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Protein

Acylglycerol kinase, mitochondrial

Gene

Agk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively (PubMed:15252046). Does not phosphorylate sphingosine (PubMed:15252046). Independently of its lipid kinase activity, acts as a component of the TIM22 complex (By similarity). The TIM22 complex mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane by forming a twin-pore translocase that uses the membrane potential as the external driving force (By similarity). In the TIM22 complex, required for the import of a subset of metabolite carriers into mitochtondria, such as ANT1/SLC25A4 and SLC25A24, while it is not required for the import of TIMM23 (By similarity). Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth (By similarity).By similarity1 Publication

Caution

Was originally (PubMed:15252046) thought to have ceramide kinase activity. Such activity is however unlikely in vivo.1 Publication

Catalytic activityi

ATP + acylglycerol = ADP + acyl-sn-glycerol 3-phosphate.By similarity1 Publication
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Cofactori

Mg2+By similarity

Kineticsi

  1. KM=45 µM for diacylglycerol1 Publication
  1. Vmax=159 nmol/min/mg enzyme with dioeoylglycerol as substrate1 Publication

Pathwayi: glycerolipid metabolism

This protein is involved in the pathway glycerolipid metabolism, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway glycerolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

  • acylglycerol kinase activity Source: UniProtKB-EC
  • ATP binding Source: UniProtKB-KW
  • ceramide kinase activity Source: MGI
  • diacylglycerol kinase activity Source: UniProtKB-EC
  • lipid kinase activity Source: MGI
  • NAD+ kinase activity Source: InterPro

GO - Biological processi

  • ceramide biosynthetic process Source: MGI
  • glycerolipid metabolic process Source: UniProtKB-UniPathway
  • lipid phosphorylation Source: MGI
  • protein import into mitochondrial inner membrane Source: UniProtKB

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1483206 Glycerophospholipid biosynthesis
SABIO-RKiQ9ESW4
UniPathwayiUPA00230

Chemistry databases

SwissLipidsiSLP:000000885

Names & Taxonomyi

Protein namesi
Recommended name:
Acylglycerol kinase, mitochondrialBy similarity (EC:2.7.1.1071 Publication, EC:2.7.1.941 Publication)
Alternative name(s):
Multiple substrate lipid kinase1 Publication
Short name:
MuLK1 Publication
Short name:
Multi-substrate lipid kinase1 Publication
Gene namesi
Name:AgkImported
Synonyms:Mulk1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1917173 Agk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002523811 – 421Acylglycerol kinase, mitochondrialAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9ESW4
PaxDbiQ9ESW4
PeptideAtlasiQ9ESW4
PRIDEiQ9ESW4

PTM databases

iPTMnetiQ9ESW4
PhosphoSitePlusiQ9ESW4

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000029916
CleanExiMM_AGK
ExpressionAtlasiQ9ESW4 baseline and differential
GenevisibleiQ9ESW4 MM

Interactioni

Subunit structurei

Component of the TIM22 complex, which core is composed of TIMM22, associated with TIMM10 (TIMM10A and/or TIMM10B), TIMM9, AGK and TIMM29.By similarity

Protein-protein interaction databases

BioGridi213759, 1 interactor
IntActiQ9ESW4, 2 interactors
MINTiQ9ESW4
STRINGi10090.ENSMUSP00000031977

Structurei

3D structure databases

ProteinModelPortaliQ9ESW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini58 – 199DAGKcPROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 31HydrophobicBy similarityAdd BLAST17

Sequence similaritiesi

Belongs to the AGK family.Curated

Phylogenomic databases

eggNOGiKOG4435 Eukaryota
COG1597 LUCA
GeneTreeiENSGT00690000101761
HOGENOMiHOG000252977
HOVERGENiHBG080751
InParanoidiQ9ESW4
KOiK09881
OMAiTKAAHFF
OrthoDBiEOG091G06YD
PhylomeDBiQ9ESW4
TreeFamiTF320485

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ESW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG
60 70 80 90 100
NQLIPPNAQV KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTVVKT
110 120 130 140 150
DYEGQAKKLL ELMESTDVII VAGGDGTLQE VVTGVLRRTD EATFSKIPIG
160 170 180 190 200
FIPLGQTSSL SHTLFAESGN KVQHITDATL AIVKGETVPL DVLQIKGEKE
210 220 230 240 250
QPVYAMTGLR WGSFRDAGVK VSKYWYLGPL KTKAAHFFST LQEWPQTHQA
260 270 280 290 300
SISYTGPRER PPIEPEETPP RPSLYRRILR RLASFWAQPQ DASSREVSPE
310 320 330 340 350
VWKDVQLSTI ELSITTRNTQ LDLMSKEDFM NICIEPDTVS KGDFIIIGSK
360 370 380 390 400
KVRDPGLRAA GTECLQASHC TLVLPEGTEG SFSIDSEEYE AMPVEVKLLP
410 420
RKLRFFCDPR KREQMLPSTS Q
Length:421
Mass (Da):46,976
Last modified:March 1, 2001 - v1
Checksum:iDBBC48C9EB3206FD
GO
Isoform 2 (identifier: Q9ESW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-421: Missing.

Note: No experimental confirmation available.
Show »
Length:173
Mass (Da):18,899
Checksum:i54E2CB009875A90B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020927174 – 421Missing in isoform 2. 1 PublicationAdd BLAST248

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY772469 mRNA Translation: AAV38106.1
AJ401619 mRNA Translation: CAC06108.1
AK011715 mRNA Translation: BAB27796.1
AK076224 mRNA Translation: BAC36260.1
BC019145 mRNA Translation: AAH19145.1
BC093525 mRNA Translation: AAH93525.1
CCDSiCCDS20027.1 [Q9ESW4-1]
RefSeqiNP_076027.1, NM_023538.2 [Q9ESW4-1]
UniGeneiMm.32840

Genome annotation databases

EnsembliENSMUST00000031977; ENSMUSP00000031977; ENSMUSG00000029916 [Q9ESW4-1]
GeneIDi69923
KEGGimmu:69923
UCSCiuc009bmm.2 mouse [Q9ESW4-2]
uc009bmn.2 mouse [Q9ESW4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAGK_MOUSE
AccessioniPrimary (citable) accession number: Q9ESW4
Secondary accession number(s): Q9D087
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health