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Protein

Proteasome assembly chaperone 2

Gene

Psmg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization (By similarity).By similarity

GO - Biological processi

  • mitotic spindle assembly checkpoint Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • proteasome assembly Source: UniProtKB
  • regulation of cell cycle Source: MGI

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome assembly chaperone 2
Alternative name(s):
CD40 ligand-activated specific transcript 3
Tumor necrosis factor superfamily member 5-induced protein 1
Gene namesi
Name:Psmg2Imported
Synonyms:Clast3Imported, Tnfsf5ip1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1922901. Psmg2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • nucleus Source: MGI
  • proteasome complex Source: GO_Central

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003225531 – 264Proteasome assembly chaperone 2Add BLAST264

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei137PhosphothreonineBy similarity1

Post-translational modificationi

Degraded by the proteasome upon completion of 20S proteasome maturation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9EST4.
PeptideAtlasiQ9EST4.
PRIDEiQ9EST4.

PTM databases

iPTMnetiQ9EST4.
PhosphoSitePlusiQ9EST4.

Expressioni

Developmental stagei

Expression is elevated in proliferating cells and down-regulated in cells undergoing growth arrest. During the cell cycle, expression is low in G1-arrested cells, increases during S phase and remains high at G2-M phase.1 Publication

Gene expression databases

BgeeiENSMUSG00000024537.
GenevisibleiQ9EST4. MM.

Interactioni

Subunit structurei

Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits (By similarity).By similarity

Protein-protein interaction databases

BioGridi223192. 1 interactor.
STRINGi10090.ENSMUSP00000025418.

Structurei

3D structure databases

ProteinModelPortaliQ9EST4.
SMRiQ9EST4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PSMG2 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3112. Eukaryota.
ENOG410XRHJ. LUCA.
GeneTreeiENSGT00390000018415.
HOGENOMiHOG000046033.
HOVERGENiHBG059464.
InParanoidiQ9EST4.
KOiK11876.
OMAiSWKSVYG.
OrthoDBiEOG091G0OFC.
PhylomeDBiQ9EST4.
TreeFamiTF105397.

Family and domain databases

InterProiView protein in InterPro
IPR019151. Proteasome_assmbl_chaperone_2.
IPR016562. Proteasome_assmbl_chp_2_euk.
PfamiView protein in Pfam
PF09754. PAC2. 1 hit.
PIRSFiPIRSF010044. UCP010044. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9EST4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFVPCGESVP DLTNFTLLMP AVSVGNVGQL AIDLIISTLN MCKIGYFYTD
60 70 80 90 100
CLVPMVGNNP YATEEENSNE LSINTEVYSL PSKKLVVLQL RSIFIKYKSK
110 120 130 140 150
SFCEKLLAWV ESSGCARIIV LSSSHSYHRN DAQLRSTPFR YLLTPCLQKS
160 170 180 190 200
VQNKIKSLNW LEMEKSRCIP EMSDSEFCIR IPGGGITKTL YDESCSKEIQ
210 220 230 240 250
MAVLLKFVSE GDNIPDAVSL VEYLNEWLQI IKPCNDGPMA SALPWKIPSS
260
WRLLFGSGLP PALF
Length:264
Mass (Da):29,524
Last modified:March 1, 2001 - v1
Checksum:i572AC760EDD7537E
GO
Isoform 22 Publications (identifier: Q9EST4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     97-161: YKSKSFCEKL...QNKIKSLNWL → VSMLAFLSSL...FILKEIVDYS
     162-264: Missing.

Show »
Length:161
Mass (Da):18,321
Checksum:iD3E1A23B650F551D
GO
Isoform 31 Publication (identifier: Q9EST4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:224
Mass (Da):25,351
Checksum:i9318299A602BCA1E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34L → Q in AAH16606 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0526911 – 40Missing in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_05269297 – 161YKSKS…SLNWL → VSMLAFLSSLMYGDRTQSLA LRIKTNFIILFYILRKGLDL IKNFIIFQVCYYHFIFILKE IVDYS in isoform 2. 2 PublicationsAdd BLAST65
Alternative sequenceiVSP_052693162 – 264Missing in isoform 2. 2 PublicationsAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031387 mRNA. Translation: BAB11962.1.
AK006069 mRNA. Translation: BAC25130.2.
AK077569 mRNA. Translation: BAC36869.1.
AK135578 mRNA. Translation: BAE22586.1.
AK167840 mRNA. Translation: BAE39861.1.
BC016606 mRNA. Translation: AAH16606.1.
CCDSiCCDS29324.1. [Q9EST4-1]
RefSeqiNP_598899.1. NM_134138.1. [Q9EST4-1]
UniGeneiMm.150701.

Genome annotation databases

EnsembliENSMUST00000025418; ENSMUSP00000025418; ENSMUSG00000024537. [Q9EST4-1]
GeneIDi107047.
KEGGimmu:107047.
UCSCiuc008fms.1. mouse. [Q9EST4-2]
uc008fmt.1. mouse. [Q9EST4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPSMG2_MOUSE
AccessioniPrimary (citable) accession number: Q9EST4
Secondary accession number(s): Q3TII5
, Q3UXH7, Q8C1R5, Q91YJ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2001
Last modified: May 10, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families