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Protein

ATP-dependent RNA helicase DDX24

Gene

Ddx24

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase.Curated

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi238 – 2458ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. helicase activity Source: UniProtKB-KW
  3. poly(A) RNA binding Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX24 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 24
Gene namesi
Name:Ddx24
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1351337. Ddx24.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: MGI
  2. nucleolus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 857857ATP-dependent RNA helicase DDX24PRO_0000055030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801Phosphoserine2 Publications
Modified residuei92 – 921PhosphoserineBy similarity
Modified residuei288 – 2881PhosphoserineBy similarity
Modified residuei296 – 2961PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9ESV0.
PaxDbiQ9ESV0.
PRIDEiQ9ESV0.

PTM databases

PhosphoSiteiQ9ESV0.

Expressioni

Gene expression databases

BgeeiQ9ESV0.
CleanExiMM_DDX24.
ExpressionAtlasiQ9ESV0. baseline and differential.
GenevestigatoriQ9ESV0.

Interactioni

Protein-protein interaction databases

BioGridi205146. 2 interactions.
IntActiQ9ESV0. 1 interaction.
MINTiMINT-4092963.
STRINGi10090.ENSMUSP00000040890.

Structurei

3D structure databases

SMRiQ9ESV0. Positions 194-744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini225 – 528304Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini576 – 723148Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi193 – 22129Q motifAdd
BLAST
Motifi471 – 4744DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 877Poly-Glu
Compositional biasi755 – 7584Poly-Ala

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00550000074847.
HOGENOMiHOG000290702.
HOVERGENiHBG104200.
InParanoidiQ9ESV0.
KOiK14805.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9ESV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKETNSKP KLASRGTFQR KGIKIVGKWK QVTIDPNLFA DGQMDDLVCF
60 70 80 90 100
EELTDYRLVK NPSRLFSSEE TKKRKAQAVS EEEEEEEGQS SSPKKKIKLK
110 120 130 140 150
KQRDAARAAE GAAAQNEYEV KASEPEAQGE VTACSDQKVG GAKSESLAQA
160 170 180 190 200
APRKKKNKGK KKLDTFQSTS PKLPKKSKKT WMAEVHDQKA DVSAWRDLFV
210 220 230 240 250
PKAVLRALSF LGFSAPTPIQ ALTLAPAIRD KLDILGAAET GSGKTLAFAI
260 270 280 290 300
PMIHSVLQWH KMKAPPIPRS TGMPPREMRF GATAHLGSPC KDRTESGVLP
310 320 330 340 350
EEARIETEAQ PSDSGVQATP ETSASASAQT LLVCDDDAGE GPSSLEEKPV
360 370 380 390 400
PKQNEDGEEK FDAEQAGKLK QELCDQIAIY KVHPRRPLLG LVLTPTRELA
410 420 430 440 450
IQVRQHIDAV AKFTGINTAI LVGGMSTQKQ QRMLNRHPEI VIATPGRLWE
460 470 480 490 500
LVKEKHPHLS NLRQLRCLVI DEADRMVEKG HFAELSQLLE MLNDSQYNPS
510 520 530 540 550
RQTLVFSATL TLVHQAPARI LHKKHVKKMD KTDKLDLLMQ KVGMRGKPKV
560 570 580 590 600
IDLTRNEGTV ETLTETKIHC ETDEKDLYLY YFLMQYPGRS LVFANSISCI
610 620 630 640 650
KRLSGLLKVL DVMPLTLHAC MHQKQRLRNL EQFARLQDCV LLATDVAARG
660 670 680 690 700
LDIPKVQHVI HYQVPRTSEI YIHRSGRTAR AASEGLSLML IGPEDVINFK
710 720 730 740 750
KIYKTLQKDE DIPLFPVQSK YMDVVKERIR LARQIEKAEY RNFQACLHNS
760 770 780 790 800
WIEQAAAALE IELEEEMYKG GKADQQEERR RQKQMKMLKQ ELRHLLSQPL
810 820 830 840 850
FQENLKTRYP TQSGRPPQPV LASRNIESAL SCLSRQKRRR KKPKEPRAPP

QPGSSTS
Length:857
Mass (Da):96,429
Last modified:July 27, 2011 - v2
Checksum:iF3DCF47E9F27CAEC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti556 – 5572NE → ER in AAG02170 (PubMed:10936056).Curated
Sequence conflicti556 – 5572NE → ER in AAB01091 (Ref. 4) Curated
Sequence conflicti616 – 6161T → N in AAB01091 (Ref. 4) Curated
Sequence conflicti710 – 7101E → EDIPLFPVHFKKIYKTLQKD E in AAB01091 (Ref. 4) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214732 mRNA. Translation: AAG02170.1.
CH466549 Genomic DNA. Translation: EDL18835.1.
BC055048 mRNA. Translation: AAH55048.1.
BC055317 mRNA. Translation: AAH55317.1.
U46690 mRNA. Translation: AAB01091.1.
CCDSiCCDS26129.1.
RefSeqiNP_001152974.1. NM_001159502.1.
NP_065240.2. NM_020494.3.
XP_006515995.1. XM_006515932.2.
XP_006515996.1. XM_006515933.1.
UniGeneiMm.3935.
Mm.475067.

Genome annotation databases

EnsembliENSMUST00000044923; ENSMUSP00000040890; ENSMUSG00000041645.
GeneIDi27225.
KEGGimmu:27225.
UCSCiuc007ovj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF214732 mRNA. Translation: AAG02170.1.
CH466549 Genomic DNA. Translation: EDL18835.1.
BC055048 mRNA. Translation: AAH55048.1.
BC055317 mRNA. Translation: AAH55317.1.
U46690 mRNA. Translation: AAB01091.1.
CCDSiCCDS26129.1.
RefSeqiNP_001152974.1. NM_001159502.1.
NP_065240.2. NM_020494.3.
XP_006515995.1. XM_006515932.2.
XP_006515996.1. XM_006515933.1.
UniGeneiMm.3935.
Mm.475067.

3D structure databases

SMRiQ9ESV0. Positions 194-744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205146. 2 interactions.
IntActiQ9ESV0. 1 interaction.
MINTiMINT-4092963.
STRINGi10090.ENSMUSP00000040890.

PTM databases

PhosphoSiteiQ9ESV0.

Proteomic databases

MaxQBiQ9ESV0.
PaxDbiQ9ESV0.
PRIDEiQ9ESV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044923; ENSMUSP00000040890; ENSMUSG00000041645.
GeneIDi27225.
KEGGimmu:27225.
UCSCiuc007ovj.2. mouse.

Organism-specific databases

CTDi57062.
MGIiMGI:1351337. Ddx24.

Phylogenomic databases

eggNOGiCOG0513.
GeneTreeiENSGT00550000074847.
HOGENOMiHOG000290702.
HOVERGENiHBG104200.
InParanoidiQ9ESV0.
KOiK14805.

Miscellaneous databases

NextBioi305144.
PROiQ9ESV0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ESV0.
CleanExiMM_DDX24.
ExpressionAtlasiQ9ESV0. baseline and differential.
GenevestigatoriQ9ESV0.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of human DDX24 and mouse Ddx24, two novel putative DEAD-box proteins, and mapping DDX24 to human chromosome 14q32."
    Zhao Y., Yu L., Fu Q., Chen W., Jiang J., Gao J., Zhao S.
    Genomics 67:351-355(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6 and FVB/N-3.
    Tissue: Mammary tumor.
  4. "Identification of a lipopolysaccharide inducible gene in murine macrophages that putatively encodes an ATP-dependent RNA helicase."
    Drysdale B., Howard D.L., Johnson R.J.
    Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 399-857.
    Strain: BALB/c.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDDX24_MOUSE
AccessioniPrimary (citable) accession number: Q9ESV0
Secondary accession number(s): Q61119, Q7TM97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.