Histo-blood group ABO system transferase 1

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Q9ET32 (BGAT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Histo-blood group ABO system transferase 1

Alternative name(s):
Blood group A glycosyltransferase 1
Cis-AB transferase 1
Fucosylglycoprotein 3-alpha-galactosyltransferase
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase
EC=2.4.1.40
Glycoprotein-fucosylgalactoside alpha-galactosyltransferase
EC=2.4.1.37
Histo-blood group A transferase
Short name=A transferase
Histo-blood group B transferase
Short name=B transferase
N-acetylgalactosaminyltransferase A blood group-like enzyme
NAGAT 1

Gene names

Name:	Abo
Synonyms:	Abo1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Posseses strong A transferase activity and a weak B transferase activity. Ref.1
Catalytic activity	UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose. UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R.
Cofactor	Binds 1 manganese ion per subunit By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid By similarity.
Tissue specificity	Tongue, esophagus, large intestine, stomach, caecum, pancreas, uterus, seminal vesicle, submaxillary gland, parotid gland, thyroid gland, parathyroid gland, salivary gland and thymus (at protein level). Esophagus, large intestine, stomach, kidney, urinary bladder, uterus and thymus. Ref.3
Induction	During a Nippostrongylus brasiliensis parasite infection. The expression is a transient event, with a maximum at day 6 of the 13-day-long infection. Ref.2
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane Secreted
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from expression pattern Ref.2. Source: RGD protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	fucosylgalactoside 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity Inferred from direct assay Ref.2. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 348

348

Histo-blood group ABO system transferase 1

PRO_0000356179

Regions

Topological domain

1 – 11

Cytoplasmic Potential

Transmembrane

12 – 32

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

33 – 348

316

Lumenal Potential

Region

116 – 118

UDP-N-acetyl-galactosamine binding By similarity

Region

206 – 208

UDP-N-acetyl-galactosamine binding By similarity

Sites

Metal binding

206

Manganese By similarity

Metal binding

208

Manganese By similarity

Binding site

121

UDP-N-acetyl-galactosamine By similarity

Binding site

228

Glycoprotein fucosyl-galactosyl group By similarity

Binding site

240

Glycoprotein fucosyl-galactosyl group By similarity

Binding site

298

Glycoprotein fucosyl-galactosyl group By similarity

Binding site

321

Glycoprotein fucosyl-galactosyl group By similarity

Amino acid modifications

Glycosylation

108

N-linked (GlcNAc...) Potential

Natural variations

Natural variant

L → I in allele A4.

Natural variant

L → S in allele A3.

Natural variant

K → R in allele A2 and allele A3.

Natural variant

I → F in allele A2 and allele A4.

Natural variant

Q → H in allele A2.

Natural variant

43 – 44

GG → AV in allele A2.

Natural variant

T → S in allele A4.

Natural variant

T → M in allele A2, allele A3 and allele A4.

Natural variant

56 – 57

QK → HE in allele A2, allele A3 and allele A4.

Natural variant

A → T in allele A3 and allele A4.

Natural variant

N → K in allele A3 and allele A4.

Natural variant

71 – 73

VLT → MLK in allele A4.

Natural variant

I → T in allele A2 and allele A3.

Natural variant

106

L → T in allele A4.

Natural variant

107

Q → R in allele A2 and allele A4.

Natural variant

146

V → I in allele A3.

Natural variant

152

S → A in allele A2, allele A3 and allele A4.

Natural variant

164

K → R in allele A2, allele A3 and allele A4.

Natural variant

197

H → Y in allele A2.

Natural variant

205

G → A in allele A4.

Natural variant

222

A → S in allele A4.

Natural variant

233

R → S in allele A4.

Natural variant

235

R → Q in allele A4.

Natural variant

245

P → S in allele A4.

Natural variant

253

R → G in allele A4.

Natural variant

270

V → L in allele A2, allele A3 and allele A4.

Natural variant

281

Q → K in allele A2, allele A3 and allele A4.

Natural variant

284

V → L in allele A4.

Natural variant

287

Q → K in allele A2 and allele A3.

Natural variant

300

H → Y in allele A2 and allele A3.

Sequences

Sequence

Length

Mass (Da)

Tools

Q9ET32 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: FC5E572B100ED7B3
Show »

FASTA

348

40,375

        10         20         30         40         50         60 
MDLRGRPKCY SLHLGILPFI VLVLVFFGYG FLSHKIQEFR NPGGETCMAT RQTDVQKVVS 

        70         80         90        100        110        120 
VPRMAYPQPN VLTPIRNDVL VFTPWLAPII WEGTFNIDIL NEQFKLQNTT IGLTVFAIKK 

       130        140        150        160        170        180 
YVVFLKLFLE TAEQHFMVGH KVIYYVFTDR PSDVPQVPLG AGRKLVVLTV RNYTRWQDVS 

       190        200        210        220        230        240 
MHRMEMISHF SEQRFQHEVD YLVCGDVDMK FSDHVGVEIL SALFGTLHPG FYRSRRESFT 

       250        260        270        280        290        300 
YERRPKSQAY IPRDEGDFYY AGGFFGGSVV EVHHLTKACH QAMVEDQANG IEAVWHDESH 

       310        320        330        340 
LNKYLLYHKP TKVLSPEYVW DQKLLGWPSI MKKLRYVAVP KNHQAIRN

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References

[1]	"Cloning of a rat gene encoding the histo-blood group A enzyme. Tissue expression of the gene and of the A and B antigens." Cailleau-Thomas A., Le Moullac-Vaidye B., Rocher J., Bouhours D., Szpirer C., Le Pendu J. Eur. J. Biochem. 269:4040-4047(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A1), FUNCTION, TISSUE SPECIFIFICITY. Strain: BDIX. Tissue: Stomach.
[2]	"Blood group A glycosyltransferase occurring as alleles with high sequence difference is transiently induced during a Nippostrongylus brasiliensis parasite infection." Olson F.J., Johansson M.E., Klinga-Levan K., Bouhours D., Enerbaeck L., Hansson G.C., Karlsson N.G. J. Biol. Chem. 277:15044-15052(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ALLELES A1 AND A2), INDUCTION. Strain: Sprague-Dawley.
[3]	"Rat encodes the paralogous gene equivalent of the human histo-blood group ABO gene. Association with antigen expression by overexpression of human ABO transferase." Iwamoto S., Kumada M., Kamesaki T., Okuda H., Kajii E., Inagaki T., Saikawa D., Takeuchi K., Ohgawara S., Takahashi R., Ueda S., Inoue S., Tahara K., Hakamata Y., Kobayashi E. J. Biol. Chem. 277:46463-46469(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES A2; A3 AND A4), TISSUE SPECIFICITY. Strain: Wistar.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF264018 mRNA. Translation: AAF74758.2. AF296761 Genomic DNA. Translation: AAL98710.1. AF296762 mRNA. Translation: AAL98711.1. AF469945 mRNA. Translation: AAL82445.1. AF469946 mRNA. Translation: AAL82446.1. AH011509 Genomic DNA. Translation: AAL82447.1. AB081649 mRNA. Translation: BAC16245.1. AB081650 mRNA. Translation: BAC16246.1. AB081651 mRNA. Translation: BAC16247.1.
IPI	IPI00324292.
RefSeq	NP_075582.3. NM_023094.3.
UniGene	Rn.229737.
3D structure databases
HSSP	HSSP built from PDB template 1LZI based on UniProtKB P16442.
ProteinModelPortal	Q9ET32.
SMR	Q9ET32. Positions 58-348.
ModBase	Search...
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Proteomic databases
PRIDE	Q9ET32.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000006553; ENSRNOP00000006553; ENSRNOG00000039906. ENSRNOT00000074271; ENSRNOP00000065701; ENSRNOG00000039906.
GeneID	65270.
KEGG	rno:65270.
UCSC	RGD:628609. rat.
Organism-specific databases
CTD	65270.
RGD	628609. Abo.
Phylogenomic databases
eggNOG	NOG43612.
GeneTree	ENSGT00400000022032.
HOGENOM	HOG000234339.
HOVERGEN	HBG003563.
InParanoid	Q9ET32.
KO	K00709.
OMA	FCERRFR.
OrthoDB	EOG4D26Q7.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Genevestigator	Q9ET32.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. PTHR10462. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	614217.

Entry information

Entry name

BGAT1_RAT

Accession

Primary (citable) accession number: Q9ET32
Secondary accession number(s): Q8CFC5

Q8R552

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	March 1, 2001
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents