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Protein

Glycogen phosphorylase, liver form

Gene

Pygl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity).By similarity

Catalytic activityi

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate.

Cofactori

Enzyme regulationi

Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose-6-phosphate allosterically inhibit, phosphorylase B (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76AMPBy similarity1
Sitei109Involved in the association of subunitsBy similarity1
Sitei143Involved in the association of subunitsBy similarity1
Sitei156May be involved in allosteric controlBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: Ensembl
  • glucose homeostasis Source: MGI
  • glycogen catabolic process Source: GO_Central
  • glycogen metabolic process Source: MGI
  • necroptotic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

Nucleotide-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-70221. Glycogen breakdown (glycogenolysis).

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen phosphorylase, liver form (EC:2.4.1.1)
Gene namesi
Name:Pygl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:97829. Pygl.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3008.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001885252 – 850Glycogen phosphorylase, liver formAdd BLAST849

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei15Phosphoserine; by PHK; in form phosphorylase ABy similarity1
Modified residuei204PhosphotyrosineBy similarity1
Modified residuei227PhosphotyrosineBy similarity1
Modified residuei364N6-succinyllysineCombined sources1
Modified residuei430PhosphoserineBy similarity1
Modified residuei524PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei561PhosphoserineBy similarity1
Modified residuei639PhosphoserineBy similarity1
Modified residuei681N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ET01.
MaxQBiQ9ET01.
PaxDbiQ9ET01.
PeptideAtlasiQ9ET01.
PRIDEiQ9ET01.

PTM databases

iPTMnetiQ9ET01.
PhosphoSitePlusiQ9ET01.
SwissPalmiQ9ET01.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021069.
CleanExiMM_PYGL.
ExpressionAtlasiQ9ET01. baseline and differential.
GenevisibleiQ9ET01. MM.

Interactioni

Subunit structurei

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Interacts with PPP1R3B (By similarity).By similarity

Protein-protein interaction databases

BioGridi225292. 1 interactor.
IntActiQ9ET01. 5 interactors.
MINTiMINT-1870110.
STRINGi10090.ENSMUSP00000071231.

Chemistry databases

BindingDBiQ9ET01.

Structurei

3D structure databases

ProteinModelPortaliQ9ET01.
SMRiQ9ET01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycogen phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
GeneTreeiENSGT00390000016886.
HOGENOMiHOG000278444.
HOVERGENiHBG006848.
InParanoidiQ9ET01.
KOiK00688.
OMAiKRAWAEF.
OrthoDBiEOG091G03RB.
TreeFamiTF300309.

Family and domain databases

CDDicd04300. GT1_Glycogen_Phosphorylase. 1 hit.
InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ET01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPLTDQEK RRQISIRGIV GVENVAELKK GFNRHLHFTL VKDRNVATPR
60 70 80 90 100
DYYFALAHTV RDHLVGRWIR TQQHYYDKCP KRVYYLSLEF YMGRTLQNTM
110 120 130 140 150
INLGLQNACD EAIYQLGLDM EELEEIEEDA GLGNGGLGRL AACFLDSMAT
160 170 180 190 200
LGLAAYGYGI RYEYGIFNQK IREGWQVEEA DDWLRHGNPW EKARPEFMLP
210 220 230 240 250
VHFYGRVEHT QTGTKWVDTQ VVLALPYDTP VPGYMNNTVN TMRLWSARAP
260 270 280 290 300
NDFNLQDFNV GDYIQAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
310 320 330 340 350
VAATLQDVIR RFKASKFGSK DGMGTVFDAF PDQVAIQLND THPALAIPEL
360 370 380 390 400
MRIFVDIEKL PWAKAWEITK KTFAYTNHTV LPEALERWPV ELVEKLLPRH
410 420 430 440 450
LEIIYEINQK HLDRIVALFP KDISRMRRMS LIEEEGGKRI NMAHLCIVGC
460 470 480 490 500
HAVNGVAKIH SDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL
510 520 530 540 550
ADLIAEKIGE DYVKDLSQLT KLHSFVSDDI FLREIAKVKQ ENKLKFSQFL
560 570 580 590 600
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVITMYNR IKKDPKKFFV
610 620 630 640 650
PRTVIIGGKA APGYHMAKMI IKLITSVAEV VNNDPMVGSK LKVIFLENYR
660 670 680 690 700
VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
710 720 730 740 750
AEEAGEENLF IFGMRVDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF
760 770 780 790 800
FSPNQPDLFK DIINMLFYHD RFKVFADYEA YVKCQEKVSQ LYMNQKAWNT
810 820 830 840 850
MVLKNIAASG KFSSDRTIKE YAKDIWNMEP SDLKISLSNE SSNGVSANGK
Length:850
Mass (Da):97,463
Last modified:July 27, 2011 - v4
Checksum:iC4BFF6A9AA4E181E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198M → V in AAG00588 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288783 mRNA. Translation: AAG00588.1.
AK140321 mRNA. Translation: BAE24332.1.
AK145989 mRNA. Translation: BAE26811.1.
CCDSiCCDS25957.1.
RefSeqiNP_573461.2. NM_133198.2.
UniGeneiMm.256926.
Mm.447796.

Genome annotation databases

EnsembliENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069.
GeneIDi110095.
KEGGimmu:110095.
UCSCiuc007ntm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF288783 mRNA. Translation: AAG00588.1.
AK140321 mRNA. Translation: BAE24332.1.
AK145989 mRNA. Translation: BAE26811.1.
CCDSiCCDS25957.1.
RefSeqiNP_573461.2. NM_133198.2.
UniGeneiMm.256926.
Mm.447796.

3D structure databases

ProteinModelPortaliQ9ET01.
SMRiQ9ET01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi225292. 1 interactor.
IntActiQ9ET01. 5 interactors.
MINTiMINT-1870110.
STRINGi10090.ENSMUSP00000071231.

Chemistry databases

BindingDBiQ9ET01.
ChEMBLiCHEMBL3008.

Protein family/group databases

CAZyiGT35. Glycosyltransferase Family 35.

PTM databases

iPTMnetiQ9ET01.
PhosphoSitePlusiQ9ET01.
SwissPalmiQ9ET01.

Proteomic databases

EPDiQ9ET01.
MaxQBiQ9ET01.
PaxDbiQ9ET01.
PeptideAtlasiQ9ET01.
PRIDEiQ9ET01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071250; ENSMUSP00000071231; ENSMUSG00000021069.
GeneIDi110095.
KEGGimmu:110095.
UCSCiuc007ntm.2. mouse.

Organism-specific databases

CTDi5836.
MGIiMGI:97829. Pygl.

Phylogenomic databases

eggNOGiKOG2099. Eukaryota.
COG0058. LUCA.
GeneTreeiENSGT00390000016886.
HOGENOMiHOG000278444.
HOVERGENiHBG006848.
InParanoidiQ9ET01.
KOiK00688.
OMAiKRAWAEF.
OrthoDBiEOG091G03RB.
TreeFamiTF300309.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-70221. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

ChiTaRSiPygl. mouse.
PROiQ9ET01.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021069.
CleanExiMM_PYGL.
ExpressionAtlasiQ9ET01. baseline and differential.
GenevisibleiQ9ET01. MM.

Family and domain databases

CDDicd04300. GT1_Glycogen_Phosphorylase. 1 hit.
InterProiIPR011833. Glycg_phsphrylas.
IPR000811. Glyco_trans_35.
[Graphical view]
PANTHERiPTHR11468. PTHR11468. 1 hit.
PfamiPF00343. Phosphorylase. 1 hit.
[Graphical view]
PIRSFiPIRSF000460. Pprylas_GlgP. 1 hit.
TIGRFAMsiTIGR02093. P_ylase. 1 hit.
PROSITEiPS00102. PHOSPHORYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYGL_MOUSE
AccessioniPrimary (citable) accession number: Q9ET01
Secondary accession number(s): Q3UKJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 142 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.