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Q9ET32

- BGAT1_RAT

UniProt

Q9ET32 - BGAT1_RAT

Protein

Histo-blood group ABO system transferase 1

Gene

Abo

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Posseses strong A transferase activity and a weak B transferase activity.1 Publication

    Catalytic activityi

    UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
    UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211UDP-N-acetyl-galactosamineBy similarity
    Metal bindingi206 – 2061ManganeseBy similarity
    Metal bindingi208 – 2081ManganeseBy similarity
    Binding sitei228 – 2281Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei240 – 2401Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei298 – 2981Glycoprotein fucosyl-galactosyl groupBy similarity
    Binding sitei321 – 3211Glycoprotein fucosyl-galactosyl groupBy similarity

    GO - Molecular functioni

    1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
    2. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: RGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to bacterium Source: RGD
    2. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT6. Glycosyltransferase Family 6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histo-blood group ABO system transferase 1
    Alternative name(s):
    Blood group A glycosyltransferase 1
    Cis-AB transferase 1
    Fucosylglycoprotein 3-alpha-galactosyltransferase
    Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
    Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
    Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
    Histo-blood group A transferase
    Short name:
    A transferase
    Histo-blood group B transferase
    Short name:
    B transferase
    N-acetylgalactosaminyltransferase A blood group-like enzyme
    NAGAT 1
    Gene namesi
    Name:Abo
    Synonyms:Abo1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi628609. Abo.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
    Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. Golgi cisterna membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348Histo-blood group ABO system transferase 1PRO_0000356179Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ9ET32.

    Expressioni

    Tissue specificityi

    Tongue, esophagus, large intestine, stomach, caecum, pancreas, uterus, seminal vesicle, submaxillary gland, parotid gland, thyroid gland, parathyroid gland, salivary gland and thymus (at protein level). Esophagus, large intestine, stomach, kidney, urinary bladder, uterus and thymus.2 Publications

    Inductioni

    During a Nippostrongylus brasiliensis parasite infection. The expression is a transient event, with a maximum at day 6 of the 13-day-long infection.1 Publication

    Gene expression databases

    GenevestigatoriQ9ET32.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ET32.
    SMRiQ9ET32. Positions 58-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 348316LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni116 – 1183UDP-N-acetyl-galactosamine bindingBy similarity
    Regioni206 – 2083UDP-N-acetyl-galactosamine bindingBy similarity

    Domaini

    The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

    Sequence similaritiesi

    Belongs to the glycosyltransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43612.
    GeneTreeiENSGT00400000022032.
    HOGENOMiHOG000234339.
    HOVERGENiHBG003563.
    InParanoidiQ9ET32.
    KOiK00709.
    OMAiHNITIAT.
    OrthoDBiEOG7BZVSQ.
    PhylomeDBiQ9ET32.
    TreeFamiTF330991.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10462. PTHR10462. 1 hit.
    PfamiPF03414. Glyco_transf_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9ET32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLRGRPKCY SLHLGILPFI VLVLVFFGYG FLSHKIQEFR NPGGETCMAT    50
    RQTDVQKVVS VPRMAYPQPN VLTPIRNDVL VFTPWLAPII WEGTFNIDIL 100
    NEQFKLQNTT IGLTVFAIKK YVVFLKLFLE TAEQHFMVGH KVIYYVFTDR 150
    PSDVPQVPLG AGRKLVVLTV RNYTRWQDVS MHRMEMISHF SEQRFQHEVD 200
    YLVCGDVDMK FSDHVGVEIL SALFGTLHPG FYRSRRESFT YERRPKSQAY 250
    IPRDEGDFYY AGGFFGGSVV EVHHLTKACH QAMVEDQANG IEAVWHDESH 300
    LNKYLLYHKP TKVLSPEYVW DQKLLGWPSI MKKLRYVAVP KNHQAIRN 348
    Length:348
    Mass (Da):40,375
    Last modified:March 1, 2001 - v1
    Checksum:iFC5E572B100ED7B3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti22 – 221L → I in allele A4.
    Natural varianti24 – 241L → S in allele A3.
    Natural varianti35 – 351K → R in allele A2 and allele A3.
    Natural varianti36 – 361I → F in allele A2 and allele A4.
    Natural varianti37 – 371Q → H in allele A2.
    Natural varianti43 – 442GG → AV in allele A2.
    Natural varianti46 – 461T → S in allele A4.
    Natural varianti53 – 531T → M in allele A2, allele A3 and allele A4.
    Natural varianti56 – 572QK → HE in allele A2, allele A3 and allele A4.
    Natural varianti65 – 651A → T in allele A3 and allele A4.
    Natural varianti70 – 701N → K in allele A3 and allele A4.
    Natural varianti71 – 733VLT → MLK in allele A4.
    Natural varianti75 – 751I → T in allele A2 and allele A3.
    Natural varianti106 – 1061L → T in allele A4.
    Natural varianti107 – 1071Q → R in allele A2 and allele A4.
    Natural varianti146 – 1461V → I in allele A3.
    Natural varianti152 – 1521S → A in allele A2, allele A3 and allele A4.
    Natural varianti164 – 1641K → R in allele A2, allele A3 and allele A4.
    Natural varianti197 – 1971H → Y in allele A2.
    Natural varianti205 – 2051G → A in allele A4.
    Natural varianti222 – 2221A → S in allele A4.
    Natural varianti233 – 2331R → S in allele A4.
    Natural varianti235 – 2351R → Q in allele A4.
    Natural varianti245 – 2451P → S in allele A4.
    Natural varianti253 – 2531R → G in allele A4.
    Natural varianti270 – 2701V → L in allele A2, allele A3 and allele A4.
    Natural varianti281 – 2811Q → K in allele A2, allele A3 and allele A4.
    Natural varianti284 – 2841V → L in allele A4.
    Natural varianti287 – 2871Q → K in allele A2 and allele A3.
    Natural varianti300 – 3001H → Y in allele A2 and allele A3.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264018 mRNA. Translation: AAF74758.2.
    AF296761 Genomic DNA. Translation: AAL98710.1.
    AF296762 mRNA. Translation: AAL98711.1.
    AF469945 mRNA. Translation: AAL82445.1.
    AF469946 mRNA. Translation: AAL82446.1.
    AH011509 Genomic DNA. Translation: AAL82447.1.
    AB081649 mRNA. Translation: BAC16245.1.
    AB081650 mRNA. Translation: BAC16246.1.
    AB081651 mRNA. Translation: BAC16247.1.
    RefSeqiNP_075582.3. NM_023094.3.
    UniGeneiRn.229737.

    Genome annotation databases

    EnsembliENSRNOT00000006553; ENSRNOP00000006553; ENSRNOG00000039906.
    ENSRNOT00000074271; ENSRNOP00000065701; ENSRNOG00000039906.
    GeneIDi65270.
    KEGGirno:65270.
    UCSCiRGD:628609. rat.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF264018 mRNA. Translation: AAF74758.2 .
    AF296761 Genomic DNA. Translation: AAL98710.1 .
    AF296762 mRNA. Translation: AAL98711.1 .
    AF469945 mRNA. Translation: AAL82445.1 .
    AF469946 mRNA. Translation: AAL82446.1 .
    AH011509 Genomic DNA. Translation: AAL82447.1 .
    AB081649 mRNA. Translation: BAC16245.1 .
    AB081650 mRNA. Translation: BAC16246.1 .
    AB081651 mRNA. Translation: BAC16247.1 .
    RefSeqi NP_075582.3. NM_023094.3.
    UniGenei Rn.229737.

    3D structure databases

    ProteinModelPortali Q9ET32.
    SMRi Q9ET32. Positions 58-348.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT6. Glycosyltransferase Family 6.

    Proteomic databases

    PRIDEi Q9ET32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000006553 ; ENSRNOP00000006553 ; ENSRNOG00000039906 .
    ENSRNOT00000074271 ; ENSRNOP00000065701 ; ENSRNOG00000039906 .
    GeneIDi 65270.
    KEGGi rno:65270.
    UCSCi RGD:628609. rat.

    Organism-specific databases

    CTDi 26301.
    RGDi 628609. Abo.

    Phylogenomic databases

    eggNOGi NOG43612.
    GeneTreei ENSGT00400000022032.
    HOGENOMi HOG000234339.
    HOVERGENi HBG003563.
    InParanoidi Q9ET32.
    KOi K00709.
    OMAi HNITIAT.
    OrthoDBi EOG7BZVSQ.
    PhylomeDBi Q9ET32.
    TreeFami TF330991.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 614217.

    Gene expression databases

    Genevestigatori Q9ET32.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005076. Glyco_trans_6.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10462. PTHR10462. 1 hit.
    Pfami PF03414. Glyco_transf_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
      Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
      Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A1), FUNCTION, TISSUE SPECIFICITY.
      Strain: BDIX.
      Tissue: Stomach.
    2. "Blood group A glycosyltransferase occurring as alleles with high sequence difference is transiently induced during a Nippostrongylus brasiliensis parasite infection."
      Olson F.J., Johansson M.E., Klinga-Levan K., Bouhours D., Enerbaeck L., Hansson G.C., Karlsson N.G.
      J. Biol. Chem. 277:15044-15052(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ALLELES A1 AND A2), INDUCTION.
      Strain: Sprague-Dawley.
    3. "Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
      Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
      J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES A2; A3 AND A4), TISSUE SPECIFICITY.
      Strain: Wistar.

    Entry informationi

    Entry nameiBGAT1_RAT
    AccessioniPrimary (citable) accession number: Q9ET32
    Secondary accession number(s): Q8CFC5
    , Q8CFC6, Q8R005, Q8R4Y3, Q8R552
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 16, 2008
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3