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Protein

Eukaryotic translation initiation factor 4E transporter

Gene

Eif4enif1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleoplasmic shuttling protein. Mediates the nuclear import of EIF4E by a piggy-back mechanism (By similarity).By similarity

GO - Molecular functioni

  • mRNA binding Source: MGI
  • poly(A) RNA binding Source: MGI

GO - Biological processi

  • negative regulation of neuron differentiation Source: MGI
  • negative regulation of translation Source: MGI
  • protein transport Source: UniProtKB-KW
  • stem cell population maintenance Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4E transporter
Short name:
4E-T
Short name:
eIF4E transporter
Alternative name(s):
Eukaryotic translation initiation factor 4E nuclear import factor 1
Gene namesi
Name:Eif4enif1
Synonyms:Clast4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1921453. Eif4enif1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity
  • Nucleus speckle By similarity
  • NucleusPML body By similarity

  • Note: Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner. Localization to nuclear foci and speckles requires active transcription (By similarity).By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic mRNA processing body Source: MGI
  • cytosol Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • membrane Source: MGI
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: MGI
  • PML body Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643821 – 983Eukaryotic translation initiation factor 4E transporterAdd BLAST983

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineBy similarity1
Modified residuei73PhosphoserineBy similarity1
Modified residuei77PhosphoserineBy similarity1
Modified residuei114PhosphoserineBy similarity1
Modified residuei119PhosphoserineBy similarity1
Modified residuei135PhosphoserineBy similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei344PhosphoserineBy similarity1
Modified residuei352PhosphoserineBy similarity1
Modified residuei416PhosphoserineBy similarity1
Modified residuei485N6-acetyllysineBy similarity1
Modified residuei563PhosphoserineCombined sources1
Modified residuei586PhosphoserineCombined sources1
Modified residuei692PhosphoserineBy similarity1
Modified residuei919PhosphoserineBy similarity1
Modified residuei949PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9EST3.
PaxDbiQ9EST3.
PeptideAtlasiQ9EST3.
PRIDEiQ9EST3.

PTM databases

iPTMnetiQ9EST3.
PhosphoSitePlusiQ9EST3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020454.
ExpressionAtlasiQ9EST3. baseline and differential.
GenevisibleiQ9EST3. MM.

Interactioni

Subunit structurei

Interacts with EIF4E. Interacts with importin beta only in presence of importin alpha, suggesting a direct interaction with importin alpha (By similarity). Interacts with APOBEC3G in an RNA-dependent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi216574. 5 interactors.
STRINGi10090.ENSMUSP00000105676.

Structurei

3D structure databases

ProteinModelPortaliQ9EST3.
SMRiQ9EST3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 35EIF4E-bindingBy similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi194 – 210Nuclear localization signalBy similarityAdd BLAST17
Motifi437 – 446Nuclear export signalBy similarity10
Motifi612 – 637Nuclear export signalBy similarityAdd BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi147 – 209Arg-richAdd BLAST63

Phylogenomic databases

eggNOGiENOG410IG0J. Eukaryota.
ENOG41110MN. LUCA.
GeneTreeiENSGT00390000012071.
HOGENOMiHOG000232370.
HOVERGENiHBG023298.
InParanoidiQ9EST3.
KOiK18728.
OMAiSMPTKVI.
OrthoDBiEOG091G04PY.
TreeFamiTF101531.

Family and domain databases

InterProiIPR018862. eIF4E_binding.
[Graphical view]
PfamiPF10477. EIF4E-T. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9EST3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKSVAETEN GDAFLELKKL PTSKSPHRYT KEELLDIKER PYSKQRPSCL
60 70 80 90 100
SEKYDSDGVW DPEKWHASLY PASGRSSPVE SLKKESESDR PSLVRRIADP
110 120 130 140 150
RERVKEDDLD VVLSPQRRSF GGGCHVTAAV SSRRSGSPLE KDSDGLRLLG
160 170 180 190 200
GRRIGSGRII SARAFEKDHR LSDKDLRDLR DRDRERDYKD KRFRREFGDS
210 220 230 240 250
KRVFGERRRN DSYTEEEPEW FSAGPTSQSE TIELTGFDDK ILEEDHKGRK
260 270 280 290 300
RTRRRTASVK EGIVECNGGV AEEDEVEVIL AQEPSADQEV PRDVILPEQS
310 320 330 340 350
PGEFDFNEFF NLDKVPCLAS MIEDVLGEGS VSASRFSRWF SNPSRSGSRS
360 370 380 390 400
SSLGSTPHEE LERLAGLEQA VLSPGQNSGN YFAPIPSEDH AENKVDILEM
410 420 430 440 450
LQKAKVDLKP LLSSLSANKE KLKESSHSGV VLSVEEVEAG LKGLKVDQQM
460 470 480 490 500
KNSTPFMAEH LEETLSAASS NRQLKKDGDM TAFNKLVNTM KASGTLPTQP
510 520 530 540 550
KVSRNVESHL LAPAEIPGQP VSKNILQELL GQPVQRPASS NLLSGLMGSL
560 570 580 590 600
EATASLLSQR APSPPMSQVF RTQAASADYL HPRIPSPIGF PSGPQQLLGD
610 620 630 640 650
PFQGMRKPMS PVSAQMSQLE LQQAALEGLA LPHDLAVQTA PFYQPGFSKP
660 670 680 690 700
QVDRTRDGLR NRQQRMSKSP APMHGGNSSS PAPAASITSM LSPSFTPTSV
710 720 730 740 750
IRKMYESREK TKEEMAPGMV VPGDGKEDTQ KTSEENLLSS NPIPNTDQDS
760 770 780 790 800
STTNPKLSTL QRSSCSTPLS QTSRYTKEQD YRPKTAGRKT PTLASPVPGT
810 820 830 840 850
PFLRPTHQVP LVPHVPIVRP AHQLHPGLVQ RLIAQGVHPQ HLPSLLQAGV
860 870 880 890 900
LPPGIDMAPL QGLSGPLLGQ PLYPLVSAAS HPLLNPRPGT PLHLAVMQQQ
910 920 930 940 950
LQRSVLHPPG SSSQAAAISV QTPQNVPSRS GMPHMHSQLE HRTSQRSSSP
960 970 980
VGLAKWFGSD VLQQPLPSMP TKVISVDELE YRQ
Length:983
Mass (Da):107,986
Last modified:July 27, 2011 - v2
Checksum:iC010A10376699DA8
GO
Isoform 2 (identifier: Q9EST3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     504-527: Missing.

Note: No experimental confirmation available.
Show »
Length:959
Mass (Da):105,393
Checksum:i8B4CC73C34EB492C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti345R → Q in BAB11963 (Ref. 1) Curated1
Sequence conflicti773S → N in BAB11963 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003785504 – 527Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031388 mRNA. Translation: BAB11963.1.
AL671968 Genomic DNA. Translation: CAI35201.1.
CH466574 Genomic DNA. Translation: EDL40398.1.
BC033410 mRNA. Translation: AAH33410.1.
AK012082 mRNA. Translation: BAB28016.1.
CCDSiCCDS24354.1. [Q9EST3-1]
CCDS48739.1. [Q9EST3-2]
RefSeqiNP_001160019.1. NM_001166547.1. [Q9EST3-1]
NP_001160020.1. NM_001166548.1. [Q9EST3-2]
NP_001160021.1. NM_001166549.1. [Q9EST3-2]
NP_076232.2. NM_023743.2. [Q9EST3-1]
XP_006514904.1. XM_006514841.2. [Q9EST3-1]
XP_006514905.1. XM_006514842.3. [Q9EST3-1]
XP_006514906.1. XM_006514843.3. [Q9EST3-1]
XP_006514907.1. XM_006514844.2. [Q9EST3-2]
XP_017170295.1. XM_017314806.1. [Q9EST3-2]
UniGeneiMm.255649.

Genome annotation databases

EnsembliENSMUST00000020734; ENSMUSP00000020734; ENSMUSG00000020454. [Q9EST3-2]
ENSMUST00000110048; ENSMUSP00000105675; ENSMUSG00000020454. [Q9EST3-2]
ENSMUST00000110049; ENSMUSP00000105676; ENSMUSG00000020454. [Q9EST3-1]
ENSMUST00000179770; ENSMUSP00000136768; ENSMUSG00000020454. [Q9EST3-1]
GeneIDi74203.
KEGGimmu:74203.
UCSCiuc007hsc.2. mouse. [Q9EST3-2]
uc007hse.2. mouse. [Q9EST3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB031388 mRNA. Translation: BAB11963.1.
AL671968 Genomic DNA. Translation: CAI35201.1.
CH466574 Genomic DNA. Translation: EDL40398.1.
BC033410 mRNA. Translation: AAH33410.1.
AK012082 mRNA. Translation: BAB28016.1.
CCDSiCCDS24354.1. [Q9EST3-1]
CCDS48739.1. [Q9EST3-2]
RefSeqiNP_001160019.1. NM_001166547.1. [Q9EST3-1]
NP_001160020.1. NM_001166548.1. [Q9EST3-2]
NP_001160021.1. NM_001166549.1. [Q9EST3-2]
NP_076232.2. NM_023743.2. [Q9EST3-1]
XP_006514904.1. XM_006514841.2. [Q9EST3-1]
XP_006514905.1. XM_006514842.3. [Q9EST3-1]
XP_006514906.1. XM_006514843.3. [Q9EST3-1]
XP_006514907.1. XM_006514844.2. [Q9EST3-2]
XP_017170295.1. XM_017314806.1. [Q9EST3-2]
UniGeneiMm.255649.

3D structure databases

ProteinModelPortaliQ9EST3.
SMRiQ9EST3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216574. 5 interactors.
STRINGi10090.ENSMUSP00000105676.

PTM databases

iPTMnetiQ9EST3.
PhosphoSitePlusiQ9EST3.

Proteomic databases

EPDiQ9EST3.
PaxDbiQ9EST3.
PeptideAtlasiQ9EST3.
PRIDEiQ9EST3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020734; ENSMUSP00000020734; ENSMUSG00000020454. [Q9EST3-2]
ENSMUST00000110048; ENSMUSP00000105675; ENSMUSG00000020454. [Q9EST3-2]
ENSMUST00000110049; ENSMUSP00000105676; ENSMUSG00000020454. [Q9EST3-1]
ENSMUST00000179770; ENSMUSP00000136768; ENSMUSG00000020454. [Q9EST3-1]
GeneIDi74203.
KEGGimmu:74203.
UCSCiuc007hsc.2. mouse. [Q9EST3-2]
uc007hse.2. mouse. [Q9EST3-1]

Organism-specific databases

CTDi56478.
MGIiMGI:1921453. Eif4enif1.

Phylogenomic databases

eggNOGiENOG410IG0J. Eukaryota.
ENOG41110MN. LUCA.
GeneTreeiENSGT00390000012071.
HOGENOMiHOG000232370.
HOVERGENiHBG023298.
InParanoidiQ9EST3.
KOiK18728.
OMAiSMPTKVI.
OrthoDBiEOG091G04PY.
TreeFamiTF101531.

Miscellaneous databases

ChiTaRSiEif4enif1. mouse.
PROiQ9EST3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020454.
ExpressionAtlasiQ9EST3. baseline and differential.
GenevisibleiQ9EST3. MM.

Family and domain databases

InterProiIPR018862. eIF4E_binding.
[Graphical view]
PfamiPF10477. EIF4E-T. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei4ET_MOUSE
AccessioniPrimary (citable) accession number: Q9EST3
Secondary accession number(s): Q8CFW0, Q9CSS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.