Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hyaluronan and proteoglycan link protein 2

Gene

Hapln2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates a firm binding of versican V2 to hyaluronic acid. May play a pivotal role in the formation of the hyaluronan-associated matrix in the central nervous system (CNS) which facilitates neuronal conduction and general structural stabilization. Binds to hyaluronic acid.

GO - Molecular functioni

GO - Biological processi

  • cell adhesion Source: GO_Central
  • central nervous system development Source: GO_Central
  • establishment of blood-nerve barrier Source: MGI
  • extracellular matrix assembly Source: MGI
  • skeletal system development Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Hyaluronic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan and proteoglycan link protein 2
Alternative name(s):
Brain link protein 1
Gene namesi
Name:Hapln2
Synonyms:Bral1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2137300. Hapln2.

Subcellular locationi

GO - Cellular componenti

  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 341314Hyaluronan and proteoglycan link protein 2PRO_0000013188Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi58 ↔ 129By similarity
Disulfide bondi171 ↔ 241By similarity
Disulfide bondi195 ↔ 216By similarity
Disulfide bondi266 ↔ 337By similarity
Disulfide bondi291 ↔ 312By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9ESM3.
PaxDbiQ9ESM3.
PRIDEiQ9ESM3.

Expressioni

Tissue specificityi

Brain. Predominantly expressed by neurons. Colocalizes with versican V2 in developing and adult cerebellar white matter and at the nodes of Ranvier.1 Publication

Developmental stagei

Expression starts at postnatal day 20 and increases thereafter.

Gene expression databases

BgeeiQ9ESM3.
CleanExiMM_HAPLN2.
ExpressionAtlasiQ9ESM3. baseline and differential.
GenevisibleiQ9ESM3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005014.

Structurei

3D structure databases

ProteinModelPortaliQ9ESM3.
SMRiQ9ESM3. Positions 45-134, 150-242, 256-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 143109Ig-like V-typeAdd
BLAST
Domaini149 – 24395Link 1PROSITE-ProRule annotationAdd
BLAST
Domaini246 – 33994Link 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HAPLN family.Curated
Contains 2 Link domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IIS5. Eukaryota.
ENOG410XVBZ. LUCA.
GeneTreeiENSGT00760000119025.
HOGENOMiHOG000234353.
HOVERGENiHBG051922.
InParanoidiQ9ESM3.
KOiK06851.
OMAiCFTSALA.
OrthoDBiEOG7ZWD1D.
PhylomeDBiQ9ESM3.
TreeFamiTF332134.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 2 hits.
InterProiIPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR000538. Link_dom.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF56436. SSF56436. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ESM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSRIPLPAF CCFLLPWAFT SFHKALGNPA PHPGPHYLLP PIHEVIHSRR
60 70 80 90 100
GATATLPCVL GTSPPSYKVR WSKVEPGELR ETLILITNGL HARDYGLLGG
110 120 130 140 150
RASLRRGHRL DASLIIKNVR LEDEGRYRCE LINGIEDESV ALTLRLEGVV
160 170 180 190 200
FPYQPSRGRY QFNYFEAKRA CEEQDGRLAT YGQLYQAWTE GLDWCNAGWL
210 220 230 240 250
LEGSVRYPVL TARAPCGGHG RPGIRSYGPR DRSRDRYDAF CFTSALAGQV
260 270 280 290 300
FFVPGRLTLS EAHAACRRRG AVVAKVGHLY AAWKFSGLDQ CDGGWLADGS
310 320 330 340
VRFPITTPRP RCGGLPDPGV RSFGFPRPQQ ASYGTYCYAE K
Length:341
Mass (Da):37,925
Last modified:March 1, 2001 - v1
Checksum:i1F65DCD07B5CFF02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049055 mRNA. Translation: BAB17663.1.
CCDSiCCDS17463.1.
RefSeqiNP_071314.1. NM_022031.2.
XP_006502211.1. XM_006502148.2.
UniGeneiMm.294467.

Genome annotation databases

EnsembliENSMUST00000005014; ENSMUSP00000005014; ENSMUSG00000004894.
GeneIDi73940.
KEGGimmu:73940.
UCSCiuc008ptq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB049055 mRNA. Translation: BAB17663.1.
CCDSiCCDS17463.1.
RefSeqiNP_071314.1. NM_022031.2.
XP_006502211.1. XM_006502148.2.
UniGeneiMm.294467.

3D structure databases

ProteinModelPortaliQ9ESM3.
SMRiQ9ESM3. Positions 45-134, 150-242, 256-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005014.

Proteomic databases

MaxQBiQ9ESM3.
PaxDbiQ9ESM3.
PRIDEiQ9ESM3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005014; ENSMUSP00000005014; ENSMUSG00000004894.
GeneIDi73940.
KEGGimmu:73940.
UCSCiuc008ptq.2. mouse.

Organism-specific databases

CTDi60484.
MGIiMGI:2137300. Hapln2.

Phylogenomic databases

eggNOGiENOG410IIS5. Eukaryota.
ENOG410XVBZ. LUCA.
GeneTreeiENSGT00760000119025.
HOGENOMiHOG000234353.
HOVERGENiHBG051922.
InParanoidiQ9ESM3.
KOiK06851.
OMAiCFTSALA.
OrthoDBiEOG7ZWD1D.
PhylomeDBiQ9ESM3.
TreeFamiTF332134.

Miscellaneous databases

ChiTaRSiHapln2. mouse.
PROiQ9ESM3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9ESM3.
CleanExiMM_HAPLN2.
ExpressionAtlasiQ9ESM3. baseline and differential.
GenevisibleiQ9ESM3. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.100.10. 2 hits.
InterProiIPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
IPR000538. Link_dom.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
PF00193. Xlink. 2 hits.
[Graphical view]
PRINTSiPR01265. LINKMODULE.
SMARTiSM00409. IG. 1 hit.
SM00408. IGc2. 1 hit.
SM00406. IGv. 1 hit.
SM00445. LINK. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF56436. SSF56436. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS01241. LINK_1. 2 hits.
PS50963. LINK_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The brain link protein-1 (BRAL1): cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain."
    Hirakawa S., Oohashi T., Su W.-D., Yoshioka H., Murakami T., Arata J., Ninomiya Y.
    Biochem. Biophys. Res. Commun. 276:982-989(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  2. "Bral1, a brain-specific link protein, colocalizing with the versican V2 isoform at the nodes of Ranvier in developing and adult mouse central nervous systems."
    Oohashi T., Hirakawa S., Bekku Y., Rauch U., Zimmermann D.R., Su W.-D., Ohtsuka A., Murakami T., Ninomiya Y.
    Mol. Cell. Neurosci. 19:43-57(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHPLN2_MOUSE
AccessioniPrimary (citable) accession number: Q9ESM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.