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Protein

E3 ubiquitin-protein ligase RNF114

Gene

Rnf114

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 69RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri92 – 111C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Transferase
Biological processDifferentiation, Spermatogenesis, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF114 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 114
RING-type E3 ubiquitin transferase RNF114Curated
Zinc finger protein 228
Zinc finger protein 313
Gene namesi
Name:Rnf114
Synonyms:Zfp228, Zfp313, Znf228, Znf313
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1933159 Rnf114

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000563081 – 229E3 ubiquitin-protein ligase RNF114Add BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103N6-acetyllysineBy similarity1
Modified residuei113N6-acetyllysineBy similarity1

Post-translational modificationi

Autoubiquitinated. Polyubiquitinated in the presence of E2 enzymes UBE2D1, UBE2D2 and UBE2D3, but only monoubiquitinated in the presence of UBE2E1.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ9ET26
MaxQBiQ9ET26
PaxDbiQ9ET26
PeptideAtlasiQ9ET26
PRIDEiQ9ET26

2D gel databases

REPRODUCTION-2DPAGEiQ9ET26

PTM databases

iPTMnetiQ9ET26
PhosphoSitePlusiQ9ET26

Expressioni

Gene expression databases

BgeeiENSMUSG00000006418
ExpressionAtlasiQ9ET26 baseline and differential
GenevisibleiQ9ET26 MM

Interactioni

Subunit structurei

Interacts with XAF1, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219874, 1 interactor
STRINGi10090.ENSMUSP00000077197

Structurei

3D structure databases

ProteinModelPortaliQ9ET26
SMRiQ9ET26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri30 – 69RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri92 – 111C2HC RNF-typePROSITE-ProRule annotationAdd BLAST20

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEG7 Eukaryota
ENOG4111JWC LUCA
GeneTreeiENSGT00530000063064
HOGENOMiHOG000230946
HOVERGENiHBG074331
InParanoidiQ9ET26
KOiK15697
OMAiIEHIQRR
OrthoDBiEOG091G0M3K
PhylomeDBiQ9ET26
TreeFamiTF331012

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR008598 Di19_Zn_binding_dom
IPR034734 ZF_C2HC_RNF
IPR027370 Znf-RING_LisH
IPR013087 Znf_C2H2_type
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF05605 zf-Di19, 1 hit
PF13445 zf-RING_UBOX, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00355 ZnF_C2H2, 2 hits
PROSITEiView protein in PROSITE
PS51803 ZF_C2HC_RNF, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ET26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAQPESRD GAAQSAKPAS ETDPLSRFTC PVCLEVFEKP VQVPCGHVFC
60 70 80 90 100
SACLQECLKP KKPVCGVCRS ALAPGVRAVE LERQIESIET SCHGCRKNFI
110 120 130 140 150
LSKIRAHVTS CSKYQNYIME GVKATTKDAS LQPRNIPNRY TFPCPYCPEK
160 170 180 190 200
NFDQEGLVEH CKLTHSTDTK SVVCPICASM PWGDPSYRSA NFMEHIQRRH
210 220
RFSYDTFVDY DVDEDDMINQ VLQRSIIDQ
Length:229
Mass (Da):25,745
Last modified:July 3, 2003 - v2
Checksum:i7E500B9687EA0DD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282919 mRNA Translation: AAG01141.2
AF502145 mRNA Translation: AAM22210.1
AK088441 mRNA Translation: BAC40355.1
AK139030 mRNA Translation: BAE23870.1
AK148290 mRNA Translation: BAE28461.1
AK148392 mRNA Translation: BAE28527.1
AK150086 mRNA Translation: BAE29295.1
AK150263 mRNA Translation: BAE29420.1
AK152687 mRNA Translation: BAE31418.1
AK153452 mRNA Translation: BAE32006.1
AK159818 mRNA Translation: BAE35398.1
BC054416 mRNA Translation: AAH54416.1
BC085146 mRNA Translation: AAH85146.1
CCDSiCCDS17101.1
RefSeqiNP_109668.2, NM_030743.5
XP_006500502.1, XM_006500439.1
UniGeneiMm.22225

Genome annotation databases

EnsembliENSMUST00000078050; ENSMUSP00000077197; ENSMUSG00000006418
ENSMUST00000109214; ENSMUSP00000104837; ENSMUSG00000006418
GeneIDi81018
KEGGimmu:81018
UCSCiuc008nzv.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRN114_MOUSE
AccessioniPrimary (citable) accession number: Q9ET26
Secondary accession number(s): Q3UFU8, Q8K5A2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: July 3, 2003
Last modified: June 20, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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