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Protein

NF-kappa-B inhibitor zeta

Gene

Nfkbiz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Seems also to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters. Promotes transcription of LCN2 and DEFB4. Is recruited to IL-6 promoters and activates IL-6 but decreases TNF-alpha production in response to LPS. Seems to be involved in the induction of inflammatory genes activated through TLR/IL-1 receptor signaling. May promote apoptosis (By similarity).By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor zeta
Alternative name(s):
I-kappa-B-zeta
Short name:
IkB-zeta
Short name:
IkappaBzeta
IL-1 inducible nuclear ankyrin-repeat protein
Short name:
INAP
Molecule possessing ankyrin repeats induced by lipopolysaccharide
Short name:
MAIL
Gene namesi
Name:Nfkbiz
Synonyms:Inap, Mail
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1931595. Nfkbiz.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice have splenocytes with defective proliferation in response to LPS but not to anti-CD40, IL-4 and anti-IgM. Though mice grew normally after birth, some of them started to develop atopic dermatitis-like skin lesions with acanthosis and lichenoid changes at the age of 4-5 weeks. All mice developed the disease by the age of 10 weeks. 5-week-old mice show pathological changes in the conjunctiva, including a heavy lymphocyte infiltration into the submucosa and loss of goblet cells in the conjunctival epithelium.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi163 – 1631K → A: Abolishes nuclear localozation. 1 Publication
Mutagenesisi164 – 1641R → A: Abolishes nuclear localozation. 1 Publication
Mutagenesisi177 – 1771K → A: Abolishes nuclear localozation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 728728NF-kappa-B inhibitor zetaPRO_0000323578Add
BLAST

Proteomic databases

MaxQBiQ9EST8.
PaxDbiQ9EST8.
PRIDEiQ9EST8.

PTM databases

iPTMnetiQ9EST8.
PhosphoSiteiQ9EST8.

Expressioni

Tissue specificityi

Expressed in kidney, liver, lung and heart. Expressed at very low levels in skeletal muscle, spleen and brain.1 Publication

Inductioni

By IL-1, LPS, peptidoglycan, bacterial lipoprotein, flagellin, MALP-2, R-848 and CpG DNA, but not by TNF-alpha.4 Publications

Gene expression databases

BgeeiENSMUSG00000035356.
GenevisibleiQ9EST8. MM.

Interactioni

Subunit structurei

Interacts with NFKB1/p50. Interacts with RELA; the interaction was not detected in (PubMed:11356851) (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Akirin2B1AXD83EBI-10107924,EBI-10107866

Protein-protein interaction databases

BioGridi219820. 5 interactions.
IntActiQ9EST8. 1 interaction.
STRINGi10090.ENSMUSP00000041173.

Structurei

3D structure databases

ProteinModelPortaliQ9EST8.
SMRiQ9EST8. Positions 449-720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati453 – 48230ANK 1Add
BLAST
Repeati489 – 51830ANK 2Add
BLAST
Repeati522 – 55130ANK 3Add
BLAST
Repeati561 – 58929ANK 4Add
BLAST
Repeati591 – 61727ANK 5Add
BLAST
Repeati622 – 65130ANK 6Add
BLAST
Repeati658 – 69134ANK 7Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 40375Required for transcriptional activityAdd
BLAST
Regioni414 – 728315Interaction with NFKB1/p50Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi163 – 17816Nuclear localization signalAdd
BLAST

Sequence similaritiesi

Contains 7 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOVERGENiHBG059690.
InParanoidiQ9EST8.
KOiK14242.
OMAiYEPNLFD.
OrthoDBiEOG091G06D9.
PhylomeDBiQ9EST8.
TreeFamiTF330224.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EST8-1) [UniParc]FASTAAdd to basket
Also known as: MAIL-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIVDKLLDDS RGGEGLLDAA GDCGLMTSPL NLAYFYGASP PSAPGAGDTG
60 70 80 90 100
YLSAVPSAPG SPGSDSSDFS STSSVSSCGA VESRPRGGAR AERPQVEPHM
110 120 130 140 150
GVGRQQRGPF QGVRVKNSVK ELLLHIRSNK QKASGQPVDE FKTQSVNIEQ
160 170 180 190 200
LTDLKSAVSA VGKRKGPDPL SDGPVCKRPA LLPSHFVTSP QTPTPGESME
210 220 230 240 250
DVRHSESKLD SSAALLQNII NIKNECNPVS LNTVQVSWMS PTVPQNSPRD
260 270 280 290 300
QCQDFHGGQA FSPPQKYQPF QVSGSPQMMD QASMYQYSPQ TQNMQQPPPL
310 320 330 340 350
PPQQQHQQNY PHNSPLQFSP YSRMSQSPKY DSNLFDTHEP QFCTGQSFVS
360 370 380 390 400
LLTGPGEPES LAVPVPAPTS IPPQTETQLQ TFSLMPSNAC EAVVGVHDVG
410 420 430 440 450
SHSLGTSLSL QNIMGSPMNT TQLGKSFFQW QVEQEESKLA NIPQDQFLAR
460 470 480 490 500
DGDGDTFLHI AVAQGRRALS YVLARKMNAL HMLDIKEHNG QSAFQVAVAA
510 520 530 540 550
NQHLIVQDLV NLGAQVNTTD CWGRTPLHVC AEKGHSQVLQ AIQKGAVRSN
560 570 580 590 600
QFVDLEATNY DGLTPLHCAV VAHNAVVHEL QRNRQSHSPE VQDLLLRNKS
610 620 630 640 650
LVDTIKCLIQ MGAAVEAKDR KSGRTALHLA AEEANLELIR LFLELPSCLS
660 670 680 690 700
FVNAKAYNGN TALHVAASLQ YRVTQLDAVR LLMRKGADPS TRNLENEQPV
710 720
HLVPDGPVGE QIRRILKGKS IQQRAPPY
Note: Major.
Length:728
Mass (Da):79,007
Last modified:March 1, 2001 - v1
Checksum:iE2CAB725295A08B7
GO
Isoform 2 (identifier: Q9EST8-2) [UniParc]FASTAAdd to basket
Also known as: MAIL-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Show »
Length:629
Mass (Da):69,179
Checksum:i8F87AA6147324534
GO
Isoform 3 (identifier: Q9EST8-3) [UniParc]FASTAAdd to basket
Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     236-429: Missing.

Show »
Length:534
Mass (Da):57,666
Checksum:iCC42F49D3E2E5995
GO

Sequence cautioni

The sequence BAE24648 differs from that shown. Reason: Frameshift at positions 216 and 227. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311N → K in BAE30878 (PubMed:16141072).Curated
Sequence conflicti87 – 871G → D in BAE35257 (PubMed:16141072).Curated
Sequence conflicti87 – 871G → D in BAE34809 (PubMed:16141072).Curated
Sequence conflicti142 – 1421K → E in BAE35257 (PubMed:16141072).Curated
Sequence conflicti142 – 1421K → E in BAE34809 (PubMed:16141072).Curated
Sequence conflicti208 – 2081K → E in BAE29631 (PubMed:16141072).Curated
Sequence conflicti223 – 2231K → R in BAE35601 (PubMed:16141072).Curated
Sequence conflicti239 – 2391M → I in BAE24648 (PubMed:11278262).Curated
Sequence conflicti273 – 2731S → G in BAE29631 (PubMed:16141072).Curated
Sequence conflicti461 – 4611A → V in BAE35257 (PubMed:16141072).Curated
Sequence conflicti461 – 4611A → V in BAE34809 (PubMed:16141072).Curated
Sequence conflicti475 – 4751R → K in BAE35257 (PubMed:16141072).Curated
Sequence conflicti475 – 4751R → K in BAE34809 (PubMed:16141072).Curated
Sequence conflicti600 – 6001S → G in BAE23017 (PubMed:11278262).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9999Missing in isoform 2. 1 PublicationVSP_032024Add
BLAST
Alternative sequencei236 – 429194Missing in isoform 3. 1 PublicationVSP_032025Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020974 mRNA. Translation: BAB18302.1.
AB040458 Genomic DNA. Translation: BAB85809.1.
AB026551 mRNA. Translation: BAA95161.2.
AB047549 mRNA. Translation: BAB32782.1.
AK082908 mRNA. Translation: BAC38681.1.
AK136509 mRNA. Translation: BAE23017.1.
AK141318 mRNA. Translation: BAE24648.1. Frameshift.
AK150519 mRNA. Translation: BAE29631.1.
AK152016 mRNA. Translation: BAE30878.1.
AK159093 mRNA. Translation: BAE34809.1.
AK159171 mRNA. Translation: BAE34871.1.
AK159459 mRNA. Translation: BAE35101.1.
AK159645 mRNA. Translation: BAE35257.1.
AK159917 mRNA. Translation: BAE35480.1.
AK160065 mRNA. Translation: BAE35601.1.
BC058188 mRNA. Translation: AAH58188.1.
AB196497 mRNA. Translation: BAD82955.1.
CCDSiCCDS28216.1. [Q9EST8-1]
CCDS49869.1. [Q9EST8-2]
RefSeqiNP_001152866.1. NM_001159394.1. [Q9EST8-1]
NP_001152867.1. NM_001159395.1. [Q9EST8-2]
NP_085115.1. NM_030612.3. [Q9EST8-1]
UniGeneiMm.247272.

Genome annotation databases

EnsembliENSMUST00000036273; ENSMUSP00000041173; ENSMUSG00000035356. [Q9EST8-1]
ENSMUST00000096026; ENSMUSP00000093726; ENSMUSG00000035356. [Q9EST8-2]
ENSMUST00000114457; ENSMUSP00000110101; ENSMUSG00000035356. [Q9EST8-2]
ENSMUST00000114458; ENSMUSP00000110102; ENSMUSG00000035356. [Q9EST8-1]
GeneIDi80859.
KEGGimmu:80859.
UCSCiuc007zlm.2. mouse. [Q9EST8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020974 mRNA. Translation: BAB18302.1.
AB040458 Genomic DNA. Translation: BAB85809.1.
AB026551 mRNA. Translation: BAA95161.2.
AB047549 mRNA. Translation: BAB32782.1.
AK082908 mRNA. Translation: BAC38681.1.
AK136509 mRNA. Translation: BAE23017.1.
AK141318 mRNA. Translation: BAE24648.1. Frameshift.
AK150519 mRNA. Translation: BAE29631.1.
AK152016 mRNA. Translation: BAE30878.1.
AK159093 mRNA. Translation: BAE34809.1.
AK159171 mRNA. Translation: BAE34871.1.
AK159459 mRNA. Translation: BAE35101.1.
AK159645 mRNA. Translation: BAE35257.1.
AK159917 mRNA. Translation: BAE35480.1.
AK160065 mRNA. Translation: BAE35601.1.
BC058188 mRNA. Translation: AAH58188.1.
AB196497 mRNA. Translation: BAD82955.1.
CCDSiCCDS28216.1. [Q9EST8-1]
CCDS49869.1. [Q9EST8-2]
RefSeqiNP_001152866.1. NM_001159394.1. [Q9EST8-1]
NP_001152867.1. NM_001159395.1. [Q9EST8-2]
NP_085115.1. NM_030612.3. [Q9EST8-1]
UniGeneiMm.247272.

3D structure databases

ProteinModelPortaliQ9EST8.
SMRiQ9EST8. Positions 449-720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219820. 5 interactions.
IntActiQ9EST8. 1 interaction.
STRINGi10090.ENSMUSP00000041173.

PTM databases

iPTMnetiQ9EST8.
PhosphoSiteiQ9EST8.

Proteomic databases

MaxQBiQ9EST8.
PaxDbiQ9EST8.
PRIDEiQ9EST8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036273; ENSMUSP00000041173; ENSMUSG00000035356. [Q9EST8-1]
ENSMUST00000096026; ENSMUSP00000093726; ENSMUSG00000035356. [Q9EST8-2]
ENSMUST00000114457; ENSMUSP00000110101; ENSMUSG00000035356. [Q9EST8-2]
ENSMUST00000114458; ENSMUSP00000110102; ENSMUSG00000035356. [Q9EST8-1]
GeneIDi80859.
KEGGimmu:80859.
UCSCiuc007zlm.2. mouse. [Q9EST8-1]

Organism-specific databases

CTDi64332.
MGIiMGI:1931595. Nfkbiz.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOVERGENiHBG059690.
InParanoidiQ9EST8.
KOiK14242.
OMAiYEPNLFD.
OrthoDBiEOG091G06D9.
PhylomeDBiQ9EST8.
TreeFamiTF330224.

Miscellaneous databases

ChiTaRSiNfkbiz. mouse.
PROiQ9EST8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035356.
GenevisibleiQ9EST8. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIKBZ_MOUSE
AccessioniPrimary (citable) accession number: Q9EST8
Secondary accession number(s): Q3TVL7
, Q3TWK9, Q3U8Y9, Q3UCI2, Q3URP0, Q3UW99, Q5NT97, Q99NA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.