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Protein

Histamine N-methyltransferase

Gene

HNMT

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Inactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + histamine = S-adenosyl-L-homocysteine + N(tau)-methylhistamine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281SubstratePROSITE-ProRule annotation
Binding sitei60 – 601S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei89 – 891S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei94 – 941S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei120 – 1201S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei142 – 1421S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei283 – 2831SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  1. histamine N-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histamine N-methyltransferase (EC:2.1.1.8)
Short name:
HMT
Gene namesi
Name:HNMT
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447 Componenti: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. extracellular vesicular exosome Source: Ensembl
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Histamine N-methyltransferasePRO_0000084020Add
BLAST

Expressioni

Tissue specificityi

Expressed in jejunum, brain > lung, spleen, stomach > liver, kidney.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000002230.

Structurei

3D structure databases

ProteinModelPortaliQ9EST2.
SMRiQ9EST2. Positions 5-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. HNMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG39680.
GeneTreeiENSGT00390000002862.
HOGENOMiHOG000231790.
HOVERGENiHBG051914.
InParanoidiQ9EST2.
OMAiMDISDCF.
OrthoDBiEOG7Z69CQ.
TreeFamiTF331080.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016673. Histamine_N-methyltransferase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF016616. HHMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51597. SAM_HNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9EST2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSMRSLFS DHGRYFEAFR RFLNNSTEYQ CMREFMDKQL PGIIARIGGS
60 70 80 90 100
KSEIKVLSIG GGAGEMDLHI LSKVKAQYPG VHIINEVVEP SAEQITKYKE
110 120 130 140 150
LVAKTSNLEN IKFAWHKETS SEYQNRVMEQ KEIQKWDFIH MIQMLYYVDD
160 170 180 190 200
IPATLKFFHS LLATNAKILI ILVSGKSGWL KFWKKYRSRL PQNDLCQYVT
210 220 230 240 250
SFDIIQMLDS LGIKYQCYDL LSTMDITDCF IDGNENGELL WDFLTETCNF
260 270 280 290
LTTAPPDLRA EIMKDLQGPE FIVRKEGKIL FDNSLSFITI EA
Length:292
Mass (Da):33,712
Last modified:February 28, 2001 - v1
Checksum:i1D7F79D84C11BF40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032693 mRNA. Translation: BAB13758.1.
RefSeqiNP_001166431.1. NM_001172960.2.

Genome annotation databases

EnsembliENSCPOT00000002482; ENSCPOP00000002230; ENSCPOG00000002449.
GeneIDi100135537.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032693 mRNA. Translation: BAB13758.1.
RefSeqiNP_001166431.1. NM_001172960.2.

3D structure databases

ProteinModelPortaliQ9EST2.
SMRiQ9EST2. Positions 5-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000002230.

Chemistry

ChEMBLiCHEMBL4485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000002482; ENSCPOP00000002230; ENSCPOG00000002449.
GeneIDi100135537.

Organism-specific databases

CTDi3176.

Phylogenomic databases

eggNOGiNOG39680.
GeneTreeiENSGT00390000002862.
HOGENOMiHOG000231790.
HOVERGENiHBG051914.
InParanoidiQ9EST2.
OMAiMDISDCF.
OrthoDBiEOG7Z69CQ.
TreeFamiTF331080.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR016673. Histamine_N-methyltransferase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PIRSFiPIRSF016616. HHMT. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51597. SAM_HNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Guinea pig histamine N-methyltransferase: cDNA cloning and mRNA distribution."
    Kitanaka J., Kitanaka N., Tsujimura T., Kakihana M., Terada N., Takemura M.
    Jpn. J. Pharmacol. 85:105-108(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: Hartley.
    Tissue: Brain.

Entry informationi

Entry nameiHNMT_CAVPO
AccessioniPrimary (citable) accession number: Q9EST2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2004
Last sequence update: February 28, 2001
Last modified: January 6, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.