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Protein

Tripartite motif-containing protein 2

Gene

Trim2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance.2 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri23 – 64RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri113 – 154B box-typePROSITE-ProRule annotationAdd BLAST42

GO - Molecular functioni

  • myosin binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • cellular response to leukemia inhibitory factor Source: MGI
  • regulation of neuron apoptotic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite motif-containing protein 2 (EC:2.3.2.27)
Alternative name(s):
E3 ubiquitin-protein ligase TRIM2
Neural activity-related RING finger protein
RING-type E3 ubiquitin transferase TRIM2Curated
Gene namesi
Name:Trim2
Synonyms:Kiaa0517, Narf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1933163. Trim2.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutant mice are indistinguishable from wild type until about 1.5 months of age, when they begin to show intention tremor, followed by gait ataxia. At this stage, neurons exhibit axonal swellings, which consist of the accumulation of disorganized neurofilaments and microtubules, mitochondria and vesicles. In later stages, mutant animals suffer from episodes of spontaneous generalized seizures, a phenotype caused by progressive loss of Purkinje cells through apoptosis. At 4 months of age, retinas in mutant mice display decreased thickness of the inner nuclear layer and a reduced number of ganglionic cells. The outer plexiform layer is also reduced, whereas the size of the photoreceptor layer is not altered.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561961 – 744Tripartite motif-containing protein 2Add BLAST744

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Modified residuei371PhosphothreonineCombined sources1
Modified residuei375PhosphoserineCombined sources1
Modified residuei424PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1

Post-translational modificationi

RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9ESN6.
PaxDbiQ9ESN6.
PRIDEiQ9ESN6.

PTM databases

iPTMnetiQ9ESN6.
PhosphoSitePlusiQ9ESN6.

Expressioni

Tissue specificityi

Highly expressed in the cerebellum, hippocampus, retina and spinal cord. In the cerebellum, strongest expression in Purkinje cells and in the deep cerebellar nuclei. In retina, high expression in the ganglionic cell layer, inner nuclear layer and inthe outer plexiform layer. Particularly high expression in the hippocampus, in pyramidal cells of CA1-CA3 hippocampal areas and ingranule cells of the dentate gyrus.2 Publications

Developmental stagei

At 12.5 dpc, expressed in the developing nervous system, particularly in the spinal cord, dorsal rootganglia, hindbrain and midbrain.1 Publication

Gene expression databases

BgeeiENSMUSG00000027993.
CleanExiMM_NARF.
MM_TRIM2.
ExpressionAtlasiQ9ESN6. baseline and differential.
GenevisibleiQ9ESN6. MM.

Interactioni

Subunit structurei

Interacts with myosin V; myosin V may not be a substrate for ubiquitination. Interacts with NEFL. Interacts with phosphorylated BCL2L11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NeflP085512EBI-8315064,EBI-445199

GO - Molecular functioni

  • myosin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi219833. 5 interactors.
DIPiDIP-46254N.
IntActiQ9ESN6. 2 interactors.
MINTiMINT-4138547.
STRINGi10090.ENSMUSP00000069922.

Structurei

3D structure databases

ProteinModelPortaliQ9ESN6.
SMRiQ9ESN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati320 – 421FilaminAdd BLAST102
Repeati473 – 516NHL 1Add BLAST44
Repeati520 – 563NHL 2Add BLAST44
Repeati564 – 605NHL 3Add BLAST42
Repeati609 – 652NHL 4Add BLAST44
Repeati656 – 699NHL 5Add BLAST44
Repeati700 – 743NHL 6Add BLAST44

Domaini

The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri23 – 64RING-typePROSITE-ProRule annotationAdd BLAST42
Zinc fingeri113 – 154B box-typePROSITE-ProRule annotationAdd BLAST42

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410ITFR. Eukaryota.
ENOG410XQSU. LUCA.
GeneTreeiENSGT00890000139359.
HOGENOMiHOG000220817.
HOVERGENiHBG054843.
InParanoidiQ9ESN6.
KOiK11997.
PhylomeDBiQ9ESN6.
TreeFamiTF331018.

Family and domain databases

CDDicd00021. BBOX. 1 hit.
Gene3Di2.120.10.30. 2 hits.
2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR003649. Bbox_C.
IPR017868. Filamin/ABP280_repeat-like.
IPR001298. Filamin/ABP280_rpt.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR001258. NHL_repeat.
IPR013017. NHL_repeat_subgr.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF00630. Filamin. 1 hit.
PF01436. NHL. 6 hits.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
SMARTiView protein in SMART
SM00502. BBC. 1 hit.
SM00336. BBOX. 1 hit.
SM00557. IG_FLMN. 1 hit.
SM00184. RING. 1 hit.
SUPFAMiSSF81296. SSF81296. 1 hit.
PROSITEiView protein in PROSITE
PS50194. FILAMIN_REPEAT. 1 hit.
PS51125. NHL. 6 hits.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9ESN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEGASIPS PVVRQIDKQF LICSICLERY KNPKVLPCLH TFCERCLQNY
60 70 80 90 100
IPAHSLTLSC PVCRQTSILP EKGVAALQNN FFITNLMDVL QRTPGSNGED
110 120 130 140 150
SSILETVTAV AAGKPLSCPN HDGNVMEFYC QSCETAMCRE CTEGEHAEHP
160 170 180 190 200
TVPLKDVVEQ HKASLQVQLD AVNKRLPEID SALQFISEII HQLTNQKASI
210 220 230 240 250
VDDIHSTFDE LQKTLNVRKS VLLMELEVNY GLKHKVLQSQ LDTLLQGQES
260 270 280 290 300
IKSCSNFTAQ ALNHGTETEV LLVKKQMSEK LNELADQDFP LHPRENDQLD
310 320 330 340 350
FIVETEGLKK SIHNLGTILT TNAVASETVA TGEGLRQTII GQPMSVTITT
360 370 380 390 400
KDKDGELCKT GNAYLTAELS TPDGSVADGE ILDNKNGTYE FLYTVQKEGD
410 420 430 440 450
FTLSLRLYDQ HIRGSPFKLK VIRSADVSPT TEGVKRRVKS PGSGHVKQKA
460 470 480 490 500
VKRPASMYST GKRKENPIED DLIFRVGTKG RNKGEFTNLQ GVAASTSGKI
510 520 530 540 550
LIADSNNQCV QIFSNDGQFK SRFGIRGRSP GQLQRPTGVA VHPSGDIIIA
560 570 580 590 600
DYDNKWVSIF SNDGKFKTKI GSGKLMGPKG VSVDRNGHII VVDNKACCVF
610 620 630 640 650
IFQPNGKIVT RFGSRGNGDR QFAGPHFAAV NSNNEIIITD FHNHSVKVFN
660 670 680 690 700
QEGEFMLKFG SNGEGNGQFN APTGVAVDSN GNIIVADWGN SRIQVFDGSG
710 720 730 740
SFLSYINTSA DPLYGPQGLA LTSDGHVVVA DSGNHCFKVY RYLQ
Length:744
Mass (Da):81,445
Last modified:March 1, 2001 - v1
Checksum:i80336DA2EFEE5EFD
GO

Sequence cautioni

The sequence BAD90242 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043550 mRNA. Translation: BAB17634.1.
AF220017 mRNA. Translation: AAG53471.1.
AK042752 mRNA. Translation: BAC31352.1.
AK045410 mRNA. Translation: BAC32350.1.
AK079415 mRNA. Translation: BAC37640.1.
AK147275 mRNA. Translation: BAE27812.1.
AK220343 mRNA. Translation: BAD90242.1. Different initiation.
BC058961 mRNA. Translation: AAH58961.1.
CCDSiCCDS71256.1.
RefSeqiNP_001258654.1. NM_001271725.1.
NP_001258655.1. NM_001271726.1.
NP_001258656.1. NM_001271727.1.
NP_001258657.1. NM_001271728.1.
NP_109631.2. NM_030706.3.
XP_017175303.1. XM_017319814.1.
UniGeneiMm.44876.

Genome annotation databases

EnsembliENSMUST00000065380; ENSMUSP00000069922; ENSMUSG00000027993.
ENSMUST00000107691; ENSMUSP00000103319; ENSMUSG00000027993.
ENSMUST00000107692; ENSMUSP00000103320; ENSMUSG00000027993.
ENSMUST00000107693; ENSMUSP00000103321; ENSMUSG00000027993.
GeneIDi80890.
KEGGimmu:80890.
UCSCiuc008ppx.3. mouse.

Similar proteinsi

Entry informationi

Entry nameiTRIM2_MOUSE
AccessioniPrimary (citable) accession number: Q9ESN6
Secondary accession number(s): Q3UHP5, Q5DU27, Q8C981
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families