ID   CENPK_MOUSE             Reviewed;         271 AA.
AC   Q9ESN5; Q3UXA9; Q569Q3; Q8C469;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   24-JAN-2024, entry version 132.
DE   RecName: Full=Centromere protein K;
DE            Short=CENP-K;
DE   AltName: Full=SoxLZ/Sox6 leucine zipper-binding protein in testis;
GN   Name=Cenpk; Synonyms=Solt;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP   SPECIFICITY, AND POSSIBLE INTERACTION WITH SOX6.
RX   PubMed=10996314; DOI=10.1016/s0014-5793(00)01987-6;
RA   Yamashita A., Ito M., Takamatsu N., Shiba T.;
RT   "Characterization of Solt, a novel SoxLZ/Sox6 binding protein expressed in
RT   adult mouse testis.";
RL   FEBS Lett. 481:147-151(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC   STRAIN=C57BL/6J; TISSUE=Egg, and Spinal cord;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-120.
RC   STRAIN=NMRI; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the CENPA-CAD (nucleosome distal) complex, a
CC       complex recruited to centromeres which is involved in assembly of
CC       kinetochore proteins, mitotic progression and chromosome segregation.
CC       May be involved in incorporation of newly synthesized CENPA into
CC       centromeres via its interaction with the CENPA-NAC complex. Acts in
CC       coordination with KNL1 to recruit the NDC80 complex to the outer
CC       kinetochore (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the CENPA-CAD complex, composed of CENPI, CENPK,
CC       CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex
CC       interacts with the CENPA-NAC complex, at least composed of CENPA,
CC       CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. May interact with Sox6.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC       {ECO:0000250}. Chromosome, centromere, kinetochore {ECO:0000250}.
CC       Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is
CC       probably recruited on centromeres by the CENPA-NAC complex (By
CC       similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9ESN5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9ESN5-2; Sequence=VSP_020438;
CC       Name=3;
CC         IsoId=Q9ESN5-3; Sequence=VSP_020437, VSP_020439;
CC   -!- TISSUE SPECIFICITY: Highly expressed in testis.
CC       {ECO:0000269|PubMed:10996314}.
CC   -!- SIMILARITY: Belongs to the CENP-K/MCM22 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH92351.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
CC       Sequence=BAB16367.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB043687; BAB16367.1; ALT_INIT; mRNA.
DR   EMBL; AK082939; BAC38702.1; -; mRNA.
DR   EMBL; AK135773; BAE22654.1; -; mRNA.
DR   EMBL; BC092351; AAH92351.1; ALT_SEQ; mRNA.
DR   CCDS; CCDS26750.1; -. [Q9ESN5-2]
DR   RefSeq; NP_068562.1; NM_021790.2.
DR   RefSeq; NP_851406.1; NM_181061.5. [Q9ESN5-2]
DR   RefSeq; XP_017171041.1; XM_017315552.1.
DR   RefSeq; XP_017171042.1; XM_017315553.1.
DR   AlphaFoldDB; Q9ESN5; -.
DR   SMR; Q9ESN5; -.
DR   BioGRID; 208561; 20.
DR   ComplexPortal; CPX-5704; Kinetochore CCAN complex.
DR   IntAct; Q9ESN5; 1.
DR   MINT; Q9ESN5; -.
DR   STRING; 10090.ENSMUSP00000022227; -.
DR   iPTMnet; Q9ESN5; -.
DR   PhosphoSitePlus; Q9ESN5; -.
DR   MaxQB; Q9ESN5; -.
DR   PaxDb; 10090-ENSMUSP00000022227; -.
DR   PeptideAtlas; Q9ESN5; -.
DR   ProteomicsDB; 280060; -. [Q9ESN5-1]
DR   ProteomicsDB; 280061; -. [Q9ESN5-2]
DR   ProteomicsDB; 280062; -. [Q9ESN5-3]
DR   Antibodypedia; 23756; 216 antibodies from 26 providers.
DR   DNASU; 60411; -.
DR   Ensembl; ENSMUST00000070761.10; ENSMUSP00000070910.4; ENSMUSG00000021714.16. [Q9ESN5-2]
DR   GeneID; 60411; -.
DR   KEGG; mmu:60411; -.
DR   UCSC; uc007rtc.2; mouse. [Q9ESN5-2]
DR   UCSC; uc007rtd.2; mouse. [Q9ESN5-1]
DR   UCSC; uc007rtf.1; mouse. [Q9ESN5-3]
DR   AGR; MGI:1926210; -.
DR   CTD; 64105; -.
DR   MGI; MGI:1926210; Cenpk.
DR   VEuPathDB; HostDB:ENSMUSG00000021714; -.
DR   eggNOG; ENOG502QVGW; Eukaryota.
DR   GeneTree; ENSGT00390000006243; -.
DR   InParanoid; Q9ESN5; -.
DR   OrthoDB; 5399806at2759; -.
DR   PhylomeDB; Q9ESN5; -.
DR   TreeFam; TF333264; -.
DR   Reactome; R-MMU-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-MMU-2467813; Separation of Sister Chromatids.
DR   Reactome; R-MMU-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-MMU-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-MMU-606279; Deposition of new CENPA-containing nucleosomes at the centromere.
DR   Reactome; R-MMU-68877; Mitotic Prometaphase.
DR   Reactome; R-MMU-9648025; EML4 and NUDC in mitotic spindle formation.
DR   BioGRID-ORCS; 60411; 21 hits in 78 CRISPR screens.
DR   PRO; PR:Q9ESN5; -.
DR   Proteomes; UP000000589; Chromosome 13.
DR   RNAct; Q9ESN5; Protein.
DR   Bgee; ENSMUSG00000021714; Expressed in ureter smooth muscle and 178 other cell types or tissues.
DR   ExpressionAtlas; Q9ESN5; baseline and differential.
DR   GO; GO:0000939; C:inner kinetochore; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0007059; P:chromosome segregation; NAS:ComplexPortal.
DR   GO; GO:0051382; P:kinetochore assembly; IEA:InterPro.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   InterPro; IPR020993; Centromere_CenpK.
DR   PANTHER; PTHR14401; CENTROMERE PROTEIN K; 1.
DR   PANTHER; PTHR14401:SF6; CENTROMERE PROTEIN K; 1.
DR   Pfam; PF11802; CENP-K; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Centromere; Chromosomal rearrangement; Chromosome;
KW   Coiled coil; Kinetochore; Nucleus; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Centromere protein K"
FT                   /id="PRO_0000249483"
FT   COILED          11..44
FT                   /evidence="ECO:0000255"
FT   COILED          102..151
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..107
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_020437"
FT   VAR_SEQ         220..271
FT                   /note="MLINRMFDVPHDPYVKIRDSFWPPYIELLLRYGIALRHPEDPSQIRLEAFHQ
FT                   -> VPLRAMGSTLESCWFPRETLLEEAAFSLASASVG (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_020438"
FT   VAR_SEQ         266..271
FT                   /note="LEAFHQ -> TSCMVGACGDQKSHH (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:16141072"
FT                   /id="VSP_020439"
FT   CONFLICT        115
FT                   /note="S -> T (in Ref. 3; AAH92351)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   271 AA;  31686 MW;  0805A7930078B014 CRC64;
     MSENKQEVHP DTITDVEAVI DTEEELIKEC EEMWKDMEDC QNKLSLIGTE TLTNADAQLS
     LLIMQMKCLT AELGQWKKRK PEIIPLNEDV LLTLGKEEFQ KLRCDLEMVL STIQSKNEKL
     KEDLEREQQW LDEQQQILDT LNVLNSDVEN QVVTLTESRI FNELTTKIRG IKEFKEKLLL
     TLGAFLDNHF PLPEASTPKK RKNIQDSNAQ LITLNEILEM LINRMFDVPH DPYVKIRDSF
     WPPYIELLLR YGIALRHPED PSQIRLEAFH Q
//