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Q9ESW4

- AGK_MOUSE

UniProt

Q9ESW4 - AGK_MOUSE

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Protein

Acylglycerol kinase, mitochondrial

Gene

Agk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.1 Publication

Catalytic activityi

ATP + acylglycerol = ADP + acyl-sn-glycerol 3-phosphate.
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Magnesium.By similarity

Kineticsi

  1. KM=45 µM for diacylglycerol1 Publication

Vmax=159 nmol/min/mg enzyme with dioeoylglycerol as substrate1 Publication

Pathwayi

GO - Molecular functioni

  1. acylglycerol kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. ceramide kinase activity Source: MGI
  4. diacylglycerol kinase activity Source: UniProtKB-EC
  5. lipid kinase activity Source: MGI
  6. NAD+ kinase activity Source: InterPro

GO - Biological processi

  1. ceramide biosynthetic process Source: MGI
  2. glycerolipid metabolic process Source: UniProtKB-UniPathway
  3. lipid phosphorylation Source: MGI
  4. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKQ9ESW4.
UniPathwayiUPA00230.

Names & Taxonomyi

Protein namesi
Recommended name:
Acylglycerol kinase, mitochondrial (EC:2.7.1.107, EC:2.7.1.94)
Alternative name(s):
Multiple substrate lipid kinase
Short name:
MuLK
Short name:
Multi-substrate lipid kinase
Gene namesi
Name:Agk
Synonyms:Mulk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1917173. Agk.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: MGI
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3131MitochondrionSequence AnalysisAdd
BLAST
Chaini32 – 421390Acylglycerol kinase, mitochondrialPRO_0000252381Add
BLAST

Proteomic databases

MaxQBiQ9ESW4.
PaxDbiQ9ESW4.
PRIDEiQ9ESW4.

PTM databases

PhosphoSiteiQ9ESW4.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiQ9ESW4.
CleanExiMM_AGK.
GenevestigatoriQ9ESW4.

Interactioni

Protein-protein interaction databases

BioGridi213759. 1 interaction.
IntActiQ9ESW4. 2 interactions.
MINTiMINT-4129364.

Structurei

3D structure databases

ProteinModelPortaliQ9ESW4.
SMRiQ9ESW4. Positions 59-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 199142DAGKcPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1597.
GeneTreeiENSGT00690000101761.
HOGENOMiHOG000252977.
HOVERGENiHBG080751.
InParanoidiQ9ESW4.
KOiK09881.
OMAiTKAAHFF.
OrthoDBiEOG7P2XS5.
PhylomeDBiQ9ESW4.
TreeFamiTF320485.

Family and domain databases

InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9ESW4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTAFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG
60 70 80 90 100
NQLIPPNAQV KKATVFLNPA ACKGKARTLF EKNAAPILHL SGMDVTVVKT
110 120 130 140 150
DYEGQAKKLL ELMESTDVII VAGGDGTLQE VVTGVLRRTD EATFSKIPIG
160 170 180 190 200
FIPLGQTSSL SHTLFAESGN KVQHITDATL AIVKGETVPL DVLQIKGEKE
210 220 230 240 250
QPVYAMTGLR WGSFRDAGVK VSKYWYLGPL KTKAAHFFST LQEWPQTHQA
260 270 280 290 300
SISYTGPRER PPIEPEETPP RPSLYRRILR RLASFWAQPQ DASSREVSPE
310 320 330 340 350
VWKDVQLSTI ELSITTRNTQ LDLMSKEDFM NICIEPDTVS KGDFIIIGSK
360 370 380 390 400
KVRDPGLRAA GTECLQASHC TLVLPEGTEG SFSIDSEEYE AMPVEVKLLP
410 420
RKLRFFCDPR KREQMLPSTS Q
Length:421
Mass (Da):46,976
Last modified:March 1, 2001 - v1
Checksum:iDBBC48C9EB3206FD
GO
Isoform 2 (identifier: Q9ESW4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-421: Missing.

Note: No experimental confirmation available.

Show »
Length:173
Mass (Da):18,899
Checksum:i54E2CB009875A90B
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei174 – 421248Missing in isoform 2. 1 PublicationVSP_020927Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY772469 mRNA. Translation: AAV38106.1.
AJ401619 mRNA. Translation: CAC06108.1.
AK011715 mRNA. Translation: BAB27796.1.
AK076224 mRNA. Translation: BAC36260.1.
BC019145 mRNA. Translation: AAH19145.1.
BC093525 mRNA. Translation: AAH93525.1.
CCDSiCCDS20027.1. [Q9ESW4-1]
RefSeqiNP_076027.1. NM_023538.2. [Q9ESW4-1]
UniGeneiMm.32840.

Genome annotation databases

EnsembliENSMUST00000031977; ENSMUSP00000031977; ENSMUSG00000029916. [Q9ESW4-1]
GeneIDi69923.
KEGGimmu:69923.
UCSCiuc009bmm.2. mouse. [Q9ESW4-2]
uc009bmn.2. mouse. [Q9ESW4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY772469 mRNA. Translation: AAV38106.1 .
AJ401619 mRNA. Translation: CAC06108.1 .
AK011715 mRNA. Translation: BAB27796.1 .
AK076224 mRNA. Translation: BAC36260.1 .
BC019145 mRNA. Translation: AAH19145.1 .
BC093525 mRNA. Translation: AAH93525.1 .
CCDSi CCDS20027.1. [Q9ESW4-1 ]
RefSeqi NP_076027.1. NM_023538.2. [Q9ESW4-1 ]
UniGenei Mm.32840.

3D structure databases

ProteinModelPortali Q9ESW4.
SMRi Q9ESW4. Positions 59-256.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 213759. 1 interaction.
IntActi Q9ESW4. 2 interactions.
MINTi MINT-4129364.

PTM databases

PhosphoSitei Q9ESW4.

Proteomic databases

MaxQBi Q9ESW4.
PaxDbi Q9ESW4.
PRIDEi Q9ESW4.

Protocols and materials databases

DNASUi 69923.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031977 ; ENSMUSP00000031977 ; ENSMUSG00000029916 . [Q9ESW4-1 ]
GeneIDi 69923.
KEGGi mmu:69923.
UCSCi uc009bmm.2. mouse. [Q9ESW4-2 ]
uc009bmn.2. mouse. [Q9ESW4-1 ]

Organism-specific databases

CTDi 55750.
MGIi MGI:1917173. Agk.

Phylogenomic databases

eggNOGi COG1597.
GeneTreei ENSGT00690000101761.
HOGENOMi HOG000252977.
HOVERGENi HBG080751.
InParanoidi Q9ESW4.
KOi K09881.
OMAi TKAAHFF.
OrthoDBi EOG7P2XS5.
PhylomeDBi Q9ESW4.
TreeFami TF320485.

Enzyme and pathway databases

UniPathwayi UPA00230 .
SABIO-RK Q9ESW4.

Miscellaneous databases

ChiTaRSi AGK. mouse.
NextBioi 330619.
PROi Q9ESW4.
SOURCEi Search...

Gene expression databases

Bgeei Q9ESW4.
CleanExi MM_AGK.
Genevestigatori Q9ESW4.

Family and domain databases

InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    Strain: FVB/N.
    Tissue: Carcinoma.
  2. "Further characterization of mammalian ceramide kinase: substrate delivery and (stereo)specificity, tissue distribution, and subcellular localization studies."
    Van Overloop H., Gijsbers S., Van Veldhoven P.P.
    J. Lipid Res. 47:268-283(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Head.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver and Mammary tumor.

Entry informationi

Entry nameiAGK_MOUSE
AccessioniPrimary (citable) accession number: Q9ESW4
Secondary accession number(s): Q9D087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3