of liver and intestine. Palld-deficient mouse embryonic fibroblasts display disorganized actin cytoskeleton, decreased polymerized filament actin, and decreased cell adhesion and compromised cell spreading on various extracellular matrix. Mice embryos lacking Palld exhibit defects in erythropoiesis. Ref.9 Ref.12 Ref.13
Sequence similarities	Belongs to the myotilin/palladin family. Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Sequence caution	The sequence AK031696 differs from that shown. Reason: Frameshift at position 821. The sequence BAB26871.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell junction Cell projection Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Immunoglobulin domain Repeat
Ligand	Actin-binding
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	Z disc Inferred from electronic annotation. Source: UniProtKB-SubCell actin filament Inferred from sequence or structural similarity PubMed 11598191. Source: HGNC focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell lamellipodium Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity PubMed 11598191. Source: HGNC ruffle Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9ET54-1)

Also known as: 200 kDa isoform;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9ET54-2)

The sequence of this isoform differs from the canonical sequence as follows:
     663-887: Missing.
     966-982: Missing.
     1352-1408: TQWHQQPQTTKPKKVRPSASRYAALSDQGLDIKAAFQPEASPSHLTLNSGLVESEDL → ISRH

Isoform 3 (identifier: Q9ET54-3)

Also known as: 90 kDa isoform; 3Ig isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     1-728: Missing.
     966-982: Missing.

Isoform 4 (identifier: Q9ET54-4)

Also known as: 140 kDa isoform; 4Ig isoform;

The sequence of this isoform differs from the canonical sequence as follows:
     1-389: Missing.
     966-982: Missing.

Isoform 5 (identifier: Q9ET54-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-738: Missing.

Note: No experimental confirmation available.

Isoform 6 (identifier: Q9ET54-6)

The sequence of this isoform differs from the canonical sequence as follows:
     966-982: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1408

1408

Palladin

PRO_0000302721

Regions

Domain

278 – 367

90

Ig-like C2-type 1

Domain

448 – 546

99

Ig-like C2-type 2

Domain

1026 – 1110

85

Ig-like C2-type 3

Domain

1160 – 1251

92

Ig-like C2-type 4

Domain

1259 – 1349

91

Ig-like C2-type 5

Region

569 – 573

5

Interaction with VASP

Region

653 – 683

31

Interaction with LASP1

Region

683 – 713

31

Interaction with ARGBP2, SPIN90 and SRC By similarity

Region

782 – 842

61

Interaction with EPS8

Region

807 – 842

36

Interaction with ARGBP2, SPIN90, SRC and PFN1 By similarity

Region

830 – 834

5

Interaction with VASP

Region

1162 – 1251

90

Interaction with EZR By similarity

Region

1261 – 1351

91

Interaction with EZR By similarity

Compositional bias

570 – 574

5

Poly-Pro

Compositional bias

640 – 872

233

Pro-rich

Amino acid modifications

Modified residue

700

1

Phosphoserine By similarity

Modified residue

704

1

Phosphoserine By similarity

Modified residue

901

1

Phosphoserine By similarity

Modified residue

1004

1

Phosphoserine By similarity

Modified residue

1009

1

Phosphoserine Ref.15

Modified residue

1126

1

Phosphoserine By similarity

Modified residue

1129

1

Phosphoserine By similarity

Modified residue

1131

1

Phosphoserine By similarity

Modified residue

1141

1

Phosphoserine Ref.14

Modified residue

1143

1

Phosphoserine; by PKB/AKT1 Ref.14

Modified residue

1146

1

Phosphoserine Ref.14 Ref.16

Disulfide bond

299 ↔ 351

By similarity

Disulfide bond

469 ↔ 528

By similarity

Disulfide bond

1181 ↔ 1233

By similarity

Natural variations

Alternative sequence

1 – 738

738

Missing in isoform 5.

VSP_027931

Alternative sequence

1 – 728

728

Missing in isoform 3.

VSP_027932

Alternative sequence

1 – 389

389

Missing in isoform 4.

VSP_027933

Alternative sequence

663 – 887

225

Missing in isoform 2.

VSP_027934

Alternative sequence

966 – 982

17

Missing in isoform 2, isoform 3, isoform 4 and isoform 6.

VSP_027935

Alternative sequence

1352 – 1408

57

TQWHQ…ESEDL → ISRH in isoform 2.

VSP_027936

Experimental info

Sequence conflict

1022

1

N → K in AK031696. Ref.5

Sequence conflict

1053

1

P → T in AK031696. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

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.

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1408

Helix Strand Turn

Details...

Beta strand

1035 – 1038

4

Beta strand

1043 – 1048

6

Beta strand

1058 – 1061

4

Beta strand

1071 – 1077

7

Beta strand

1081 – 1089

9

Turn

1092 – 1094

3

Beta strand

1096 – 1101

6

Beta strand

1114 – 1118

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (200 kDa isoform) [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 5BCF647C110619C9	FASTA	1,408	152,131
	10 20 30 40 50 60 MSETSSHDSF YDSLSDVQEE GKSADFFPGL SAFLSQEEIN KSLDLARRAI DSSETEDFDS 70 80 90 100 110 120 EKEISQIFSK SPISLCETPS HEEPKSGKQT SSERPQDSRR APVQPLTGDQ AERITSPGSK 130 140 150 160 170 180 RKPGVSPLLA SPSYIRSLRK AEKRGAKNPN PSSKPKTAQQ SKAGPQSQLC DKAASFIEEL 190 200 210 220 230 240 TSIFREAAKP RNRSPNGESS SPDSGYLSPK NQPSALMSAS ASQSPTADQL DQLEMDAEVK 250 260 270 280 290 300 QAQGSLCYQA HQASEETLPL AHIPHPQPQK ARHLPTAPRF IQKLRSQEVA EGSRVYLECR 310 320 330 340 350 360 VTGNPTPRVR WFCEGKELYN SPDVQIHCES GELHTLVIAE AFEDDTGRYT CLATNPSGSD 370 380 390 400 410 420 STSAEVFIEG ASSTDSDSES LSFISKAGAM PQAQKKTTSV SLTIGSSAPK TGVTTAVIQP 430 440 450 460 470 480 LSVPVQQAHS ATSYLCRPDG TTMGCLLPVF TKELQNTAAS EGQVVVLECR VRGAPPLQVQ 490 500 510 520 530 540 WFRQGSEIQD SPDFRILQKK PRSTAEPEEI CTLVIAESFP EDAGIFTCSA TNDYGSVTST 550 560 570 580 590 600 AQLVITSANN ENCSYDSTGE PNSDHFQHFP PPPPILETGS YELASQKPSE IQQVNSPNLG 610 620 630 640 650 660 FSMAALQMQF NTAERETNGV HPSHGVNGLI NGKAYGNKSP PTPTALLSPT KEPPPLLAKP 670 680 690 700 710 720 KLDPLKLQQL QNQVRLEQEA CAWPPAPPGV PCNSSSSGSS APPSPPFPPP PPAFPELAAC 730 740 750 760 770 780 ASPVPSEPMS ALASRATAMQ SSGSFNYARP KQFIAAQNLG PASGLPTPTS SPSSSSLPSP 790 800 810 820 830 840 LSPTPRPFGR APGPPFVEPE AMWGPSSPSP PPPPPPVFSP SAAYPVPDVF PLPPPPPPLP 850 860 870 880 890 900 SSTSHCASPA RFGPSQTPAA FLSALLPSQP PPVAVNALGL PKGVTPAGFP KKSSRTARIA 910 920 930 940 950 960 SDEEIQGTKD AVIQDLERKL RFKEDLLNNG QPRLTYEERM ARRLLGADSA NVFNIQEPEE 970 980 990 1000 1010 1020 TAANQDAGAP RASVGGPLDG QKEYKVSSCE QRLISEIEYR LERSPVDESG DEVQDPDVPV 1030 1040 1050 1060 1070 1080 ENATAPFFEM KLKHYKIFEG MPVTFTCRVA GNPKPKIYWF KDGKQISPKS DHYTIQRDLD 1090 1100 1110 1120 1130 1140 GTCSLHTTAS TLDDDGNYTI MAANPQGRVS CTGRLMVQAV NQRGRSPRSP SGHPHARRPR 1150 1160 1170 1180 1190 1200 SRSRDSGDEN EPIQERFFRP HFLQAPGDLT VQEGKLCRMD CKVSGLPTPD LSWQLDGKPI 1210 1220 1230 1240 1250 1260 RPDSAHKMLV RENGVHSLII EPVTSRDAGI YTCIATNRAG QNSFNLELVV AAKEAHKAPV 1270 1280 1290 1300 1310 1320 FMEKLQNTGV ADGYPVRLEC RVSGVPPPQI FWKKENESLT HSTERVSMHQ DNHGYICLLI 1330 1340 1350 1360 1370 1380 QGATKEDAGW YTVSAKNEAG IVSCTARLDV YTQWHQQPQT TKPKKVRPSA SRYAALSDQG 1390 1400 LDIKAAFQPE ASPSHLTLNS GLVESEDL
	Isoform 2 [UniParc]. Checksum: 2E4249532BDEACEB	FASTA	1,113	122,090
	10 20 30 40 50 60 MSETSSHDSF YDSLSDVQEE GKSADFFPGL SAFLSQEEIN KSLDLARRAI DSSETEDFDS 70 80 90 100 110 120 EKEISQIFSK SPISLCETPS HEEPKSGKQT SSERPQDSRR APVQPLTGDQ AERITSPGSK 130 140 150 160 170 180 RKPGVSPLLA SPSYIRSLRK AEKRGAKNPN PSSKPKTAQQ SKAGPQSQLC DKAASFIEEL 190 200 210 220 230 240 TSIFREAAKP RNRSPNGESS SPDSGYLSPK NQPSALMSAS ASQSPTADQL DQLEMDAEVK 250 260 270 280 290 300 QAQGSLCYQA HQASEETLPL AHIPHPQPQK ARHLPTAPRF IQKLRSQEVA EGSRVYLECR 310 320 330 340 350 360 VTGNPTPRVR WFCEGKELYN SPDVQIHCES GELHTLVIAE AFEDDTGRYT CLATNPSGSD 370 380 390 400 410 420 STSAEVFIEG ASSTDSDSES LSFISKAGAM PQAQKKTTSV SLTIGSSAPK TGVTTAVIQP 430 440 450 460 470 480 LSVPVQQAHS ATSYLCRPDG TTMGCLLPVF TKELQNTAAS EGQVVVLECR VRGAPPLQVQ 490 500 510 520 530 540 WFRQGSEIQD SPDFRILQKK PRSTAEPEEI CTLVIAESFP EDAGIFTCSA TNDYGSVTST 550 560 570 580 590 600 AQLVITSANN ENCSYDSTGE PNSDHFQHFP PPPPILETGS YELASQKPSE IQQVNSPNLG 610 620 630 640 650 660 FSMAALQMQF NTAERETNGV HPSHGVNGLI NGKAYGNKSP PTPTALLSPT KEPPPLLAKP 670 680 690 700 710 720 KLGFPKKSSR TARIASDEEI QGTKDAVIQD LERKLRFKED LLNNGQPRLT YEERMARRLL 730 740 750 760 770 780 GADSANVFNI QEPEETAANQ EYKVSSCEQR LISEIEYRLE RSPVDESGDE VQDPDVPVEN 790 800 810 820 830 840 ATAPFFEMKL KHYKIFEGMP VTFTCRVAGN PKPKIYWFKD GKQISPKSDH YTIQRDLDGT 850 860 870 880 890 900 CSLHTTASTL DDDGNYTIMA ANPQGRVSCT GRLMVQAVNQ RGRSPRSPSG HPHARRPRSR 910 920 930 940 950 960 SRDSGDENEP IQERFFRPHF LQAPGDLTVQ EGKLCRMDCK VSGLPTPDLS WQLDGKPIRP 970 980 990 1000 1010 1020 DSAHKMLVRE NGVHSLIIEP VTSRDAGIYT CIATNRAGQN SFNLELVVAA KEAHKAPVFM 1030 1040 1050 1060 1070 1080 EKLQNTGVAD GYPVRLECRV SGVPPPQIFW KKENESLTHS TERVSMHQDN HGYICLLIQG 1090 1100 1110 ATKEDAGWYT VSAKNEAGIV SCTARLDVYI SRH
	Isoform 3 (90 kDa isoform) (3Ig isoform) [UniParc]. Checksum: 87C70C4EDC263B5E	FASTA	663	72,368
	10 20 30 40 50 60 MSALASRATA MQSSGSFNYA RPKQFIAAQN LGPASGLPTP TSSPSSSSLP SPLSPTPRPF 70 80 90 100 110 120 GRAPGPPFVE PEAMWGPSSP SPPPPPPPVF SPSAAYPVPD VFPLPPPPPP LPSSTSHCAS 130 140 150 160 170 180 PARFGPSQTP AAFLSALLPS QPPPVAVNAL GLPKGVTPAG FPKKSSRTAR IASDEEIQGT 190 200 210 220 230 240 KDAVIQDLER KLRFKEDLLN NGQPRLTYEE RMARRLLGAD SANVFNIQEP EETAANQEYK 250 260 270 280 290 300 VSSCEQRLIS EIEYRLERSP VDESGDEVQD PDVPVENATA PFFEMKLKHY KIFEGMPVTF 310 320 330 340 350 360 TCRVAGNPKP KIYWFKDGKQ ISPKSDHYTI QRDLDGTCSL HTTASTLDDD GNYTIMAANP 370 380 390 400 410 420 QGRVSCTGRL MVQAVNQRGR SPRSPSGHPH ARRPRSRSRD SGDENEPIQE RFFRPHFLQA 430 440 450 460 470 480 PGDLTVQEGK LCRMDCKVSG LPTPDLSWQL DGKPIRPDSA HKMLVRENGV HSLIIEPVTS 490 500 510 520 530 540 RDAGIYTCIA TNRAGQNSFN LELVVAAKEA HKAPVFMEKL QNTGVADGYP VRLECRVSGV 550 560 570 580 590 600 PPPQIFWKKE NESLTHSTER VSMHQDNHGY ICLLIQGATK EDAGWYTVSA KNEAGIVSCT 610 620 630 640 650 660 ARLDVYTQWH QQPQTTKPKK VRPSASRYAA LSDQGLDIKA AFQPEASPSH LTLNSGLVES EDL
	Isoform 4 (140 kDa isoform) (4Ig isoform) [UniParc]. Checksum: 5E630ABBBF42CB74	FASTA	1,002	108,287
	10 20 30 40 50 60 MPQAQKKTTS VSLTIGSSAP KTGVTTAVIQ PLSVPVQQAH SATSYLCRPD GTTMGCLLPV 70 80 90 100 110 120 FTKELQNTAA SEGQVVVLEC RVRGAPPLQV QWFRQGSEIQ DSPDFRILQK KPRSTAEPEE 130 140 150 160 170 180 ICTLVIAESF PEDAGIFTCS ATNDYGSVTS TAQLVITSAN NENCSYDSTG EPNSDHFQHF 190 200 210 220 230 240 PPPPPILETG SYELASQKPS EIQQVNSPNL GFSMAALQMQ FNTAERETNG VHPSHGVNGL 250 260 270 280 290 300 INGKAYGNKS PPTPTALLSP TKEPPPLLAK PKLDPLKLQQ LQNQVRLEQE ACAWPPAPPG 310 320 330 340 350 360 VPCNSSSSGS SAPPSPPFPP PPPAFPELAA CASPVPSEPM SALASRATAM QSSGSFNYAR 370 380 390 400 410 420 PKQFIAAQNL GPASGLPTPT SSPSSSSLPS PLSPTPRPFG RAPGPPFVEP EAMWGPSSPS 430 440 450 460 470 480 PPPPPPPVFS PSAAYPVPDV FPLPPPPPPL PSSTSHCASP ARFGPSQTPA AFLSALLPSQ 490 500 510 520 530 540 PPPVAVNALG LPKGVTPAGF PKKSSRTARI ASDEEIQGTK DAVIQDLERK LRFKEDLLNN 550 560 570 580 590 600 GQPRLTYEER MARRLLGADS ANVFNIQEPE ETAANQEYKV SSCEQRLISE IEYRLERSPV 610 620 630 640 650 660 DESGDEVQDP DVPVENATAP FFEMKLKHYK IFEGMPVTFT CRVAGNPKPK IYWFKDGKQI 670 680 690 700 710 720 SPKSDHYTIQ RDLDGTCSLH TTASTLDDDG NYTIMAANPQ GRVSCTGRLM VQAVNQRGRS 730 740 750 760 770 780 PRSPSGHPHA RRPRSRSRDS GDENEPIQER FFRPHFLQAP GDLTVQEGKL CRMDCKVSGL 790 800 810 820 830 840 PTPDLSWQLD GKPIRPDSAH KMLVRENGVH SLIIEPVTSR DAGIYTCIAT NRAGQNSFNL 850 860 870 880 890 900 ELVVAAKEAH KAPVFMEKLQ NTGVADGYPV RLECRVSGVP PPQIFWKKEN ESLTHSTERV 910 920 930 940 950 960 SMHQDNHGYI CLLIQGATKE DAGWYTVSAK NEAGIVSCTA RLDVYTQWHQ QPQTTKPKKV 970 980 990 1000 RPSASRYAAL SDQGLDIKAA FQPEASPSHL TLNSGLVESE DL
	Isoform 5 [UniParc]. Checksum: CCF8E48D7ADEDDD6	FASTA	670	72,985
	10 20 30 40 50 60 MQSSGSFNYA RPKQFIAAQN LGPASGLPTP TSSPSSSSLP SPLSPTPRPF GRAPGPPFVE 70 80 90 100 110 120 PEAMWGPSSP SPPPPPPPVF SPSAAYPVPD VFPLPPPPPP LPSSTSHCAS PARFGPSQTP 130 140 150 160 170 180 AAFLSALLPS QPPPVAVNAL GLPKGVTPAG FPKKSSRTAR IASDEEIQGT KDAVIQDLER 190 200 210 220 230 240 KLRFKEDLLN NGQPRLTYEE RMARRLLGAD SANVFNIQEP EETAANQDAG APRASVGGPL 250 260 270 280 290 300 DGQKEYKVSS CEQRLISEIE YRLERSPVDE SGDEVQDPDV PVENATAPFF EMKLKHYKIF 310 320 330 340 350 360 EGMPVTFTCR VAGNPKPKIY WFKDGKQISP KSDHYTIQRD LDGTCSLHTT ASTLDDDGNY 370 380 390 400 410 420 TIMAANPQGR VSCTGRLMVQ AVNQRGRSPR SPSGHPHARR PRSRSRDSGD ENEPIQERFF 430 440 450 460 470 480 RPHFLQAPGD LTVQEGKLCR MDCKVSGLPT PDLSWQLDGK PIRPDSAHKM LVRENGVHSL 490 500 510 520 530 540 IIEPVTSRDA GIYTCIATNR AGQNSFNLEL VVAAKEAHKA PVFMEKLQNT GVADGYPVRL 550 560 570 580 590 600 ECRVSGVPPP QIFWKKENES LTHSTERVSM HQDNHGYICL LIQGATKEDA GWYTVSAKNE 610 620 630 640 650 660 AGIVSCTARL DVYTQWHQQP QTTKPKKVRP SASRYAALSD QGLDIKAAFQ PEASPSHLTL 670 NSGLVESEDL
	Isoform 6 [UniParc]. Checksum: FF63541E07E7E676	FASTA	1,391	150,553
	10 20 30 40 50 60 MSETSSHDSF YDSLSDVQEE GKSADFFPGL SAFLSQEEIN KSLDLARRAI DSSETEDFDS 70 80 90 100 110 120 EKEISQIFSK SPISLCETPS HEEPKSGKQT SSERPQDSRR APVQPLTGDQ AERITSPGSK 130 140 150 160 170 180 RKPGVSPLLA SPSYIRSLRK AEKRGAKNPN PSSKPKTAQQ SKAGPQSQLC DKAASFIEEL 190 200 210 220 230 240 TSIFREAAKP RNRSPNGESS SPDSGYLSPK NQPSALMSAS ASQSPTADQL DQLEMDAEVK 250 260 270 280 290 300 QAQGSLCYQA HQASEETLPL AHIPHPQPQK ARHLPTAPRF IQKLRSQEVA EGSRVYLECR 310 320 330 340 350 360 VTGNPTPRVR WFCEGKELYN SPDVQIHCES GELHTLVIAE AFEDDTGRYT CLATNPSGSD 370 380 390 400 410 420 STSAEVFIEG ASSTDSDSES LSFISKAGAM PQAQKKTTSV SLTIGSSAPK TGVTTAVIQP 430 440 450 460 470 480 LSVPVQQAHS ATSYLCRPDG TTMGCLLPVF TKELQNTAAS EGQVVVLECR VRGAPPLQVQ 490 500 510 520 530 540 WFRQGSEIQD SPDFRILQKK PRSTAEPEEI CTLVIAESFP EDAGIFTCSA TNDYGSVTST 550 560 570 580 590 600 AQLVITSANN ENCSYDSTGE PNSDHFQHFP PPPPILETGS YELASQKPSE IQQVNSPNLG 610 620 630 640 650 660 FSMAALQMQF NTAERETNGV HPSHGVNGLI NGKAYGNKSP PTPTALLSPT KEPPPLLAKP 670 680 690 700 710 720 KLDPLKLQQL QNQVRLEQEA CAWPPAPPGV PCNSSSSGSS APPSPPFPPP PPAFPELAAC 730 740 750 760 770 780 ASPVPSEPMS ALASRATAMQ SSGSFNYARP KQFIAAQNLG PASGLPTPTS SPSSSSLPSP 790 800 810 820 830 840 LSPTPRPFGR APGPPFVEPE AMWGPSSPSP PPPPPPVFSP SAAYPVPDVF PLPPPPPPLP 850 860 870 880 890 900 SSTSHCASPA RFGPSQTPAA FLSALLPSQP PPVAVNALGL PKGVTPAGFP KKSSRTARIA 910 920 930 940 950 960 SDEEIQGTKD AVIQDLERKL RFKEDLLNNG QPRLTYEERM ARRLLGADSA NVFNIQEPEE 970 980 990 1000 1010 1020 TAANQEYKVS SCEQRLISEI EYRLERSPVD ESGDEVQDPD VPVENATAPF FEMKLKHYKI 1030 1040 1050 1060 1070 1080 FEGMPVTFTC RVAGNPKPKI YWFKDGKQIS PKSDHYTIQR DLDGTCSLHT TASTLDDDGN 1090 1100 1110 1120 1130 1140 YTIMAANPQG RVSCTGRLMV QAVNQRGRSP RSPSGHPHAR RPRSRSRDSG DENEPIQERF 1150 1160 1170 1180 1190 1200 FRPHFLQAPG DLTVQEGKLC RMDCKVSGLP TPDLSWQLDG KPIRPDSAHK MLVRENGVHS 1210 1220 1230 1240 1250 1260 LIIEPVTSRD AGIYTCIATN RAGQNSFNLE LVVAAKEAHK APVFMEKLQN TGVADGYPVR 1270 1280 1290 1300 1310 1320 LECRVSGVPP PQIFWKKENE SLTHSTERVS MHQDNHGYIC LLIQGATKED AGWYTVSAKN 1330 1340 1350 1360 1370 1380 EAGIVSCTAR LDVYTQWHQQ PQTTKPKKVR PSASRYAALS DQGLDIKAAF QPEASPSHLT 1390 LNSGLVESED L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-1408 (ISOFORM 6). Strain: C57BL/6. Tissue: Eye.
[3]	"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H. DNA Res. 11:127-135(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 311-1408 (ISOFORM 6). Tissue: Embryonic tail.
[4]	"Characterization of palladin, a novel protein localized to stress fibers and cell adhesions." Parast M.M., Otey C.A. J. Cell Biol. 150:643-656(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 900-1408 (ISOFORM 6), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PHOSPHORYLATION. Strain: Swiss Webster / NIH.
[5]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1015-1408 (ISOFORM 1). Strain: C57BL/6J.
[6]	"Palladin is expressed preferentially in excitatory terminals in the rat central nervous system." Hwang S.J., Pagliardini S., Boukhelifa M., Parast M.M., Otey C.A., Rustioni A., Valtschanoff J.G. J. Comp. Neurol. 436:211-224(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[7]	"Palladin is a novel binding partner for Ena/VASP family members." Boukhelifa M., Parast M.M., Bear J.E., Gertler F.B., Otey C.A. Cell Motil. Cytoskeleton 58:17-29(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VASP.
[8]	"The palladin/myotilin/myopalladin family of actin-associated scaffolds." Otey C.A., Rachlin A., Moza M., Arneman D., Carpen O. Int. Rev. Cytol. 246:31-58(2005) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1; 3 AND 4).
[9]	"Disruption of palladin results in neural tube closure defects in mice." Luo H., Liu X., Wang F., Huang Q., Shen S., Wang L., Xu G., Sun X., Kong H., Gu M., Chen S., Chen Z., Wang Z. Mol. Cell. Neurosci. 29:507-515(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[10]	"Identification of palladin isoforms and characterization of an isoform-specific interaction between Lasp-1 and palladin." Rachlin A.S., Otey C.A. J. Cell Sci. 119:995-1004(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ALTERNATIVE SPLICING (ISOFORMS 3 AND 4), INTERACTION OF ISOFORM 3 WITH LASP1.
[11]	"Palladin binds to Eps8 and enhances the formation of dorsal ruffles and podosomes in vascular smooth muscle cells." Goicoechea S., Arneman D., Disanza A., Garcia-Mata R., Scita G., Otey C.A. J. Cell Sci. 119:3316-3324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH EPS8.
[12]	"Disruption of palladin leads to defects in definitive erythropoiesis by interfering erythroblastic island formation in mouse fetal liver." Liu X.-S., Li X.-H., Wang Y., Shu R.-Z., Wang L., Lu S.-Y., Kong H., Jin Y.-E., Zhang L.-J., Fei J., Chen S.-J., Chen Z., Gu M.-M., Lu Z.-Y., Wang Z.-G. Blood 110:870-876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[13]	"Palladin regulates cell and extracellular matrix interaction through maintaining normal actin cytoskeleton architecture and stabilizing beta1-integrin." Liu X.-S., Luo H.-J., Yang H., Wang L., Kong H., Jin Y.-E., Wang F., Gu M.-M., Chen Z., Lu Z.-Y., Wang Z.-G. J. Cell. Biochem. 100:1288-1300(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[14]	"A differential phosphoproteomic analysis of retinoic acid-treated P19 cells." Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D. J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1141; SER-1143 AND SER-1146, MASS SPECTROMETRY. Tissue: Teratocarcinoma.
[15]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1009, MASS SPECTROMETRY. Tissue: Liver.
[16]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1146, MASS SPECTROMETRY. Tissue: Melanoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC121881 Genomic DNA. No translation available.
AC139846 Genomic DNA. No translation available.
AC147616 Genomic DNA. No translation available.
BC076588 mRNA. Translation: AAH76588.1.
BC127081 mRNA. Translation: AAI27082.1.
AK173081 mRNA. Translation: BAD32359.1.
AF205078 mRNA. Translation: AAG00078.1.
AK010350 mRNA. Translation: BAB26871.1. Different initiation.
AK031696 mRNA. No translation available.

IPI

IPI00622946.
IPI00756702.
IPI00856302.
IPI00856926.
IPI00858000.
IPI00858129.

RefSeq

NP_001074859.1. NM_001081390.1.

UniGene

Mm.29933.
Mm.458226.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LQR	NMR	-	A	1022-1126	[»]

ProteinModelPortal

Q9ET54.

SMR

Q9ET54. Positions 270-545, 1022-1393.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-4129416.

PTM databases

PhosphoSite

Q9ET54.

Proteomic databases

PaxDb

Q9ET54.

PRIDE

Q9ET54.

Protocols and materials databases

DNASU

72333.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000034057; ENSMUSP00000034057; ENSMUSG00000058056.
ENSMUST00000121200; ENSMUSP00000112374; ENSMUSG00000058056.
ENSMUST00000121493; ENSMUSP00000113874; ENSMUSG00000058056.
ENSMUST00000121785; ENSMUSP00000112442; ENSMUSG00000058056.

GeneID

72333.

KEGG

mmu:72333.

UCSC

uc009lud.1. mouse.
uc009lue.1. mouse.
uc009luf.1. mouse.
uc009lug.2. mouse.

Organism-specific databases

CTD

23022.

MGI

MGI:1919583. Palld.

Rouge

Search...

Phylogenomic databases

eggNOG

NOG136920.

GeneTree

ENSGT00690000101977.

HOGENOM

HOG000028074.

HOVERGEN

HBG059166.

InParanoid

Q9ET54.

OMA

TRPSYIR.

OrthoDB

EOG434W5H.

Gene expression databases

ArrayExpress

Q9ET54.

Bgee

Q9ET54.

CleanEx

MM_PALLD.

Genevestigator

Q9ET54.

Family and domain databases

Gene3D

2.60.40.10. 6 hits.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
[Graphical view]

Pfam

PF07679. I-set. 5 hits.
[Graphical view]

SMART

SM00408. IGc2. 5 hits.
[Graphical view]

PROSITE

PS50835. IG_LIKE. 5 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

PALLD. mouse.

NextBio

336044.

SOURCE

Search...

Entry information

Entry name

PALLD_MOUSE

Accession

Primary (citable) accession number: Q9ET54
Secondary accession number(s): A0JNZ3 , Q69ZT7, Q6DFX7, Q9CWW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 29, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program