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Q9ESZ8 (GTF2I_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General transcription factor II-I

Short name=GTFII-I
Short name=TFII-I
Alternative name(s):
Bruton tyrosine kinase-associated protein 135
Short name=BAP-135
Short name=BTK-associated protein 135
Gene names
Name:Gtf2i
Synonyms:Bap135, Diws1t
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length998 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box By similarity. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. Ref.7

Subunit structure

Homodimer Potential. Interacts with SRF and PHOX1. Binds a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1). Associates with the PH domain of Bruton's tyrosine kinase (BTK) By similarity. May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with BTK and ARID3A. Interacts with isoform betaof PRKG1 By similarity. Ref.7

Subcellular location

Cytoplasm. Nucleus. Note: Colocalizes with BTK in the cytoplasm By similarity.

Tissue specificity

Ubiquitous.

Post-translational modification

Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation By similarity.

Sumoylated By similarity.

Sequence similarities

Belongs to the TFII-I family.

Contains 6 GTF2I-like repeats.

Sequence caution

The sequence BAB28803.2 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9ESZ8-1)

Also known as: Gamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9ESZ8-2)

Also known as: Beta; Long;

The sequence of this isoform differs from the canonical sequence as follows:
     255-273: Missing.
Isoform 3 (identifier: Q9ESZ8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     255-292: Missing.
Isoform 4 (identifier: Q9ESZ8-4)

Also known as: Delta; Short;

The sequence of this isoform differs from the canonical sequence as follows:
     255-273: Missing.
     293-313: Missing.
Isoform 5 (identifier: Q9ESZ8-5)

The sequence of this isoform differs from the canonical sequence as follows:
     255-313: Missing.
Isoform 6 (identifier: Q9ESZ8-6)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     293-313: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 998997General transcription factor II-I
PRO_0000083873

Regions

Repeat103 – 19795GTF2I-like 1
Repeat352 – 44695GTF2I-like 2
Repeat457 – 55195GTF2I-like 3
Repeat562 – 65695GTF2I-like 4
Repeat724 – 81895GTF2I-like 5
Repeat859 – 95395GTF2I-like 6
Motif319 – 3268Nuclear localization signal Potential
Compositional bias329 – 3379Poly-Pro

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1031Phosphoserine By similarity
Modified residue1301N6-acetyllysine Ref.8
Modified residue2071Phosphoserine By similarity
Modified residue2101Phosphoserine By similarity
Modified residue2481Phosphotyrosine; by BTK By similarity
Modified residue3171Phosphotyrosine; by BTK By similarity
Modified residue3531N6-acetyllysine Ref.8
Modified residue3981Phosphotyrosine; by BTK By similarity
Modified residue4121Phosphoserine; by PKG/PRKG1 By similarity
Modified residue4501N6-acetyllysine Ref.8
Modified residue5031Phosphotyrosine; by BTK By similarity
Modified residue5581Phosphothreonine By similarity
Modified residue6681Phosphoserine By similarity
Modified residue7151N6-acetyllysine Ref.8
Modified residue7841Phosphoserine; by PKG/PRKG1 By similarity
Modified residue8231Phosphoserine By similarity

Natural variations

Alternative sequence255 – 31359Missing in isoform 5.
VSP_003871
Alternative sequence255 – 29238Missing in isoform 3.
VSP_003870
Alternative sequence255 – 27319Missing in isoform 2 and isoform 4.
VSP_003869
Alternative sequence293 – 31321Missing in isoform 4 and isoform 6.
VSP_003872

Experimental info

Sequence conflict51V → A in AAC53569. Ref.1
Sequence conflict621G → R in AAC53569. Ref.1
Sequence conflict127 – 1304ALGK → NTAL in BAB24743. Ref.5
Sequence conflict2541Q → QA Ref.1
Sequence conflict2611G → D Ref.1
Sequence conflict2661L → Q Ref.1
Sequence conflict271 – 2722AL → PM Ref.1
Sequence conflict3141D → G Ref.6
Sequence conflict3341Missing in AAC53569. Ref.1
Sequence conflict5381I → T in AAC53569. Ref.1
Sequence conflict6071L → C in AAC53569. Ref.1
Sequence conflict6211P → L in AAC53569. Ref.1
Sequence conflict6911P → L in AAC02990. Ref.2
Sequence conflict6911P → L in AAC02991. Ref.2
Sequence conflict7481A → T in AAC53569. Ref.1
Sequence conflict8261R → IFLSG in BAB28803. Ref.5
Sequence conflict9661E → Q in BAB28803. Ref.5

Secondary structure

................... 998
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Gamma) [UniParc].

Last modified December 15, 2003. Version 3.
Checksum: 3BC228A2F4F880CF

FASTA998112,265
        10         20         30         40         50         60 
MAQVVMSALP AEDEESSESR MVVTFLMSAL ESMCKELAKS KAEVACIAVY ETDVFVVGTE 

        70         80         90        100        110        120 
RGRAFVNTRK DFQKDFVKYC VEEEEKAAEM HKMKSTTQAN RMSVDAVEIE TLRKTVEDYF 

       130        140        150        160        170        180 
CFCYGKALGK STVVPVPYEK MLRDQSAVVV QGLPEGVAFK HPEHYDLATL KWILENKAGI 

       190        200        210        220        230        240 
SFIIKRPFLE PKKHLGGRVL AAEAERSMLS PSGSCGPIKV KTEPTEDSGI SLEMAAVTVK 

       250        260        270        280        290        300 
EESEDPDYYQ YNIQGPSETD GVDEKLPLSK ALQGSHHSSE GNEGTEVEVP AEDSTQHVPS 

       310        320        330        340        350        360 
ETSEDPEVEV TIEDDDYSPP TKRLKSTEPP PPPPVPEPAN AGKRKVREFN FEKWNARITD 

       370        380        390        400        410        420 
LRKQVEELFE RKYAQAIKAK GPVTIPYPLF QSHVEDLYVE GLPEGIPFRR PSTYGIPRLE 

       430        440        450        460        470        480 
RILLAKERIR FVIKKHELLN STREDLQLDK PASGVKEEWY ARITKLRKMV DQLFCKKFAE 

       490        500        510        520        530        540 
ALGSTEAKAV PYQKFEAHPN DLYVEGLPEN IPFRSPSWYG IPRLEKIIQV GNRIKFVIKR 

       550        560        570        580        590        600 
PELLTHSTTE VTQPRTNTPV KEDWNVRITK LRKQVEEIFN LKFAQALGLT EAVKVPYPVF 

       610        620        630        640        650        660 
ESNPEFLYVE GLPEGIPFRS PTWFGIPRLE RIVRGSNKIK FVVKKPELVV SYLPPGMASK 

       670        680        690        700        710        720 
INTKALQSPK RPRSPGSNSK VPEIEVTVEG PNNSSPQTSA VRTPTQTNGS NVPFKPRGRE 

       730        740        750        760        770        780 
FSFEAWNAKI TDLKQKVENL FNEKCGEALG LKQAVKVPFA LFESFPEDFY VEGLPEGVPF 

       790        800        810        820        830        840 
RRPSTFGIPR LEKILRNKAK IKFIIKKPEM FETAIKESTS SKSPPRKINS SPNVNTTASG 

       850        860        870        880        890        900 
VEDLNIIQVT IPDDDNERLS KVEKARQLRE QVNDLFSRKF GEAIGMGFPV KVPYRKITIN 

       910        920        930        940        950        960 
PGCVVVDGMP PGVSFKAPSY LEISSMRRIL DSAEFIKFTV IRPFPGLVIN NQLVDQNESE 

       970        980        990 
GPVIQESAEA SQLEVPVTEE IKETDGSSQI KQEPDPTW 

« Hide

Isoform 2 (Beta) (Long) [UniParc].

Checksum: E001EB13BE19A81F
Show »

FASTA979110,299
Isoform 3 [UniParc].

Checksum: 80E39FB7F85C4A93
Show »

FASTA960108,365
Isoform 4 (Delta) (Short) [UniParc].

Checksum: BC47CBD9FC28F4BD
Show »

FASTA958107,989
Isoform 5 [UniParc].

Checksum: DC04C876B09F26FA
Show »

FASTA939106,055
Isoform 6 (Alpha) [UniParc].

Checksum: 013875885B7085A1
Show »

FASTA977109,955

References

« Hide 'large scale' references
[1]"A mouse single-copy gene, Gtf2i, the homolog of human GTF2I, that is duplicated in the Williams-Beuren syndrome deletion region."
Wang Y.-K., Perez-Jurado L.A., Francke U.
Genomics 48:163-170(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
Tissue: Brain.
[2]"Cloning and expression of two forms of mouse TFII-I."
Johansson E., Hjortsberg K., Roy A.L., Thelander L.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 4).
Strain: C57BL/Kaplan.
Tissue: T-cell lymphoma.
[3]"Genomic organization of the genes Gtf2ird1, Gtf2i, and Ncf1 at the mouse chromosome 5 region syntenic to the human chromosome 7q11.23 Williams syndrome critical region."
Bayarsaihan D., Dunai J., Greally J.M., Kawasaki K., Sumiyama K., Enkhmandakh B., Shimizu N., Ruddle F.H.
Genomics 79:137-143(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 4 AND 6).
Strain: 129/SvJ.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-130 AND 719-998.
Strain: C57BL/6J.
Tissue: Embryo and Testis.
[6]Green E.D.
Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-314 (ISOFORM 2).
Strain: 129/Sv.
[7]"Induction of immunoglobulin heavy-chain transcription through the transcription factor Bright requires TFII-I."
Rajaiya J., Nixon J.C., Ayers N., Desgranges Z.P., Roy A.L., Webb C.F.
Mol. Cell. Biol. 26:4758-4768(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARID3A AND BTK, FUNCTION.
[8]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-130; LYS-353; LYS-450 AND LYS-715, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[9]"Solution structure of the general transcription factor 2I domain in mouse TFII-I protein."
Doi-Katayama Y., Hayashi F., Inoue M., Yabuki T., Aoki M., Seki E., Matsuda T., Kigawa T., Yoshida M., Shirouzu M., Terada T., Hayashizaki Y., Yokoyama S., Hirota H.
Protein Sci. 16:1788-1792(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 733-818.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017085 mRNA. Translation: AAC53569.1.
AF043219 mRNA. Translation: AAC02990.1.
AF043220 mRNA. Translation: AAC02991.1.
AF325177 Genomic DNA. No translation available.
AY030290 mRNA. Translation: AAK49785.1.
AY030291 mRNA. Translation: AAK49786.1.
AY030292 mRNA. Translation: AAK49787.1.
AY030293 mRNA. Translation: AAK49788.1.
BC053044 mRNA. Translation: AAH53044.1.
AK006796 mRNA. Translation: BAB24743.1.
AK013348 mRNA. Translation: BAB28803.2. Different initiation.
AF289666 Genomic DNA. Translation: AAF99338.1.
CCDSCCDS39299.1. [Q9ESZ8-2]
CCDS39300.1. [Q9ESZ8-6]
CCDS39301.1. [Q9ESZ8-1]
CCDS57386.1. [Q9ESZ8-4]
PIRT03763.
RefSeqNP_001074215.1. NM_001080746.2. [Q9ESZ8-1]
NP_001074216.1. NM_001080747.2. [Q9ESZ8-6]
NP_001074217.1. NM_001080748.2. [Q9ESZ8-4]
NP_034495.2. NM_010365.4. [Q9ESZ8-2]
UniGeneMm.261570.
Mm.412191.
Mm.466495.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q60NMR-A733-818[»]
ProteinModelPortalQ9ESZ8.
SMRQ9ESZ8. Positions 104-197, 359-454, 466-556, 571-649, 731-823, 854-954.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200113. 3 interactions.
IntActQ9ESZ8. 5 interactions.
MINTMINT-4097022.

PTM databases

PhosphoSiteQ9ESZ8.

Proteomic databases

MaxQBQ9ESZ8.
PaxDbQ9ESZ8.
PRIDEQ9ESZ8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000059042; ENSMUSP00000049625; ENSMUSG00000060261. [Q9ESZ8-1]
ENSMUST00000082057; ENSMUSP00000080714; ENSMUSG00000060261. [Q9ESZ8-6]
ENSMUST00000111261; ENSMUSP00000106892; ENSMUSG00000060261. [Q9ESZ8-2]
ENSMUST00000173888; ENSMUSP00000133969; ENSMUSG00000060261. [Q9ESZ8-5]
ENSMUST00000174155; ENSMUSP00000133566; ENSMUSG00000060261. [Q9ESZ8-1]
ENSMUST00000174354; ENSMUSP00000134440; ENSMUSG00000060261. [Q9ESZ8-2]
ENSMUST00000174513; ENSMUSP00000133489; ENSMUSG00000060261. [Q9ESZ8-4]
ENSMUST00000174772; ENSMUSP00000133740; ENSMUSG00000060261. [Q9ESZ8-6]
GeneID14886.
KEGGmmu:14886.
UCSCuc008zvj.2. mouse. [Q9ESZ8-1]
uc008zvk.2. mouse. [Q9ESZ8-2]
uc008zvl.2. mouse. [Q9ESZ8-6]
uc008zvm.2. mouse. [Q9ESZ8-4]

Organism-specific databases

CTD2969.
MGIMGI:1202722. Gtf2i.

Phylogenomic databases

eggNOGNOG29608.
GeneTreeENSGT00530000063863.
HOVERGENHBG051856.
InParanoidQ9ESZ8.
KOK03121.
OMAMPPGVAF.
OrthoDBEOG7RRF67.
PhylomeDBQ9ESZ8.
TreeFamTF352524.

Gene expression databases

ArrayExpressQ9ESZ8.
BgeeQ9ESZ8.
CleanExMM_GTF2I.
GenevestigatorQ9ESZ8.

Family and domain databases

Gene3D3.90.1460.10. 6 hits.
InterProIPR004212. GTF2I.
IPR016659. TF_II-I.
[Graphical view]
PfamPF02946. GTF2I. 6 hits.
[Graphical view]
PIRSFPIRSF016441. TF_II-I. 1 hit.
SUPFAMSSF117773. SSF117773. 6 hits.
PROSITEPS51139. GTF2I. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGTF2I. mouse.
EvolutionaryTraceQ9ESZ8.
NextBio287171.
PROQ9ESZ8.
SOURCESearch...

Entry information

Entry nameGTF2I_MOUSE
AccessionPrimary (citable) accession number: Q9ESZ8
Secondary accession number(s): O54700 expand/collapse secondary AC list , O55030, O55031, Q8VHD1, Q8VHD2, Q8VHD3, Q8VHD4, Q9CSB5, Q9D9K9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: December 15, 2003
Last modified: July 9, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot