Q9ESN9 (JIP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-Jun-amino-terminal kinase-interacting protein 3
Short name=JIP-3
Short name=JNK-interacting protein 3

Alternative name(s):
JNK MAP kinase scaffold protein 3
JNK/SAPK-associated protein 1
Short name=JSAP1
Mitogen-activated protein kinase 8-interacting protein 3
Sunday driver 2

Gene names

Name:	Mapk8ip3
Synonyms:	Jip3, Jsap1, Syd2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Ref.1 Ref.3
Subunit structure	Forms homo- or heterooligomeric complexes. The central region of MAPK8IP3 interacts with the C-terminal of MAPK8IP2 but not MAPK8IP1. Binds specific components of the JNK signaling pathway namely MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3 to the N-terminal region, MAP2K4/MKK4 and MAP2K7/MKK7 to the central region and MAP3K11 to the C-terminal region. Binds the TPR motif-containing C-terminal of kinesin light chain, pre-assembled MAPK8IP1 scaffolding complexes are then transported as a cargo of kinesin, to the required subcellular location. Interacts with ROCK1 and this interaction is enhanced by ultraviolet-B (UVB) radiation By similarity. Ref.1 Ref.3 Ref.4 Ref.6
Subcellular location	Cytoplasm. Golgi apparatus. Cytoplasmic vesicle. Cell projection › growth cone. Note: Localized in the soma and growth cones of differentiated neurites and the Golgi and vesicles of the early secretory compartment of epithelial cells. Ref.3 Ref.4
Tissue specificity	Highly expressed throughout many regions of the brain and at lower levels in the heart, liver, lung, testes and kidney. All isoforms have been identified in the brain, isoform 1a is also expressed in the spleen and lung. Ref.2 Ref.3
Induction	Expressed in neurites 5 days following initiation of nerve growth factor induced differentiation. NGF withdrawal results in the down-regulation of MAPK8IP3 protein by caspase-mediated cleavage. Ref.3
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR Phosphorylation by ROCK1 is crucial for the recruitment of JNK By similarity. Ref.1 Ref.3
Sequence similarities	Belongs to the JIP scaffold family.
Sequence caution	The sequence AAG36931.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm Cytoplasmic vesicle Golgi apparatus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of JUN kinase activity Inferred from direct assay PubMed 15767678. Source: MGI axon guidance Inferred from mutant phenotype PubMed 15572149. Source: MGI forebrain development Inferred from mutant phenotype PubMed 12897243PubMed 15572149. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 18324732. Source: MGI lung alveolus development Inferred from mutant phenotype PubMed 18324732. Source: MGI lung morphogenesis Inferred from mutant phenotype PubMed 18324732. Source: MGI post-embryonic development Inferred from mutant phenotype PubMed 18324732. Source: MGI protein localization Inferred from mutant phenotype PubMed 15572149. Source: MGI regulation of gene expression Inferred from direct assay PubMed 18324732. Source: MGI respiratory gaseous exchange Inferred from mutant phenotype PubMed 12897243. Source: MGI vesicle-mediated transport Inferred from direct assay Ref.4. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from direct assay Ref.4. Source: UniProtKB axolemma Inferred from direct assay PubMed 16606357. Source: MGI cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from direct assay PubMed 16606357. Source: MGI growth cone Inferred from electronic annotation. Source: UniProtKB-SubCell smooth endoplasmic reticulum Inferred from direct assay PubMed 16606357. Source: MGI
Molecular_function	MAP-kinase scaffold activity Inferred from direct assay Ref.1. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Klc1	O88447	3	EBI-301496,EBI-301550
Klc2	O88448	4	EBI-301496,EBI-301558

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1c (identifier: Q9ESN9-1)

Also known as: 3b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1a (identifier: Q9ESN9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: Missing.
     219-249: Missing.

Isoform 1b (identifier: Q9ESN9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: S → SPRQSWRKS
     219-249: Missing.

Isoform 1d (identifier: Q9ESN9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: Missing.

Isoform 3a (identifier: Q9ESN9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     410-415: Missing.
     505-513: Missing.

Isoform 1e (identifier: Q9ESN9-6)

The sequence of this isoform differs from the canonical sequence as follows:
     201-201: S → SPRQSWRKS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1337	1337	C-Jun-amino-terminal kinase-interacting protein 3		PRO_0000220634
Regions
	Coiled coil	58 – 177	120	Potential
	Coiled coil	437 – 555	119	Potential
Amino acid modifications
	Modified residue	266	1	Phosphothreonine; by MAPK Ref.1 Ref.3
	Modified residue	276	1	Phosphothreonine; by MAPK Ref.1 Ref.3
	Modified residue	287	1	Phosphothreonine; by MAPK Ref.1 Ref.3
	Modified residue	315	1	Phosphoserine; by ROCK1 By similarity
	Modified residue	365	1	Phosphoserine; by ROCK1 By similarity
	Modified residue	366	1	Phosphoserine; by ROCK1 By similarity
	Modified residue	677	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	201	1	Missing in isoform 1a and isoform 1d. Ref.1 Ref.2		VSP_002775
	Alternative sequence	201	1	S → SPRQSWRKS in isoform 1b and isoform 1e. Ref.2		VSP_002776
	Alternative sequence	219 – 249	31	Missing in isoform 1a and isoform 1b. Ref.1 Ref.2		VSP_002777
	Alternative sequence	410 – 415	6	Missing in isoform 3a. Ref.3		VSP_002778
	Alternative sequence	505 – 513	9	Missing in isoform 3a. Ref.3		VSP_002779
Experimental info
	Mutagenesis	205	1	R → G: Results in inhibition of JNK binding. Ref.3
	Mutagenesis	206	1	P → G: Results in inhibition of JNK binding. Ref.3
	Mutagenesis	207	1	T → G: Results in inhibition of JNK binding. Ref.3
	Mutagenesis	208	1	S → G: Results in inhibition of JNK binding. Ref.3
	Mutagenesis	209	1	L → G: Results in inhibition of JNK binding. Ref.3
	Mutagenesis	266	1	T → A: Results in loss of phosphorylation of MAPK8IP3; when associated with A-276 and A-287. Does not effect binding of components of the JNK pathway. Ref.3
	Mutagenesis	276	1	T → A: Results in loss of phosphorylation of MAPK8IP3; when associated with A-266 and A-287. Does not effect binding of components of the JNK pathway. Ref.3
	Mutagenesis	287	1	T → A: Results in loss of phosphorylation of MAPK8IP3; when associated with A-266 and A-287. Does not effect binding of components of the JNK pathway. Ref.3
	Sequence conflict	312	1	K → R in AAG36931. Ref.4
	Sequence conflict	376	1	F → L in AAF26843. Ref.3
	Sequence conflict	561	1	E → K in AAG36931. Ref.4
	Sequence conflict	1272	1	A → V in AAG36931. Ref.4
	Sequence conflict	1322	1	A → D in AAG36931. Ref.4
	Sequence conflict	1325	1	S → T in AAG36931. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1c (3b) [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D7DEE54C17B74106	FASTA	1,337	147,561
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR SRKERPTSLN VFPLADGMVR AQMGGKLVPA GDHWHLSDLG 250 260 270 280 290 300 QLQSSSSYQC PNDEMSESGQ SSAAATPSTT GTKSNTPTSS VPSAAVTPLN ESLQPLGDYV 310 320 330 340 350 360 SVTKNNKQAR EKRNSRNMEV QVTQEMRNVS IGMGSSDEWS DVQDIIDSTP ELDVCPETRL 370 380 390 400 410 420 ERTGSSPTQG IVNKAFGINT DSLYHELSTA GSEVIGDVDE GADLLGEFSV RDDFFGMGKE 430 440 450 460 470 480 VGNLLLENSQ LLETKNALNV VKNDLIAKVD QLSGEQEVLK GELEAAKQAK VKLENRIKEL 490 500 510 520 530 540 EEELKRVKSE AVTARREPRE EVEDVSSYLC TELDKIPMAQ RRRFTRVEMA RVLMERNQYK 550 560 570 580 590 600 ERLMELQEAV RWTEMIRASR EHPSVQEKKK STIWQFFSRL FSSSSSPPPA KRSYPSVNIH 610 620 630 640 650 660 YKSPTAAGFS QRRSHALCQI SAGSRPLEFF PDDDCTSSAR REQKREQYRQ VREHVRNDDG 670 680 690 700 710 720 RLQACGWSLP AKYKQLSPNG GQEDTRMKNV PVPVYCRPLV EKDPSTKLWC AAGVNLSGWK 730 740 750 760 770 780 PHEEDSSNGP KPVPGRDPLT CDREGEGEPK STHPSPEKKK AKETPEADAT SSRVWILTST 790 800 810 820 830 840 LTTSKVVIID ANQPGTIVDQ FTVCNAHVLC ISSIPAASDS DYPPGEMFLD SDVNPEDSGA 850 860 870 880 890 900 DGVLAGITLV GCATRCNVPR SNCSSRGDTP VLDKGQGDVA TTANGKVNPS QSTEEATEAT 910 920 930 940 950 960 EVPDPGPSES EATTVRPGPL TEHVFTDPAP TPSSSTQPAS ENGSESNGTI VQPQVEPSGE 970 980 990 1000 1010 1020 LSTTTSSAAP TMWLGAQNGW LYVHSAVANW KKCLHSIKLK DSVLSLVHVK GRVLVALADG 1030 1040 1050 1060 1070 1080 TLAIFHRGED GQWDLSNYHL MDLGHPHHSI RCMAVVNDRV WCGYKNKVHV IQPKTMQIEK 1090 1100 1110 1120 1130 1140 SFDAHPRRES QVRQLAWIGD GVWVSIRLDS TLRLYHAHTH QHLQDVDIEP YVSKMLGTGK 1150 1160 1170 1180 1190 1200 LGFSFVRITA LLIAGNRLWV GTGNGVVISI PLTETVVLHR GQLLGLRANK TSPTSGEGTR 1210 1220 1230 1240 1250 1260 PGGIIHVYGD DSSDKAASSF IPYCSMAQAQ LCFHGHRDAV KFFVSVPGNV LATLNGSVLD 1270 1280 1290 1300 1310 1320 SPSEGPGPAA PAADAEGQKL KNALVLSGGE GYIDFRIGDG EDDETEECAG DVNQTKPSLS 1330 KAERSHIIVW QVSYTPE
	Isoform 1a [UniParc]. Checksum: 0BF7257DCFF6D236	FASTA	1,305	144,138
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR RKERPTSLNV FPLADGMCPN DEMSESGQSS AAATPSTTGT 250 260 270 280 290 300 KSNTPTSSVP SAAVTPLNES LQPLGDYVSV TKNNKQAREK RNSRNMEVQV TQEMRNVSIG 310 320 330 340 350 360 MGSSDEWSDV QDIIDSTPEL DVCPETRLER TGSSPTQGIV NKAFGINTDS LYHELSTAGS 370 380 390 400 410 420 EVIGDVDEGA DLLGEFSVRD DFFGMGKEVG NLLLENSQLL ETKNALNVVK NDLIAKVDQL 430 440 450 460 470 480 SGEQEVLKGE LEAAKQAKVK LENRIKELEE ELKRVKSEAV TARREPREEV EDVSSYLCTE 490 500 510 520 530 540 LDKIPMAQRR RFTRVEMARV LMERNQYKER LMELQEAVRW TEMIRASREH PSVQEKKKST 550 560 570 580 590 600 IWQFFSRLFS SSSSPPPAKR SYPSVNIHYK SPTAAGFSQR RSHALCQISA GSRPLEFFPD 610 620 630 640 650 660 DDCTSSARRE QKREQYRQVR EHVRNDDGRL QACGWSLPAK YKQLSPNGGQ EDTRMKNVPV 670 680 690 700 710 720 PVYCRPLVEK DPSTKLWCAA GVNLSGWKPH EEDSSNGPKP VPGRDPLTCD REGEGEPKST 730 740 750 760 770 780 HPSPEKKKAK ETPEADATSS RVWILTSTLT TSKVVIIDAN QPGTIVDQFT VCNAHVLCIS 790 800 810 820 830 840 SIPAASDSDY PPGEMFLDSD VNPEDSGADG VLAGITLVGC ATRCNVPRSN CSSRGDTPVL 850 860 870 880 890 900 DKGQGDVATT ANGKVNPSQS TEEATEATEV PDPGPSESEA TTVRPGPLTE HVFTDPAPTP 910 920 930 940 950 960 SSSTQPASEN GSESNGTIVQ PQVEPSGELS TTTSSAAPTM WLGAQNGWLY VHSAVANWKK 970 980 990 1000 1010 1020 CLHSIKLKDS VLSLVHVKGR VLVALADGTL AIFHRGEDGQ WDLSNYHLMD LGHPHHSIRC 1030 1040 1050 1060 1070 1080 MAVVNDRVWC GYKNKVHVIQ PKTMQIEKSF DAHPRRESQV RQLAWIGDGV WVSIRLDSTL 1090 1100 1110 1120 1130 1140 RLYHAHTHQH LQDVDIEPYV SKMLGTGKLG FSFVRITALL IAGNRLWVGT GNGVVISIPL 1150 1160 1170 1180 1190 1200 TETVVLHRGQ LLGLRANKTS PTSGEGTRPG GIIHVYGDDS SDKAASSFIP YCSMAQAQLC 1210 1220 1230 1240 1250 1260 FHGHRDAVKF FVSVPGNVLA TLNGSVLDSP SEGPGPAAPA ADAEGQKLKN ALVLSGGEGY 1270 1280 1290 1300 IDFRIGDGED DETEECAGDV NQTKPSLSKA ERSHIIVWQV SYTPE
	Isoform 1b [UniParc]. Checksum: 1EAD24AC1DAD9AB7	FASTA	1,314	145,251
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR SPRQSWRKSR KERPTSLNVF PLADGMCPND EMSESGQSSA 250 260 270 280 290 300 AATPSTTGTK SNTPTSSVPS AAVTPLNESL QPLGDYVSVT KNNKQAREKR NSRNMEVQVT 310 320 330 340 350 360 QEMRNVSIGM GSSDEWSDVQ DIIDSTPELD VCPETRLERT GSSPTQGIVN KAFGINTDSL 370 380 390 400 410 420 YHELSTAGSE VIGDVDEGAD LLGEFSVRDD FFGMGKEVGN LLLENSQLLE TKNALNVVKN 430 440 450 460 470 480 DLIAKVDQLS GEQEVLKGEL EAAKQAKVKL ENRIKELEEE LKRVKSEAVT ARREPREEVE 490 500 510 520 530 540 DVSSYLCTEL DKIPMAQRRR FTRVEMARVL MERNQYKERL MELQEAVRWT EMIRASREHP 550 560 570 580 590 600 SVQEKKKSTI WQFFSRLFSS SSSPPPAKRS YPSVNIHYKS PTAAGFSQRR SHALCQISAG 610 620 630 640 650 660 SRPLEFFPDD DCTSSARREQ KREQYRQVRE HVRNDDGRLQ ACGWSLPAKY KQLSPNGGQE 670 680 690 700 710 720 DTRMKNVPVP VYCRPLVEKD PSTKLWCAAG VNLSGWKPHE EDSSNGPKPV PGRDPLTCDR 730 740 750 760 770 780 EGEGEPKSTH PSPEKKKAKE TPEADATSSR VWILTSTLTT SKVVIIDANQ PGTIVDQFTV 790 800 810 820 830 840 CNAHVLCISS IPAASDSDYP PGEMFLDSDV NPEDSGADGV LAGITLVGCA TRCNVPRSNC 850 860 870 880 890 900 SSRGDTPVLD KGQGDVATTA NGKVNPSQST EEATEATEVP DPGPSESEAT TVRPGPLTEH 910 920 930 940 950 960 VFTDPAPTPS SSTQPASENG SESNGTIVQP QVEPSGELST TTSSAAPTMW LGAQNGWLYV 970 980 990 1000 1010 1020 HSAVANWKKC LHSIKLKDSV LSLVHVKGRV LVALADGTLA IFHRGEDGQW DLSNYHLMDL 1030 1040 1050 1060 1070 1080 GHPHHSIRCM AVVNDRVWCG YKNKVHVIQP KTMQIEKSFD AHPRRESQVR QLAWIGDGVW 1090 1100 1110 1120 1130 1140 VSIRLDSTLR LYHAHTHQHL QDVDIEPYVS KMLGTGKLGF SFVRITALLI AGNRLWVGTG 1150 1160 1170 1180 1190 1200 NGVVISIPLT ETVVLHRGQL LGLRANKTSP TSGEGTRPGG IIHVYGDDSS DKAASSFIPY 1210 1220 1230 1240 1250 1260 CSMAQAQLCF HGHRDAVKFF VSVPGNVLAT LNGSVLDSPS EGPGPAAPAA DAEGQKLKNA 1270 1280 1290 1300 1310 LVLSGGEGYI DFRIGDGEDD ETEECAGDVN QTKPSLSKAE RSHIIVWQVS YTPE
	Isoform 1d [UniParc]. Checksum: 362061FA2B2AA3C1	FASTA	1,336	147,474
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR RKERPTSLNV FPLADGMVRA QMGGKLVPAG DHWHLSDLGQ 250 260 270 280 290 300 LQSSSSYQCP NDEMSESGQS SAAATPSTTG TKSNTPTSSV PSAAVTPLNE SLQPLGDYVS 310 320 330 340 350 360 VTKNNKQARE KRNSRNMEVQ VTQEMRNVSI GMGSSDEWSD VQDIIDSTPE LDVCPETRLE 370 380 390 400 410 420 RTGSSPTQGI VNKAFGINTD SLYHELSTAG SEVIGDVDEG ADLLGEFSVR DDFFGMGKEV 430 440 450 460 470 480 GNLLLENSQL LETKNALNVV KNDLIAKVDQ LSGEQEVLKG ELEAAKQAKV KLENRIKELE 490 500 510 520 530 540 EELKRVKSEA VTARREPREE VEDVSSYLCT ELDKIPMAQR RRFTRVEMAR VLMERNQYKE 550 560 570 580 590 600 RLMELQEAVR WTEMIRASRE HPSVQEKKKS TIWQFFSRLF SSSSSPPPAK RSYPSVNIHY 610 620 630 640 650 660 KSPTAAGFSQ RRSHALCQIS AGSRPLEFFP DDDCTSSARR EQKREQYRQV REHVRNDDGR 670 680 690 700 710 720 LQACGWSLPA KYKQLSPNGG QEDTRMKNVP VPVYCRPLVE KDPSTKLWCA AGVNLSGWKP 730 740 750 760 770 780 HEEDSSNGPK PVPGRDPLTC DREGEGEPKS THPSPEKKKA KETPEADATS SRVWILTSTL 790 800 810 820 830 840 TTSKVVIIDA NQPGTIVDQF TVCNAHVLCI SSIPAASDSD YPPGEMFLDS DVNPEDSGAD 850 860 870 880 890 900 GVLAGITLVG CATRCNVPRS NCSSRGDTPV LDKGQGDVAT TANGKVNPSQ STEEATEATE 910 920 930 940 950 960 VPDPGPSESE ATTVRPGPLT EHVFTDPAPT PSSSTQPASE NGSESNGTIV QPQVEPSGEL 970 980 990 1000 1010 1020 STTTSSAAPT MWLGAQNGWL YVHSAVANWK KCLHSIKLKD SVLSLVHVKG RVLVALADGT 1030 1040 1050 1060 1070 1080 LAIFHRGEDG QWDLSNYHLM DLGHPHHSIR CMAVVNDRVW CGYKNKVHVI QPKTMQIEKS 1090 1100 1110 1120 1130 1140 FDAHPRRESQ VRQLAWIGDG VWVSIRLDST LRLYHAHTHQ HLQDVDIEPY VSKMLGTGKL 1150 1160 1170 1180 1190 1200 GFSFVRITAL LIAGNRLWVG TGNGVVISIP LTETVVLHRG QLLGLRANKT SPTSGEGTRP 1210 1220 1230 1240 1250 1260 GGIIHVYGDD SSDKAASSFI PYCSMAQAQL CFHGHRDAVK FFVSVPGNVL ATLNGSVLDS 1270 1280 1290 1300 1310 1320 PSEGPGPAAP AADAEGQKLK NALVLSGGEG YIDFRIGDGE DDETEECAGD VNQTKPSLSK 1330 AERSHIIVWQ VSYTPE
	Isoform 3a [UniParc]. Checksum: 8C71A02B7FF3FBB4	FASTA	1,322	145,785
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR SRKERPTSLN VFPLADGMVR AQMGGKLVPA GDHWHLSDLG 250 260 270 280 290 300 QLQSSSSYQC PNDEMSESGQ SSAAATPSTT GTKSNTPTSS VPSAAVTPLN ESLQPLGDYV 310 320 330 340 350 360 SVTKNNKQAR EKRNSRNMEV QVTQEMRNVS IGMGSSDEWS DVQDIIDSTP ELDVCPETRL 370 380 390 400 410 420 ERTGSSPTQG IVNKAFGINT DSLYHELSTA GSEVIGDVDE GADLLGEFSG MGKEVGNLLL 430 440 450 460 470 480 ENSQLLETKN ALNVVKNDLI AKVDQLSGEQ EVLKGELEAA KQAKVKLENR IKELEEELKR 490 500 510 520 530 540 VKSEAVTARR EPREEVEDDK IPMAQRRRFT RVEMARVLME RNQYKERLME LQEAVRWTEM 550 560 570 580 590 600 IRASREHPSV QEKKKSTIWQ FFSRLFSSSS SPPPAKRSYP SVNIHYKSPT AAGFSQRRSH 610 620 630 640 650 660 ALCQISAGSR PLEFFPDDDC TSSARREQKR EQYRQVREHV RNDDGRLQAC GWSLPAKYKQ 670 680 690 700 710 720 LSPNGGQEDT RMKNVPVPVY CRPLVEKDPS TKLWCAAGVN LSGWKPHEED SSNGPKPVPG 730 740 750 760 770 780 RDPLTCDREG EGEPKSTHPS PEKKKAKETP EADATSSRVW ILTSTLTTSK VVIIDANQPG 790 800 810 820 830 840 TIVDQFTVCN AHVLCISSIP AASDSDYPPG EMFLDSDVNP EDSGADGVLA GITLVGCATR 850 860 870 880 890 900 CNVPRSNCSS RGDTPVLDKG QGDVATTANG KVNPSQSTEE ATEATEVPDP GPSESEATTV 910 920 930 940 950 960 RPGPLTEHVF TDPAPTPSSS TQPASENGSE SNGTIVQPQV EPSGELSTTT SSAAPTMWLG 970 980 990 1000 1010 1020 AQNGWLYVHS AVANWKKCLH SIKLKDSVLS LVHVKGRVLV ALADGTLAIF HRGEDGQWDL 1030 1040 1050 1060 1070 1080 SNYHLMDLGH PHHSIRCMAV VNDRVWCGYK NKVHVIQPKT MQIEKSFDAH PRRESQVRQL 1090 1100 1110 1120 1130 1140 AWIGDGVWVS IRLDSTLRLY HAHTHQHLQD VDIEPYVSKM LGTGKLGFSF VRITALLIAG 1150 1160 1170 1180 1190 1200 NRLWVGTGNG VVISIPLTET VVLHRGQLLG LRANKTSPTS GEGTRPGGII HVYGDDSSDK 1210 1220 1230 1240 1250 1260 AASSFIPYCS MAQAQLCFHG HRDAVKFFVS VPGNVLATLN GSVLDSPSEG PGPAAPAADA 1270 1280 1290 1300 1310 1320 EGQKLKNALV LSGGEGYIDF RIGDGEDDET EECAGDVNQT KPSLSKAERS HIIVWQVSYT PE
	Isoform 1e [UniParc]. Checksum: AA7157299921450A	FASTA	1,345	148,587
	10 20 30 40 50 60 MMEIQMDEGG GVVVYQDDYC SGSVMSERVS GLAGSIYREF ERLIHCYDEE VVKELMPLVV 70 80 90 100 110 120 NVLENLDSVL SENQEHEVEL ELLREDNEQL LTQYEREKAL RKQAEEKFIE FEDALEQEKK 130 140 150 160 170 180 ELQIQVEHYE FQTRQLELKA KNYADQISRL EERESEMKKE YNALHQRHTE MIQTYVEHIE 190 200 210 220 230 240 RSKMQQVGGS GQTESSLPGR SPRQSWRKSR KERPTSLNVF PLADGMVRAQ MGGKLVPAGD 250 260 270 280 290 300 HWHLSDLGQL QSSSSYQCPN DEMSESGQSS AAATPSTTGT KSNTPTSSVP SAAVTPLNES 310 320 330 340 350 360 LQPLGDYVSV TKNNKQAREK RNSRNMEVQV TQEMRNVSIG MGSSDEWSDV QDIIDSTPEL 370 380 390 400 410 420 DVCPETRLER TGSSPTQGIV NKAFGINTDS LYHELSTAGS EVIGDVDEGA DLLGEFSVRD 430 440 450 460 470 480 DFFGMGKEVG NLLLENSQLL ETKNALNVVK NDLIAKVDQL SGEQEVLKGE LEAAKQAKVK 490 500 510 520 530 540 LENRIKELEE ELKRVKSEAV TARREPREEV EDVSSYLCTE LDKIPMAQRR RFTRVEMARV 550 560 570 580 590 600 LMERNQYKER LMELQEAVRW TEMIRASREH PSVQEKKKST IWQFFSRLFS SSSSPPPAKR 610 620 630 640 650 660 SYPSVNIHYK SPTAAGFSQR RSHALCQISA GSRPLEFFPD DDCTSSARRE QKREQYRQVR 670 680 690 700 710 720 EHVRNDDGRL QACGWSLPAK YKQLSPNGGQ EDTRMKNVPV PVYCRPLVEK DPSTKLWCAA 730 740 750 760 770 780 GVNLSGWKPH EEDSSNGPKP VPGRDPLTCD REGEGEPKST HPSPEKKKAK ETPEADATSS 790 800 810 820 830 840 RVWILTSTLT TSKVVIIDAN QPGTIVDQFT VCNAHVLCIS SIPAASDSDY PPGEMFLDSD 850 860 870 880 890 900 VNPEDSGADG VLAGITLVGC ATRCNVPRSN CSSRGDTPVL DKGQGDVATT ANGKVNPSQS 910 920 930 940 950 960 TEEATEATEV PDPGPSESEA TTVRPGPLTE HVFTDPAPTP SSSTQPASEN GSESNGTIVQ 970 980 990 1000 1010 1020 PQVEPSGELS TTTSSAAPTM WLGAQNGWLY VHSAVANWKK CLHSIKLKDS VLSLVHVKGR 1030 1040 1050 1060 1070 1080 VLVALADGTL AIFHRGEDGQ WDLSNYHLMD LGHPHHSIRC MAVVNDRVWC GYKNKVHVIQ 1090 1100 1110 1120 1130 1140 PKTMQIEKSF DAHPRRESQV RQLAWIGDGV WVSIRLDSTL RLYHAHTHQH LQDVDIEPYV 1150 1160 1170 1180 1190 1200 SKMLGTGKLG FSFVRITALL IAGNRLWVGT GNGVVISIPL TETVVLHRGQ LLGLRANKTS 1210 1220 1230 1240 1250 1260 PTSGEGTRPG GIIHVYGDDS SDKAASSFIP YCSMAQAQLC FHGHRDAVKF FVSVPGNVLA 1270 1280 1290 1300 1310 1320 TLNGSVLDSP SEGPGPAAPA ADAEGQKLKN ALVLSGGEGY IDFRIGDGED DETEECAGDV 1330 1340 NQTKPSLSKA ERSHIIVWQV SYTPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JSAP1, a novel jun N-terminal protein kinase (JNK)-binding protein that functions as a scaffold factor in the JNK signaling pathway." Ito M., Yoshioka K., Akechi M., Yamashita S., Takamatsu N., Sugiyama K., Hibi M., Nakabeppu Y., Shiba T., Yamamoto K. Mol. Cell. Biol. 19:7539-7548(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1A), FUNCTION, PHOSPHORYLATION, INTERACTION WITH MAPK8; MAPK9; MAPK10; MAP2K4 AND MAP3K1. Tissue: Brain.
[2]	"Isoforms of JSAP1 scaffold protein generated through alternative splicing." Ito M., Akechi M., Hirose R., Ichimura M., Takamatsu N., Xu P., Nakabeppu Y., Tadayoshi S., Yamamoto K., Yoshioka K. Gene 255:229-234(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1A; 1B; 1C; 1D AND 1E), TISSUE SPECIFICITY. Tissue: Brain.
[3]	"Interaction of a mitogen-activated protein kinase signaling module with the neuronal protein JIP3." Kelkar N., Gupta S., Dickens M., Davis R.J. Mol. Cell. Biol. 20:1030-1043(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORMS 1C AND 3A), FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION AT THR-266; THR-276 AND THR-287, MUTAGENESIS OF ARG-205; PRO-206; THR-207; SER-208; LEU-209; THR-266; THR-276 AND THR-287, INTERACTION WITH MAPK8IP2; MAPK8; MAPK9; MAPK10; MAP2K7 AND MAP3K11. Strain: C57BL/6. Tissue: Brain and Heart.
[4]	"Kinesin-dependent axonal transport is mediated by the Sunday Driver (SYD) protein." Bowman A.B., Kamal A., Ritchings B.W., Philp A.V., McGrail M., Gindhart J.G., Goldstein L.S.B. Cell 103:583-594(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1C), SUBCELLULAR LOCATION, INTERACTION WITH KLC1. Strain: C57BL/6. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1B), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1240-1337 (ISOFORM 1C). Strain: C57BL/6. Tissue: Brain.
[6]	"Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules." Verhey K.J., Meyer D., Deehan R., Blenis J., Schnapp B.J., Rapoport T.A., Margolis B. J. Cell Biol. 152:959-970(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KLC. Tissue: Brain.
[7]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, MASS SPECTROMETRY. Tissue: Brain cortex.
[9]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB005662 mRNA. Translation: BAA85874.1. AB043124 mRNA. Translation: BAB16675.1. AB043125 mRNA. Translation: BAB16676.1. AB043123 mRNA. Translation: BAB16674.1. AB043129 Genomic DNA. Translation: BAB16685.1. AF178637 mRNA. Translation: AAF26843.1. AF178636 mRNA. Translation: AAF26842.1. AF262046 mRNA. Translation: AAG36931.1. Different initiation. BC004003 mRNA. Translation: AAH04003.1. BC060603 mRNA. Translation: AAH60603.1.
IPI	IPI00230497. IPI00230498. IPI00230499. IPI00230500. IPI00230501. IPI00751423.
RefSeq	NP_001156919.1. NM_001163447.1. NP_001156920.1. NM_001163448.1. NP_001156921.1. NM_001163449.1. NP_001156923.1. NM_001163451.1. NP_038959.2. NM_013931.4.
UniGene	Mm.43081.
3D structure databases
ProteinModelPortal	Q9ESN9.
SMR	Q9ESN9. Positions 417-476, 775-815, 1091-1121.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9ESN9. 3 interactions.
PTM databases
PhosphoSite	Q9ESN9.
Proteomic databases
PaxDb	Q9ESN9.
PRIDE	Q9ESN9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000088345; ENSMUSP00000085683; ENSMUSG00000024163. ENSMUST00000115228; ENSMUSP00000110883; ENSMUSG00000024163. ENSMUST00000117509; ENSMUSP00000112712; ENSMUSG00000024163. ENSMUST00000119115; ENSMUSP00000112955; ENSMUSG00000024163. ENSMUST00000120035; ENSMUSP00000114084; ENSMUSG00000024163. ENSMUST00000146923; ENSMUSP00000114802; ENSMUSG00000024163.
GeneID	30957.
KEGG	mmu:30957.
UCSC	uc008aza.2. mouse. uc008azc.2. mouse. uc008aze.2. mouse. uc008azf.2. mouse.
Organism-specific databases
CTD	23162.
MGI	MGI:1353598. Mapk8ip3.
Phylogenomic databases
eggNOG	NOG270333.
GeneTree	ENSGT00670000097546.
HOVERGEN	HBG024110.
KO	K04436.
Gene expression databases
ArrayExpress	Q9ESN9.
Bgee	Q9ESN9.
Genevestigator	Q9ESN9.
GermOnline	ENSMUSG00000024163. Mus musculus.
Family and domain databases
Gene3D	2.130.10.10. 1 hit.
InterPro	IPR019143. JNK/Rab-associated_protein-1_N. IPR011047. Quinonprotein_ADH-like_supfam. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Pfam	PF09744. Jnk-SapK_ap_N. 1 hit. [Graphical view]
SUPFAM	SSF50998. Quin_alc_DH_like. 1 hit.
ProtoNet	Search...
Other
NextBio	307444.
SOURCE	Search...

Entry information

Entry name

JIP3_MOUSE

Accession

Primary (citable) accession number: Q9ESN9
Secondary accession number(s): Q5D062 , Q99KU7, Q9EQD8, Q9ESN7, Q9ESN8, Q9ESP0, Q9JLH2, Q9JLH3, Q9R0U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program