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Q9ET32

- BGAT1_RAT

UniProt

Q9ET32 - BGAT1_RAT

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Protein
Histo-blood group ABO system transferase 1
Gene
Abo, Abo1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Posseses strong A transferase activity and a weak B transferase activity.1 Publication

Catalytic activityi

UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose.
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.

Cofactori

Binds 1 manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211UDP-N-acetyl-galactosamine By similarity
Metal bindingi206 – 2061Manganese By similarity
Metal bindingi208 – 2081Manganese By similarity
Binding sitei228 – 2281Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei240 – 2401Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei298 – 2981Glycoprotein fucosyl-galactosyl group By similarity
Binding sitei321 – 3211Glycoprotein fucosyl-galactosyl group By similarity

GO - Molecular functioni

  1. fucosylgalactoside 3-alpha-galactosyltransferase activity Source: UniProtKB-EC
  2. glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Source: RGD
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. defense response to bacterium Source: RGD
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT6. Glycosyltransferase Family 6.

Names & Taxonomyi

Protein namesi
Recommended name:
Histo-blood group ABO system transferase 1
Alternative name(s):
Blood group A glycosyltransferase 1
Cis-AB transferase 1
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40)
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase (EC:2.4.1.37)
Histo-blood group A transferase
Short name:
A transferase
Histo-blood group B transferase
Short name:
B transferase
N-acetylgalactosaminyltransferase A blood group-like enzyme
NAGAT 1
Gene namesi
Name:Abo
Synonyms:Abo1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi628609. Abo.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein. Secreted
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei12 – 3221Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini33 – 348316Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348Histo-blood group ABO system transferase 1
PRO_0000356179Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9ET32.

Expressioni

Tissue specificityi

Tongue, esophagus, large intestine, stomach, caecum, pancreas, uterus, seminal vesicle, submaxillary gland, parotid gland, thyroid gland, parathyroid gland, salivary gland and thymus (at protein level). Esophagus, large intestine, stomach, kidney, urinary bladder, uterus and thymus.2 Publications

Inductioni

During a Nippostrongylus brasiliensis parasite infection. The expression is a transient event, with a maximum at day 6 of the 13-day-long infection.1 Publication

Gene expression databases

GenevestigatoriQ9ET32.

Structurei

3D structure databases

ProteinModelPortaliQ9ET32.
SMRiQ9ET32. Positions 58-348.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni116 – 1183UDP-N-acetyl-galactosamine binding By similarity
Regioni206 – 2083UDP-N-acetyl-galactosamine binding By similarity

Domaini

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG43612.
GeneTreeiENSGT00400000022032.
HOGENOMiHOG000234339.
HOVERGENiHBG003563.
InParanoidiQ9ET32.
KOiK00709.
OMAiHNITIAT.
OrthoDBiEOG7BZVSQ.
PhylomeDBiQ9ET32.
TreeFamiTF330991.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10462. PTHR10462. 1 hit.
PfamiPF03414. Glyco_transf_6. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ET32-1 [UniParc]FASTAAdd to Basket

« Hide

MDLRGRPKCY SLHLGILPFI VLVLVFFGYG FLSHKIQEFR NPGGETCMAT    50
RQTDVQKVVS VPRMAYPQPN VLTPIRNDVL VFTPWLAPII WEGTFNIDIL 100
NEQFKLQNTT IGLTVFAIKK YVVFLKLFLE TAEQHFMVGH KVIYYVFTDR 150
PSDVPQVPLG AGRKLVVLTV RNYTRWQDVS MHRMEMISHF SEQRFQHEVD 200
YLVCGDVDMK FSDHVGVEIL SALFGTLHPG FYRSRRESFT YERRPKSQAY 250
IPRDEGDFYY AGGFFGGSVV EVHHLTKACH QAMVEDQANG IEAVWHDESH 300
LNKYLLYHKP TKVLSPEYVW DQKLLGWPSI MKKLRYVAVP KNHQAIRN 348
Length:348
Mass (Da):40,375
Last modified:March 1, 2001 - v1
Checksum:iFC5E572B100ED7B3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221L → I in allele A4.
Natural varianti24 – 241L → S in allele A3.
Natural varianti35 – 351K → R in allele A2 and allele A3.
Natural varianti36 – 361I → F in allele A2 and allele A4.
Natural varianti37 – 371Q → H in allele A2.
Natural varianti43 – 442GG → AV in allele A2.
Natural varianti46 – 461T → S in allele A4.
Natural varianti53 – 531T → M in allele A2, allele A3 and allele A4.
Natural varianti56 – 572QK → HE in allele A2, allele A3 and allele A4.
Natural varianti65 – 651A → T in allele A3 and allele A4.
Natural varianti70 – 701N → K in allele A3 and allele A4.
Natural varianti71 – 733VLT → MLK in allele A4.
Natural varianti75 – 751I → T in allele A2 and allele A3.
Natural varianti106 – 1061L → T in allele A4.
Natural varianti107 – 1071Q → R in allele A2 and allele A4.
Natural varianti146 – 1461V → I in allele A3.
Natural varianti152 – 1521S → A in allele A2, allele A3 and allele A4.
Natural varianti164 – 1641K → R in allele A2, allele A3 and allele A4.
Natural varianti197 – 1971H → Y in allele A2.
Natural varianti205 – 2051G → A in allele A4.
Natural varianti222 – 2221A → S in allele A4.
Natural varianti233 – 2331R → S in allele A4.
Natural varianti235 – 2351R → Q in allele A4.
Natural varianti245 – 2451P → S in allele A4.
Natural varianti253 – 2531R → G in allele A4.
Natural varianti270 – 2701V → L in allele A2, allele A3 and allele A4.
Natural varianti281 – 2811Q → K in allele A2, allele A3 and allele A4.
Natural varianti284 – 2841V → L in allele A4.
Natural varianti287 – 2871Q → K in allele A2 and allele A3.
Natural varianti300 – 3001H → Y in allele A2 and allele A3.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF264018 mRNA. Translation: AAF74758.2.
AF296761 Genomic DNA. Translation: AAL98710.1.
AF296762 mRNA. Translation: AAL98711.1.
AF469945 mRNA. Translation: AAL82445.1.
AF469946 mRNA. Translation: AAL82446.1.
AH011509 Genomic DNA. Translation: AAL82447.1.
AB081649 mRNA. Translation: BAC16245.1.
AB081650 mRNA. Translation: BAC16246.1.
AB081651 mRNA. Translation: BAC16247.1.
RefSeqiNP_075582.3. NM_023094.3.
UniGeneiRn.229737.

Genome annotation databases

EnsembliENSRNOT00000006553; ENSRNOP00000006553; ENSRNOG00000039906.
ENSRNOT00000074271; ENSRNOP00000065701; ENSRNOG00000039906.
GeneIDi65270.
KEGGirno:65270.
UCSCiRGD:628609. rat.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF264018 mRNA. Translation: AAF74758.2 .
AF296761 Genomic DNA. Translation: AAL98710.1 .
AF296762 mRNA. Translation: AAL98711.1 .
AF469945 mRNA. Translation: AAL82445.1 .
AF469946 mRNA. Translation: AAL82446.1 .
AH011509 Genomic DNA. Translation: AAL82447.1 .
AB081649 mRNA. Translation: BAC16245.1 .
AB081650 mRNA. Translation: BAC16246.1 .
AB081651 mRNA. Translation: BAC16247.1 .
RefSeqi NP_075582.3. NM_023094.3.
UniGenei Rn.229737.

3D structure databases

ProteinModelPortali Q9ET32.
SMRi Q9ET32. Positions 58-348.
ModBasei Search...

Protein family/group databases

CAZyi GT6. Glycosyltransferase Family 6.

Proteomic databases

PRIDEi Q9ET32.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000006553 ; ENSRNOP00000006553 ; ENSRNOG00000039906 .
ENSRNOT00000074271 ; ENSRNOP00000065701 ; ENSRNOG00000039906 .
GeneIDi 65270.
KEGGi rno:65270.
UCSCi RGD:628609. rat.

Organism-specific databases

CTDi 26301.
RGDi 628609. Abo.

Phylogenomic databases

eggNOGi NOG43612.
GeneTreei ENSGT00400000022032.
HOGENOMi HOG000234339.
HOVERGENi HBG003563.
InParanoidi Q9ET32.
KOi K00709.
OMAi HNITIAT.
OrthoDBi EOG7BZVSQ.
PhylomeDBi Q9ET32.
TreeFami TF330991.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 614217.

Gene expression databases

Genevestigatori Q9ET32.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR005076. Glyco_trans_6.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10462. PTHR10462. 1 hit.
Pfami PF03414. Glyco_transf_6. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens."
    Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J.
    Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A1), FUNCTION, TISSUE SPECIFICITY.
    Strain: BDIX.
    Tissue: Stomach.
  2. "Blood group A glycosyltransferase occurring as alleles with high sequence difference is transiently induced during a Nippostrongylus brasiliensis parasite infection."
    Olson F.J., Johansson M.E., Klinga-Levan K., Bouhours D., Enerbaeck L., Hansson G.C., Karlsson N.G.
    J. Biol. Chem. 277:15044-15052(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ALLELES A1 AND A2), INDUCTION.
    Strain: Sprague-Dawley.
  3. "Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase."
    Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E.
    J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES A2; A3 AND A4), TISSUE SPECIFICITY.
    Strain: Wistar.

Entry informationi

Entry nameiBGAT1_RAT
AccessioniPrimary (citable) accession number: Q9ET32
Secondary accession number(s): Q8CFC5
, Q8CFC6, Q8R005, Q8R4Y3, Q8R552
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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