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Q9ESP7

- ATS4_RAT

UniProt

Q9ESP7 - ATS4_RAT

Protein

A disintegrin and metalloproteinase with thrombospondin motifs 4

Gene

Adamts4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (05 Dec 2001)
      Previous versions | rss
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    Functioni

    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

    Catalytic activityi

    Glutamyl endopeptidase; bonds cleaved include 370-Thr-Glu-Gly-Glu-|-Ala-Arg-Gly-Ser-377 in the interglobular domain of mammalian aggrecan.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi154 – 1541Zinc; catalyticBy similarity
    Active sitei155 – 1551PROSITE-ProRule annotation
    Metal bindingi158 – 1581Zinc; catalyticBy similarity
    Metal bindingi164 – 1641Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metalloendopeptidase activity Source: InterPro
    2. metallopeptidase activity Source: RGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycoprotein catabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM12.221.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A disintegrin and metalloproteinase with thrombospondin motifs 4 (EC:3.4.24.82)
    Short name:
    ADAM-TS 4
    Short name:
    ADAM-TS4
    Short name:
    ADAMTS-4
    Alternative name(s):
    Aggrecanase-1
    Gene namesi
    Name:Adamts4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621242. Adamts4.

    Subcellular locationi

    GO - Cellular componenti

    1. proteinaceous extracellular matrix Source: RGD

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei‹1 – 5›5By similarityPRO_0000029168
    Chaini6 – 630625A disintegrin and metalloproteinase with thrombospondin motifs 4PRO_0000029169Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi86 ↔ 138By similarity
    Glycosylationi96 – 961N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi115 ↔ 120By similarity
    Disulfide bondi132 ↔ 216By similarity
    Disulfide bondi170 ↔ 200By similarity
    Disulfide bondi242 ↔ 265By similarity
    Disulfide bondi253 ↔ 275By similarity
    Disulfide bondi260 ↔ 294By similarity
    Disulfide bondi288 ↔ 299By similarity
    Disulfide bondi325 ↔ 362By similarity
    Disulfide bondi329 ↔ 367By similarity
    Disulfide bondi340 ↔ 352By similarity
    Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The precursor is cleaved by a furin endopeptidase.
    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion By similarity.By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiQ9ESP7.
    PRIDEiQ9ESP7.

    Expressioni

    Tissue specificityi

    Brain specific.

    Gene expression databases

    GenevestigatoriQ9ESP7.

    Interactioni

    Subunit structurei

    Interacts with SRPX2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9ESP7.
    SMRiQ9ESP7. Positions 8-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 221211Peptidase M12BPROSITE-ProRule annotationAdd
    BLAST
    Domaini233 – 30371DisintegrinAdd
    BLAST
    Domaini313 – 36856TSP type-1PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni479 – 630152SpacerAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi40 – 456Poly-Ala
    Compositional biasi368 – 478111Cys-richAdd
    BLAST

    Domaini

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.

    Sequence similaritiesi

    Contains 1 disintegrin domain.Curated
    Contains 1 peptidase M12B domain.PROSITE-ProRule annotation
    Contains 1 TSP type-1 domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG303167.
    HOVERGENiHBG004313.
    KOiK07764.
    PhylomeDBiQ9ESP7.

    Family and domain databases

    Gene3Di3.40.390.10. 1 hit.
    InterProiIPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view]
    PfamiPF05986. ADAM_spacer1. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view]
    PRINTSiPR01857. ADAMTSFAMILY.
    SMARTiSM00209. TSP1. 1 hit.
    [Graphical view]
    SUPFAMiSSF82895. SSF82895. 1 hit.
    PROSITEiPS50215. ADAM_MEPRO. 1 hit.
    PS50092. TSP1. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9ESP7-1 [UniParc]FASTAAdd to Basket

    « Hide

    RRTKRFASLS RFVETLVVAD DKMAAFHGAG LKHYLLTVMA AAAKAFKHPS    50
    IRNPVNLVVT RLVILGSGQE VPQVGPSAAQ TLRSFCTWQK GLNPPNDSDP 100
    DHFDTAILFT RQDLCGVSTC DALGMAGVGT VCDPARSCAI VEDDGLQSAF 150
    TAAHELGHVF NMLHDNSKPC ANLNGQGSSS RHVMAPVMAH VDPEEPWSPC 200
    SARFITDFLD NGYGHCLLDK PEAPLHLPVT FPGKDYDADR QCQLTFGPDS 250
    SHCPQLPPPC AALWCFGHLN GHAMCQTKHS PWADGTPCGP AQACMGGRCL 300
    HVDQLKDFNI PQAGGWGPWG PWGDCSRTCG GGVQFSSRDC TKPVPRNGGK 350
    YCEGRRTPFR SCNTKNCPHG SALTFREEQC AAYNHRTDLF KSFPGPMDWV 400
    PRYTGVAPRD QCKLTCQARA LGYYYVLEPR VADGTPCSPD SSSVCVQGRC 450
    IHAGCDRIIG SKKKFDKCMV CGGNGSSCSK QSGSFKKFRY GYSDVVTIPA 500
    GRTHILVRQQ GGSGLKSIYL ALKLADGSYA LNGEYTLMPS STDVVLPGAV 550
    SLRYSGRTAA SETLSGHGPL AQPLTLQVLV AGNPQNVRLR YSFFVPRPVP 600
    STPRPPPQNW LQRRAEILEI LRKRTWAGRK 630
    Length:630
    Mass (Da):68,385
    Last modified:December 5, 2001 - v2
    Checksum:i63A428753167C7EF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB042272 mRNA. Translation: BAB16474.1.
    AB042271 mRNA. Translation: BAB16473.1.
    AB042273 mRNA. Translation: BAB16475.1.
    RefSeqiNP_076449.1. NM_023959.1.
    UniGeneiRn.64490.

    Genome annotation databases

    GeneIDi66015.
    KEGGirno:66015.
    UCSCiRGD:621242. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB042272 mRNA. Translation: BAB16474.1 .
    AB042271 mRNA. Translation: BAB16473.1 .
    AB042273 mRNA. Translation: BAB16475.1 .
    RefSeqi NP_076449.1. NM_023959.1.
    UniGenei Rn.64490.

    3D structure databases

    ProteinModelPortali Q9ESP7.
    SMRi Q9ESP7. Positions 8-300.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M12.221.

    Proteomic databases

    PaxDbi Q9ESP7.
    PRIDEi Q9ESP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 66015.
    KEGGi rno:66015.
    UCSCi RGD:621242. rat.

    Organism-specific databases

    CTDi 9507.
    RGDi 621242. Adamts4.

    Phylogenomic databases

    eggNOGi NOG303167.
    HOVERGENi HBG004313.
    KOi K07764.
    PhylomeDBi Q9ESP7.

    Miscellaneous databases

    NextBioi 614286.
    PROi Q9ESP7.

    Gene expression databases

    Genevestigatori Q9ESP7.

    Family and domain databases

    Gene3Di 3.40.390.10. 1 hit.
    InterProi IPR010294. ADAM_spacer1.
    IPR024079. MetalloPept_cat_dom.
    IPR001590. Peptidase_M12B.
    IPR013273. Peptidase_M12B_ADAM-TS.
    IPR000884. Thrombospondin_1_rpt.
    [Graphical view ]
    Pfami PF05986. ADAM_spacer1. 1 hit.
    PF01421. Reprolysin. 1 hit.
    PF00090. TSP_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01857. ADAMTSFAMILY.
    SMARTi SM00209. TSP1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF82895. SSF82895. 1 hit.
    PROSITEi PS50215. ADAM_MEPRO. 1 hit.
    PS50092. TSP1. 1 hit.
    PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ADAMTS-4 (a disintegrin and metalloproteinase with thrombospondin motifs) is transcriptionally induced in beta-amyloid treated rat astrocytes."
      Satoh K., Suzuki N., Yokota H.
      Neurosci. Lett. 289:177-180(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.

    Entry informationi

    Entry nameiATS4_RAT
    AccessioniPrimary (citable) accession number: Q9ESP7
    Secondary accession number(s): Q9ESP6, Q9ESP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: December 5, 2001
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3