Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pro-FMRFamide-related neuropeptide VF

Gene

Npvf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP (By similarity). Neuropeptide NPVF blocks morphine-induced analgesia.By similarity1 Publication

GO - Molecular functioni

  • receptor binding Source: RGD

GO - Biological processi

  • neuropeptide signaling pathway Source: RGD
  • sensory perception of pain Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Neuropeptide

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-FMRFamide-related neuropeptide VF
Alternative name(s):
FMRFamide-related peptides
Cleaved into the following 3 chains:
Alternative name(s):
Neuropeptide RFRP-2
Gene namesi
Name:Npvf
Synonyms:Rfrp
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61815. Npvf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Propeptidei27 – 5731PRO_0000009932Add
BLAST
Peptidei58 – 9437Neuropeptide NPSFPRO_0000009933Add
BLAST
Peptidei83 – 9412Neuropeptide RFRP-1Sequence analysisPRO_0000401172Add
BLAST
Propeptidei97 – 10610PRO_0000009934
Peptidei108 – 12518Neuropeptide NPVFPRO_0000009935Add
BLAST
Propeptidei128 – 20376PRO_0000009936Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei94 – 941Phenylalanine amideBy similarity
Modified residuei125 – 1251Phenylalanine amide

Keywords - PTMi

Amidation, Cleavage on pair of basic residues

Proteomic databases

PaxDbiQ9ESQ9.

Expressioni

Tissue specificityi

Isoform 1 is expressed at high levels in the hypothalamus and eye. Isoform 2 is specifically expressed in a region between the dorsomedial hypothalamic and ventromedial hypothalamic nuclei.2 Publications

Interactioni

GO - Molecular functioni

  • receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014437.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IWCW. Eukaryota.
ENOG410Z21X. LUCA.
HOGENOMiHOG000133054.
HOVERGENiHBG031581.
InParanoidiQ9ESQ9.
PhylomeDBiQ9ESQ9.

Family and domain databases

InterProiIPR026297. FMRFamide-related/fGRP.
[Graphical view]
PANTHERiPTHR14403. PTHR14403. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9ESQ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIISSKRFI LLTLATSSFL TSNTLCSDEL MMPHFHSKEG YGKYYQLRGI
60 70 80 90 100
PKGVKERSVT FQELKDWGAK KDIKMSPAPA NKVPHSAANL PLRFGRNIED
110 120 130 140 150
RRSPRARANM EAGTMSHFPS LPQRFGRTTA RRITKTLAGL PQKSLHSLAS
160 170 180 190 200
SELLYAMTRQ HQEIQSPGQE QPRKRVFTET DDAERKQEKI GNLQPVLQGA

MKL
Length:203
Mass (Da):22,979
Last modified:March 1, 2001 - v1
Checksum:iB5F0F26E852BE214
GO
Isoform 2 (identifier: Q9ESQ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     174-203: Missing.

Show »
Length:173
Mass (Da):19,553
Checksum:i95A9AC1E20BA9772
GO

Mass spectrometryi

Molecular mass is 1010.54 Da from positions 108 - 125. Determined by ESI. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei174 – 20330Missing in isoform 2. 1 PublicationVSP_039964Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040288 mRNA. Translation: BAB17672.1.
AF330059 mRNA. Translation: AAK94203.2.
RefSeqiNP_076442.1. NM_023952.1. [Q9ESQ9-1]
UniGeneiRn.62354.

Genome annotation databases

GeneIDi60570.
KEGGirno:60570.
UCSCiRGD:61815. rat. [Q9ESQ9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040288 mRNA. Translation: BAB17672.1.
AF330059 mRNA. Translation: AAK94203.2.
RefSeqiNP_076442.1. NM_023952.1. [Q9ESQ9-1]
UniGeneiRn.62354.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014437.

Proteomic databases

PaxDbiQ9ESQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60570.
KEGGirno:60570.
UCSCiRGD:61815. rat. [Q9ESQ9-1]

Organism-specific databases

CTDi64111.
RGDi61815. Npvf.

Phylogenomic databases

eggNOGiENOG410IWCW. Eukaryota.
ENOG410Z21X. LUCA.
HOGENOMiHOG000133054.
HOVERGENiHBG031581.
InParanoidiQ9ESQ9.
PhylomeDBiQ9ESQ9.

Miscellaneous databases

PROiQ9ESQ9.

Family and domain databases

InterProiIPR026297. FMRFamide-related/fGRP.
[Graphical view]
PANTHERiPTHR14403. PTHR14403. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Identification and characterization of novel mammalian neuropeptide FF-like peptides that attenuate morphine-induced antinociception."
    Liu Q., Guan X.-M., Martin W.J., McDonald T.P., Clements M.K., Jiang Q., Zeng Z., Jacobson M., Williams D.L. Jr., Yu H., Bomford D., Figueroa D., Mallee J., Wang R., Evans J., Gould R., Austin C.P.
    J. Biol. Chem. 276:36961-36969(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. "A novel rat hypothalamic RFamide-related peptide identified by immunoaffinity chromatography and mass spectrometry."
    Ukena K., Iwakoshi E., Minakata H., Tsutsui K.
    FEBS Lett. 512:255-258(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 108-125, SYNTHESIS, MASS SPECTROMETRY.
    Strain: Donryu.
    Tissue: Hypothalamus.

Entry informationi

Entry nameiNPVF_RAT
AccessioniPrimary (citable) accession number: Q9ESQ9
Secondary accession number(s): Q920A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.